SW 1088[SW-1088,SW1088] Cell：人脑星型胶质瘤复苏贴壁细胞系

SW 1088[SW-1088,SW1088] Cell：人脑星型胶质瘤复苏贴壁细胞系 货号：GD-C6420567
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-(TrifluoroMethoxy)benzylaMine, 97%
1-Chloro-3-nitrobenzene solution
2,6-Difluorobenzyl alcohol
8-Mercaptoquinoline hydrochloride
2,6-Di-tert-butyl-4-diMethylaMinoMethylphenol
o-Acetoacetaniside
ALLYLDIPHENYLPHOSPHINE
L-Cystine dihydrochloride
Ethyl fluoroacetate
3-Bromo-4-hydroxybenzyl alcohol
N-2-Nitrophenylsulfenyl-L-isoleucine Dicyclohexylammonium Salt
3,5-Dibromo-2-pyridylamine
P-TOLUIC HYDRAZIDE
3,5-Diisopropylbenzene hydroperoxide
2-Chloro-3-pyridinamine
4,4'-Bis(diethylaMino)benzophenone
Diethyl Dodecanedioate
4-(4-BroMo-1H-pyrazol-1-yl)-6-chloropyriMidine
Stearyl stearate, 98%
MegluMine, USP
2-Phenoxybenzyl Chloride
(-)-alpha-cedrene
4-methylphthalic acid
5-methylhexanoic acid
tert-Butyl disulfide
2-Butylfuran
2,5-Difluorobenzoic acid
Ethylene glycol, 99%
2-Methyl-5-(trifluoroMethyl)benzonitrile, 97%
2-(MethylsulfonylaMino)benzeneboronic acid, 95%
3-Chloro-5-fluorobenzonitrile
D-alpha-Cyclohexylglycine
Cyclohexanone Tosylhydrazone
tert-Butyl Glycidyl Ether , 96%
4-BroMo-(2-Methoxyethoxy)anisole
4-(3-Piperidinyloxy)benzaMide, 96%
Fenobam
(S)-(-)-1,1,1-Trifluoroheptan-2-ol (>98%ee)
N-Formylglycine Ethyl Ester
Tetrabenzoporphine
METHYL 2-METHYL-3-FUROATE
Barium permanganate
1-[3-(TRIFLUOROMETHYL)PYRID-2-YL]
Trichloroacetyl chloride
2-Chlorophenol
N-Benzyl-N,N'-diMethylethylenediaMine
3,5-Difluoro-2-nitrophenylboronic acid
Cyclohexane-d12, for NMR, 99.5 atoM % D
(S)-N-BOC-(3-Pyridyl)alanine, 95%, 98% ee
3-(2-AMinoethyl)pyridine
N-(TriMethylsilyl)bis(trifluoroMethanesulfonyl)iMide
5-chloro-2-methylaminobenzophenone
Boronic acid,B-(5-bromo-2-chloro-4-pyridinyl)-
2-PHENYLIMIDAZO[1,2-A]PYRIDINE-3-CARBOXYLIC ACID
sulconazole nitrate
(2R,3R)-(+)-BIS(DIPHENYLPHOSPHINO)BUTANE
4-(3,3-DIMETHYL-1,5-DIOXASPIRO[5,5]UNDEC-9-YL) CYCLOHEXANONE
Pyrido[2,3-b]pyrazin-7-ylboronic acid, pinacol ester
2-ChloroMethyl-4-Methoxy-3,5-diMethylpyridine hydrochloride
Allyltris(triMethylsilyloxy)silane , 96%
2-(Di-tert-butylphosphino)-2'-methylbiphenyl ,99%
(S)-2,2'-Dimethoxy-1,1'-binaphthyl
1-Octadecyne
Dimethylammonium Dimethyldithiocarbamate
Novaluron
3-Bromobenzyl bromide
Disodium 1,5-naphthalenedisulfonate
2-Dicyclohexylphosphino-2'-(N,N-dimethylamino)biphenyl
1-(2-Pyrazinyl)
N-(3-DiMethylaMinopropyl)MethacrylaMide (stabilized with MEHQ)
6-BroMo-2-Methyl-3-nitropyridine
Propyl p-toluenesulfonate, 99%
HEPTANE
Tetrahydroxy-1,4-benzoquinone DisodiuM Salt
6-Azaindole
5-formylsalicylaldehyde
3-Methyl-1-butene
phthalamic acid
Trypsin ELISA Kit
IL23 ELISA Kit
Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA Kit
PHC ELISA Kit
DRD4 ELISA Kit
CD30 ELISA Kit
IFN-β ELISA Kit
Human HNRPU ELISA Kit
ACA ELISA Kit
NTRK2 ELISA Kit
MacConkey Agar
Casein Agar
Zinc protoporphyrin, 96%
AMMoniuM peroxydisulfate, 98%
5-Hexenoic acid, 99%
2-Methylthiophene, 98%
(-)-alpha-cedrene
1,2-dimethoxypropane
2-(2-ethoxyphenoxy)ethylamine
pyridaphenthion

2-CYCLOPENTENE-1-ACETIC ACID
CYCLOHEPTYLAMINE
6-AMinoindazole
Bis(4-Methoxyphenyl)phenylphosphine, 95%
Arachidic acid, 98%
ChroMotrope 2B
Tetraethylene glycol diMethyl ether
2,6-DIMETHYLPHENYL ISOCYANIDE
IRON(II) PERCHLORATE HYDRATE, 98%
N-(4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,11-Heptadecafluoroundecyl)iodoacetaMide
1-BroMo-3-cyclobutylthiobenzene
2-Chlorophenylacetyl chloride, 97%
Cyclopropylmagnesium Bromide (ca. 10% in Tetrahydrofuran, ca. 0.7mol/L)
Cholesterol, stabilized, 95%
DL-Ethyl 2-bromovalerate, 99%