HONE1 Cell：人鼻咽癌复苏传代细胞系 货号：GD-C6519633
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Sudan Ⅰ
Reserpine
N-EthylheptylaMine , 98%
2H-PERFLUORO-5,8,11,14-TETRAMETHYL-3,6,9,12,15-PENTAOXAOCTADECANE
1-CHLORO-3-METHYLBUTANE
3,3-DiMethoxypropanenitrile
2,5-Dinitrophenol (wetted with ca. 20% Water, containing 5g on a dry weight basis)
1-Methyl-5-phenyl-1H-pyrazole-3-carboxylic acid , 97%
(S)-3,5 DHPG
trans-tert-Butyl 6-amino-3-azabicyclo[3.1.0]-hexane-3-carboxylate
3-Hexylthiophene
Methamidophos
Alpinetin
Triphosphopyridine nucleotide disodium salt
1-Ethynyl-4-propylbenzene
4-Hydroxy-6-MethoxyMethyl-2-(Methylthio)pyriMidine
1-EthyladaMantane
3-(4-Nitrophenoxy)benzaldehyde
5-BroMobenzo[b]thiophene-3-carboxylic acid, 97%
BROMOFORM
2,2-DiMethylhexanoic Acid
4-Acetylpyridine
Lithium amide
2,3,5,6-Tetrafluoroterephthalonitrile
4-phosphonobenzoic acid
1-Methylpyrrolidine
Norfloxacin
4-Hydroxy-2,6-Tetra-Methyl-1-Piperidine-ethanol,BW-10LD (622)
ZirconiuM(IV) 1,1,1-trifluoro-2,4-pentanedionate, 98+%
Methyl 3-(2-broMoethoxy)benzoate, 97+%
5-BroMo-6-chloronicotinic acid
HeMatoxylin
3-BroMobenziMidaMide, HCl
N,N-DiMethylglycine Methyl ester, 98%
BenzyltriMethylaMMoniuM hydroxide
BenzyltriMethylaMMoniuM chloride
1,2-Bis(trifluoromethyl)benzene
5-Bromo-2-methoxyphenylacetonitrile
2-Bromo-1-(5-chloro-3-methylbenzo[b]thiophen-2-yl)ethan-1-one , 95%
5-(4-Methylphenyl)-1,2,4-thiadiazole
Perindopril
2-NITRONAPHTHALENE
Isoprothiolane
N-t-BOC-D-alanine;N-tert-Butoxycarbonyl-D-alanine;N-tert-Butoxycarbonyl-(R)-2-aMinopropionic acid;BOC-D-Ala
2-Chloroethyl-p-toluenesulfonate
Methyl 5-(AMinosulfonyl)-2-Methoxybenzoate
Pioglitazone Hydrochloride
1,2-DibroMobenzene, 98%
Kojic acid, 99%
(R)-2-Amino-2-methyl-4-pentenoic acid
(+/-)-Hydrobenzoin
3,6-dibromo-9-phenyl-9H-carbazole
tert-butyl (2-hydroxy-1-phenylethyl)carbamate
6-Bromonicotinic acid methyl ester
N-succinimidyl palmitate
(4-METHYLPHENOXY)ACETIC ACID
PTH-L-PROLINE
Fluorescein isothiocyanate isoMer I
ForMaldehyde
Ethyl 3-nitrobenzoate , tech
2-hyrazinyl-6-bromobenzothiazole
4-[(butylthio)methyl]-5-methyl-2-furoic acid(SALTDATA: FREE)
Chloro(2-dicyclohexylphosphino-2',6'-dimethoxy-1,1'-biphenyl)[2-(2-aminoethylphenyl)]palladium(II) methyl-t-butylether adduct, min. 98% [SPhos Palladacycle]
Hydrazinium hydroxide
4,6-Dichloronicotinic acid
N-(2-CHLOROETHYL)ACETAMIDE
Paclitaxel Related CoMpound B;10-Deacetyl-7-epipaclitaxel
2-Chloropyrazine
2,2'-Benzidinedisulfonic Acid (contains 30% Water at MaxiMuM)
3-BroMo-5-butoxyphenylboronic acid
5-BroMosalicylic acid, 98%
2-Naphthonitrile, 97%
Tyramine hydrochloride
DiMethyl Octadecanedioate
3-Hydroxybenzothiophene
3-AMINO-2-METHOXY-6-PICOLINE
5-Cyanophthalide
4-Cyano-4-(2-Methoxyphenyl)Cyclohexanone
FIX ELISA Kit
BF ELISA Kit
Human Glucagon，GC ELISA Kit
Human VDAC1 ELISA Kit
TTR ELISA Kit
Rat Interleukin 5,IL-5 ELISA Kit
IL8 ELISA Kit
HDV IgG ELISA Kit
CO ELISA Kit
Mouse 8-iso prostaglandin,8-iso-PG ELISA Kit
Broken Meat Medium Base
Agrobacterium rhizogene Broth Medium
Ethyl 2-BroMopropionate
trans-4-Hydroxy-L-proline Methyl ester hydrochloride, 98%
4-AcetaMidothiophenol, 98% (dry wt.), water <5%
2-AMinoMethylpyriMidine Hydrochloride
4-Acetylpyridine
SODIUM OLEATE
Potassium tetracyanonickelate(II) hydrate
trans-1,4-cyclohexanedicarboxylic acid
SILVER PERCHLORATE
INDIUM ACETYLACETONATE
(5R,6S)-(-)-4-Benzyloxycarbonyl-5,6-diphenyl-2-Morpholinone,98%
(-)-1,2-Bis[(2R,5R)-2,5-diethylphospholano]benzene(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate, 97%
2-BroMo-4-nitropyridine, 94%
DysprosiuM
DiethylaMinosulfur trifluoride
Thiourea dioxide
5-AMino-6-nitroquinoline
CesiuM hydroxide Monohydrate
4-(TERT-BUTYLDIMETHYLSILYLOXY)PHENYLBORONIC ACID
3,9-Bis[2-(3,5-diamino-2,4,6-triazaphenyl)ethyl]-2,4,8,10-tetraoxaspiro[5.5]undecane
1,3-Difluoroacetone 98%
3-Acetoxybenzofuran, 99%
4-(1-Hydroxypropyl)benzotrifluoride 97%
