HT22 Cell：小鼠海马神经元复苏贴壁细胞系

HT22 Cell：小鼠海马神经元复苏贴壁细胞系 货号：GD-C6418867

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1-(2-BroMophenylsulfonyl)piperidine, 97%
H-β-HoMoglutaMic acid hydrochloride
2-Benzoxazolinone
2,2′-DibroMobenzophenone
4-AMinobenzoic acid potassiuM salt
2,6-DIMETHYL-3-HEPTENE
3-CHLORO-1-BUTYNE
5-(2-Pyridyl)thiophene-2-carboxylic acid, 97%
Allyl iodide
4-Nitroguaiacol, 97%
1-Fluorocyclohexane-1-carboxylic acid
Perrhenic acid
2,6-DIFLUORO-3-METHYLBENZOYL CHLORIDE
Quinoline-5-boronic acid
2,5-Dibromonitrobenzene
4-Ethoxy-3-Methoxybenzyl alcohol
3,7-Dithia-1,9-nonanedithiol
4-Chloro-6-(4-iodo-1H-pyrazol-1-yl)pyriMidine
TetraMethyltin, 98%
Nortriptyline Hydrochloride, USP
2-Heptenoic Acid (contains 3-Heptenoic Acid)
Octaphenylcyclotetrasiloxane
5-Bromo-3-methoxysalicylaldehyde
Cyclobutanecarboxylic acid methyl ester
2-hydroxybiphenyl sodium salt
(S)-4-Fluorophenylglycine
N-DODECYL-BETA-D-GLUCOPYRANOSIDE
Acetylpyrazine
1-n-ButylpyridiniuM chloride, 98%
2,2,3,3,3-PentafluoropropionaMide, 97%
3-[2-(Ethoxycarbonyl)ethyl]benzeneboronic acid, 97%
4-Chloro-3-(trifluoroMethyl)benzylaMine
1-Boc-4-(2-aMinoethyl)
SILICON TETRAISOTHIOCYANATE
1,2-BIS[2'-(TRIFLUOROMETHYL)PHENYL]ETHANE
3-Methylpyridine-2-carboxylic acid
2'-Benzoyl-2,4'-dichloroacetanilide
1-tert-Butyloxycarbonyl-3-bromo-4-oxopiperidine
[3-(cyclopropylcarbonyl)-1H-indol-1-yl]acetic acid(SALTDATA: FREE)
4-Amino-3,5-diiodobenzoic acid, 85%
4-[(Fur-2-ylmethyl)carbamoyl]benzeneboronic acid 97%
Poly(1,2-dihydro-2,2,4-trimethylquinoline)
Zinc citrate
3,5-Difluorophenylhydrazine hydrochloride
(R)-3-Amino-3-phenylpropan-1-ol
5-Methyl-1H-pyrazole-3-carboxylic acid
cis-2,2-DiMethyl-3-hexene
2-BroMo-5-Methoxypyridine
PotassiuM hexachloroplatinate(IV), ca. 40% Pt
gaMMa-Cyclodextrin
4-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)phenol
Tetramethylthiuram disulfide
docosanamide
6-Aminobenzothiazole
Acrylamide
2-Bromo-4-fluoroaniline
ETHYL HYDROGEN SUBERATE
10,12-HEPTADECADIYNOIC ACID
2-(Cbz-AMino)-5-Methylpyrazine
6-Fluoro-1H-indole-2-carboxylic acid
1,3-Dichlorotetraphenyldisiloxane
1,2-Bis(dimethylsilyl)benzene
3,4'-Dinitrobenzophenone
2,4-Diaminobenzenesulfonic acid
1-(2,4-DICHLORO-PHENYL)-ETHYLAMINE
N-Benzyl-4-piperidone
POLY(4-VINYLPHENOL)
N-(5-Anilino-2,4-pentadienylidene)aniline hydrochloride
1,2-xylene
4,4,4-Trifluoro-1-(p-tolyl)-1,3-butanedione
3,4,5-TriMethoxyphenylacetic acid, 99%
3-(3-Isocyanatophenyl)-5-Methyl-1,2,4-oxadiazole, 97%
Ethyl cellulose
Cholesterol Methyl Carbonate
trifluoromethane
Ascorbic acid 2-phosphate magnesium ester
2-Bromo-4-t-octylphenol
1-bromocyclobutanecarboxylic acid
CDH5 ELISA Kit
δLK1 ELISA Kit
Human FADS2 ELISA Kit
Human CAPN1 ELISA Kit
Chicken AR (Amphiregulin) ELISA Kit
Rat HHIP (Hedgehog Interacting Protein) ELISA Kit
Monkey ADIPOR1 (Adiponectin Receptor 1) ELISA Kit
FGFBP1 ELISA Kit
Human Thymosin α1 ELISA Kit
HABP ELISA Kit
Sabouraud Dextrose Broth
BAT Broth
Indole-5-boronic acid, 95%
Allyl heptanoate, 97%
5-AMINO-1H-TETRAZOLE
3-Methyl butynol
Octaphenylcyclotetrasiloxane
Stachyose tetrahydrate
3-Chloro-2-methylbenzoic acid
4-Aminobenzoic acid

L-4-Chlorophenylalanine
1H,1H-PERFLUOROUNDECAN-1-OL
5-BroMo-1,3-Difluoro-2-Methoxybenzene
Ethyl nonanoate, 97%
4-BroMobenzotrifluoride, 99%
MagnesiuM broMide
HolMiuM oxide
4-METHYLTHIAZOLE-2-THIOL
3-ACETOXY-2-BUTANONE
Methyl cyanoacetate
2-(5-BROMO-2-PYRIDYLAZO)-5-(DIETHYLAMINO)PHENOL
3-(Methylsulphonyl)aniline
Fmoc-4-chloro-L-phenylalanine
trans-2,3-Difluorocinnamic acid
(S)-2-Hydroxybutyl p-tosylate, 98+%