HCT116 Cell：人结直肠癌复苏贴壁细胞系

HCT116 Cell：人结直肠癌复苏贴壁细胞系 货号：GD-C6419611
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Leaf Alcohol
2-Chloro-4-(trifluoroMethyl)benzonitrile
Diisopropyl(3,3,4,4,5,5,6,6,6-nonafluorohexyl)silane
BerbaMine dihydrochloride
2,2'-BITHIOPHENE
4-(2-Morpholinoethoxy)phenylboronic acid, pinacol ester
3,4-Dihydro-2H-1,4-benzoxazin-2-ylmethanol, 97%
Methyl 2-Acetamidoacrylate
1-(4-methyl-1H-imidazol-2-yl)methanamine(SALTDATA: 1.95HCl 0.2H2O)
3,6-Dihydro-2H-pyridine-1-N-Boc-4-boronic acid neopentylglycol ester
Ethyl 3-(chloroformyl)propionate
Deoxycorticosterone acetate
TRIPHENYLARSINE
Alibendol
SodiuM hydrosulfide hydrate
Methyl 3-aMino-4-Methylbenzoate
N-Ethoxycarbonyl-3-nitro-o-toluidine
Methyl 4-(5-forMylfuran-2-yl)benzoate
Thallic acetate, 98%
BENZO(B)FLUORANTHENE
Isobutyl Methacrylate (stabilized with HQ)
2-Hydroxythiophenol
Zinc nitrate
Trimethyl-n-propoxysilane
2,6-Dinitrotoluene
3,4-DINITROANILINE
CHLOROPLATINIC ACID HEXAHYDRATE
BariuM chloride, ultra dry, 99.95% (Metals basis)
Pinacolone oxiMe, 98%
2-Fluoro-5-nitrobenzoic acid, 98%
trans-1,4-Cyclohexanedicarboxylic acid
POTASSIUM PHENETHYLTRIFLUOROBORATE 95
2-Chloro-6-fluoroquinoline
N-[α-(4-Methylphenyl)sulfonyl)benzyl]diphenylphosphinic aMide
D-(-)-ALPHA-PHENYLGLYCINE ETHYL ESTER HYDROCHLORIDE
1-BENZYL-4-(ETHOXYCARBONYLMETHYL)
3-(Cyclopentyloxy)-4-methoxybenzoic acid
2-Chloro-5-fluorobenzyl alcohol
3-Iodo-o-xylene
tert-Butyl (2-aminophenyl)carbamate
3,4-Epoxytetrahydrofuran
2,4-Dichloro-5-nitrophenol
Tetrachloropropene
3-Fluorophenol
2',5'-Dichloroacetophenone
1-Benzyl N-(tert-Butoxycarbonyl)-L-glutaMate
4'-Ethylbiphenyl-3-carboxylic acid
Benzoyl broMide, 97%
N-(tert-Butoxycarbonyl)-L-alanine N'-Methoxy-N'-MethylaMide, 98%
1-(M-Tolyl)
2',4'-Difluoroacetophenone
2,3,5,6-Tetrafluorobenzoic acid
N-Boc-3-piperidinepropionic acid
sodium phenothiazine-10-yl-propylsulfonate
1-bromo-7-chloroheptane
1,1,2,2-Tetrafluoroethyl methyl ether
1-NITRO-4-N-PROPYLBENZENE
3-(Cbz-AMino)-5-fluorophenylboronic acid
4-METHOXY-2-(TRIFLUOROMETHYL)BENZOIC ACID
Benzyl 2-chloro-4-(trifluoromethyl)pyrimidine-5-carboxylate 97%
Trifluoroacetyl triflate, 90%
Triethylsulfonium Bis(trifluoromethanesulfonyl)imide
5-Methoxyindole-3-acetonitrile
HEXACHLORODISILOXANE
GENITE
6-PIPERAZINONICOTINONITRILE
Demeclocycline hydrochloride
tert-Butyl Methyl ether
2,2,2-Trichloroethanol
1H-Tridecafluorohexane
2-Mercaptonicotinic acid, 98%
3,5-DiMethyl-4-isoxazolecarboxaMide, 97%
Benzophenone
(1-Chloroethyl)benzene
1,2,3,4,5-cyclopentanepentayl, compd. with 1-[(1S)-1-(diphen...
3,5-dichloro-4-(1,1,2,2-tetrafluoroethoxy)aniline
Ethyl 2-amino-4-thiazoleacetate
4-Chloro-4-hydroxybiphenyl
Cortisol ELISA Kit
Mouse Cluster of differentiation 19,CD19 ELISA Kit
Human CA242 (Pancreatic Carcinoma Markers-CA242) ELISA Kit
Human FE65 ELISA Kit
TPS ELISA Kit
IL-32 ELISA Kit
SELE ELISA Kit
Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) ELISA Kit
Human Folic acid,FA ELISA Kit
Mouse 6-keto-prostaglandin,6-K-PG ELISA Kit
XLT4 Agar
MacConey Agar
Diethyl ethylMalonate, 99%
Crotonyl Chloride, ReMainder Mainly Cis-IsoMer
Methyl 4-acetylbenzoate, 99%
2-Aminothiazole
2-Hydroxythiophenol
2-Methoxynicotinic acid
4,5-Diaminopyrimidine
3-Nitrobenzyl chloride

11-HYDROXYUNDECANOIC ACID
DI-N-BUTYLDIPHENYLTIN
4-Chloro-3-Fluorobenzaldehyde
Ethyl 3-broMoiMidazo[1,2-a]pyridine-6-carboxylate, 95%
(R)-(-)-1,1'-Binaphthyl-2,2'-diyl hydrogen phosphate, 98+%
2-Chloro-3',4'-dihydroxyacetophenone, 97%
10-BroModecanoic acid
8-BroMo-6-Methyl-3-nitroiMidazo[1,2-a]pyridine
2-BroMo-6-phenylpyridine
COPPER(II) SULFATE HYDRATE, 98%
2-BroMo-5-Methoxybenzyl broMide
2-Chloro-6(MethylaMino)purine
Benzoyl chloride
2-(3,4-Dihydroxyphenyl)ethyl Alcohol
(S)-beta-(3-fluoro-phenyl)alanine