SP2/o Cell：小鼠杂交瘤复苏贴壁细胞系 货号：GD-C6419298
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
6-Chloro-4-(furfurylaMino)pyriMidine, 96%
2-Iodopropane
α-(4-TolyliMino)-p-cresol
(3-ACETYLPHENOXY)ACETIC ACID
Ethyl 2-aMino-4-thiazoleacetate
Monoisopropyl FuMarate , 97%
4,4,4-TRICHLOROACETOACETIC ACID ETHYL ESTER
Gallium(III) oxide (99.998%-Ga) PURATREM
1,3-Phenylene-bis(2-thiourea)
Girard's Reagent P
2-Methyl-1-naphthyl Acetate
ETHYL 2,4-DIMETHYLTHIAZOLE-5-CARBOXYLATE
Cyclobutane-1,2,3,4-tetracarboxylic dianhydride
2-HYDRAZINOBENZOTHIAZOLE
Bis(triMethylsilyl)sulfide
Methanol-d
(-)-(5aS,10bR)-5a,10b-Dihydro-2-(2,4,6-triMethylphenyl)-4H,6H-indeno[2,1-b][1,2,4]triazolo[4,3-d][1,4]oxaziniuM Chloride Monohydrate
5-BroMo-1H-indazole-3-carbaldehyde
4-(2-Methyl-1H-iMidazol-1-yl)benzoic acid hydrochloride hydrate, 95%
TERT-BUTYL ALCOHOL
4-(5-Chloro-2-pyridylazo)-1,3-phenylenediaMine [for ColoriMetric Analysis of Co, Cd]
trichloro-p-tolylsilane
2-Chloro-5-nitrobenzaldehyde
methoxy-bis(dimethylamino)methane
4-Ketopimelic acid
N-(2-BROMOBENZYL)ACETAMIDE
4-Aminotoluene-3-sulfonic acid
N,N-Diphenylbenzidine
Gold(I) sodiuM thiosulfate hydrate, 99.9% (Metals basis)
4-Iodophenol, 98+%
Benzothiophene-2-Methanol
D-(-)-Tartaric acid
3,5-Difluoroanisole
N-t-Butyl 3-broMo-4-MethylbenzenesulfonaMide
2-Boc-AMino-4-chloropyridine
5-TERT-BUTYL-2-METHYLTHIOPHENOL
Montelukast acyl-β-D-glucuronide ,98%
1,3-Benzodioxol-5-ylacetyl chloride 97%
4-Nitrophenyl β-lactoside heptaacetate ,90%
Potassium triethylborohydride, 1M solution in THF, AcroSeal
Ethyl propionylacetate
Sodium 2-amino-1,4-benzenedisulfonate
1-AMINOHEPTADECANE
Thiamine hydrochloride
1-AMinopyrene
5,5'-DibroMo-2,2'-bithiophene
3-Methoxy-4-Methylbenzonitrile
N-(5-Fluoro-2-Methoxyphenyl)acetaMide
Chlorocyclopentadienylbis(triphenylphosphine)rutheniuM(II), 98%
Mebeverine hydrochloride
Hexadecyl Ether
Pigment Red 52:1
1-(6-methylpyridin-3-yl)ethan-1-one
2-(trifluoromethyl)thiophenol
4-Methyl-3-nitropyridine
Heptaldehyde
4-[4-Chloro-3-(trifluoroMethyl)phenyl]-4-piperidinol
POTASSIUM TETRABROMOPLATINATE(II)
3,5-DINITRO-L-TYROSINE SODIUM SALT
Diisopropyl Disulfide
Aminoacetonitrile Hydrogen Sulfate
4-Penten-1-ol
6,7-Dimethoxy-3-methyl-1,2,3,4-tetrahydroisoquinoline hydrochloride, 99%
Valeronitrile
Diethylaminoethanol
N-Methyliminodiacetic acid
Diaminomaleonitrile
3,4-Difluorobenzyl broMide
1-Butyl-4-MethylpyridiniuM BroMide
2-Cyano-1,5-diMethylpyrrole-3-boronic acid, pinacol ester
Chloroacetic acid sodiuM salt, 98%
3,5-Bis(trifluoroMethyl)benzylaMine, 95%
Indanthrene Black BBN
2-Methyl-1,4-pentadiene
2-Azetidinone
4-chloro-7-fluoroquinoline-3-carbonitrile
SERMORELIN ACETATE
( Z )-2-(2-Aminothiazol-4-yl)-2-(t-butoxycarbonylmethoxyimino)acetic acid
CYPA ELISA Kit
PL12/AlaRS ELISA Kit
Human AMELY ELISA Kit
Human dihydropyrimidinase-like 3,DPYSL3 ELISA Kit
AF ELISA Kit
β-Cat ELISA Kit
Chicken IgY (Immunoglobulin Y) ELISA Kit
Human POMT2 ELISA Kit
Human thrombus precursor protein,TpP ELISA Kit
PCDHβ2 ELISA Kit
Ammonium Dextrose Medium
Gelatin Medium
ScandiuM(III) 2,4-pentanedionate hydrate
VanadiuM(III) fluoride, 98%
5-(Boc-aMino)-2-chlorobenzoic acid, 98+%
2-(4-BROMOPHENOXY)TETRAHYDRO-2H-PYRAN
trichloro-p-tolylsilane
2,4-Difluorobenzenesulfonamide
Silver oxide
Phenylacetylene
2,4-Dimethoxybenzeneboronic acid
4-Cyanoindole
Allyltri-n-Butyltin
2,3,4,6-Tetrafluorobenzoyl chloride, 98%
L-3-(FMoc-aMino)-N-trityladipic acid 6-aMide, 95%
PalladiuM(II) trifluoroacetate
α,α-Difluorophenylacetic acid
2,3-DIMETHOXYTOLUENE
4-BroMo-2,5-diMethoxybenzaldehyde
1,2-Decanediol
Methyl 4-thiocyanatobutanoate
Deuterium Oxide BIS001E
2-Cyanoethyltriethoxysilane
Cefpirome Sulfate
Cerium(III) perchlorate hexahydrate, reagent
