HTSMC Cell：人气管平滑肌复苏贴壁细胞系

HTSMC Cell：人气管平滑肌复苏贴壁细胞系 货号：GD-C6419647
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Fluoro-4-Methylphenylboronic acid
3-Fluoro-4-Methoxybenzaldehyde
Gold wire, arsenic doped, 0.2MM (0.008in) dia, 99.99% (Metals basis)
N,N'-BIS-Z-1-GUANYLPYRAZOLE
3-BROMO-5-(TRIFLUOROMETHYL)BENZENESULFONYL CHLORIDE
4-tert-Butylbenzonitrile
4-Methylthiophenylisothiocyanate
4'-Fluorochalcone
3-Bromobenzyl bromide
N-Benzyl-N-methylethanolamine, 90%
CALCIUM TARTRATE
4-Nitrophthalic acid
ALLOXAN MONOHYDRATE
COBALT (II) PERCHLORATE
2,5-Di(2,2,2-trifluoroethoxy)benzene-1-carbohydrazide
SodiuM hypochlorite
(3S)-(-)-3-(EthylaMino)pyrrolidine
2-(4-Pyridinyl)benzaldehyde
6-Hydroxynicotinic acid, 99%
Carbon AtoMic Absorption Standard
2-Chloro-3-Methylpyridine
4-(Methylthio)thiophenol
mercurous sulfate
3-(TRIFLUOROMETHYL)PHENYL ISOTHIOCYANATE
5-Hydroxyisophthalic acid
2,6-Dichlorophenylhydrazine hydrochloride
N,N-DIMETHYLHEXYLAMINE
Octyl Acetate
ZirconiuM dichloride oxide octahydrate, 98%
2-Iodo-4-(trifluoroMethyl)phenol, 97%
9-BroMononanoic Acid
2-BroMo-5-fluorobenzyl broMide
1-Hexyl-3-MethyliMidazoliuM BroMide , 98%
N,N-DiMethyl 3-broMobenzenesulfonaMide
ETHYL PERFLUOROOCTANOATE
Z-Phe-OBzl
1-Bromobutane
4-N-Phthaloylglyaminomethyl aniline
1-[2-(Methylthio)phenyl]-2-thiourea
N-Benzyl-D-Proline ethyl Ester
(Carbomethoxymethyl)triphenylphosphonium bromide
2,1,3-Benzothiadiazole-5-carboxylic acid
1,6-HEXANEDIAMINE
4-Cyanophenylboronic acid
1-Methyl-4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)-1H-indole
5-Chloro-1-benzothiophene-3-carbonitrile
4-(3-Hydroxyphenoxy)benzoic Acid
2-Fluoro-4-Methoxypyridine
2,3,4-Trifluorobenzene-1-sulfonyl chloride, 97%
Guanidine thiocyanate
3-BroMocatechol
pyrene-1-carboxaldehyde
1H-Benzimidazole, 1-[(4-methylphenyl)sulfonyl]-
bromopride
nystatin dihydrate
1-Chloro-3-fluorobenzene
N-Propyl 4-broMobenzaMide
2-(2-Methoxyphenoxy)-N-MethylethylaMine, 96%
FMoc-β-(2-pyridyl)-D-Ala-OH
Anthracene, 99%
1-(3,5-Dimethyl-1H-pyrrol-2-yl)ethan-1-one, 97%
6-Methoxy-1H-indole-2-boronic acid, N-BOC protected 98%
Phenolphthalein disulfate, tripotassium salt trihydrate, pure, 98%
4,4'-AZOXYDIPHENETOLE
5-CHLORO-2-(TRIFLUOROMETHYL)BENZYL BROMIDE
AMINOMALONONITRILE P-TOLUENESULFONATE
2,4-Diaminopyrimidine
Cupferron
2,2-Bis(3,4-diMethylphenyl)hexafluoropropane
Ethyl 1-(3-chloropyridin-2-yl)-5-Methylpyrazole-4-carboxylate
alpha,alpha'-Dichloro-p-xylene, 98%
trans-4-BroMo-beta-nitrostyrene, 99%
1-BroMo-5-chloropentane
1,2,3,4-Cyclopentanetetracarboxylic Acid
4-Methoxybenzophenone
2-(3-ethylphenyl)ethyl-trimethoxy-silane
4-Fluoro-2-(trifluoromethyl)benzaldehyde
tetrakis(2,2,6,6-tetramethyl-4-piperidyl) butane-1,2,3,4-tetracarboxylate
FⅡ ELISA Kit
Mouse BAFFR (B-Cell Activation Factor Receptor) ELISA Kit
Human Amylin ELISA Kit
Resistin ELISA Kit
MHC I/RLA I ELISA Kit
IL7 ELISA Kit
IFNγ ELISA Kit
Human toxic shock syndrome toxin,TSST-1 ELISA Kit
Human TRY (Trypsin) ELISA Kit
CTX-Ⅰ ELISA Kit
Eubacterium Selective Agar
Azospirillum Broth Medium
Ethyl 4-chloro-5,8-diMethoxyquinoline-3-carboxylate, 97%
trans-4-n-Butyl-4'-(4-n-pentylcyclohexyl)biphenyl, 98%
4-AcetaMidophenol, 98%
2-AMino-Oxazole-5-Carboxylic Acid Ethyl Ester
4-(Methylthio)thiophenol
Synephrine Tartrate
Rubber accelerator CED
m-Tolunitrile

2'-CHLORO-4'-FLUOROACETOPHENONE
Allyl phenoxyacetate
Phenylhydrazine Sulfate
1-Cyclohexene-1-boronic acid pinacol ester, 95%
18-Pentatriacontanone, 97+%
4,5-Dichloro-3-hydroxypyridazine
4-Pyridinecarboxaldehyde
TriacetylMethane
Tolylene-2,4-diisothiocyanate
1,4,7,10-Tetrakis(carboxyMethyl)-1,4,7,10-tetraazacyclotridecane
2-n-Octylthiophene, 98%
1-Ethyl-2-iodo-3-methylbenzene
3-Acetylthianaphthene ,98%
2-Fluoro-3-(trifluoromethyl)phenylacetic acid
2-(4,5-Dimethoxy-2-nitrophenyl)acetic acid, 95%