MOVAS Cell:小鼠主动脉血管平滑肌复苏贴壁细胞系 货号:GD-C6418734
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作):1)吸出原培养瓶中的培养基,PBS缓冲液润洗细胞两次,加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间,通常控制在1-2min);2)镜下观察消化情况,在细胞边缘缩小,贴壁松动时(不建议消化到细胞漂浮)去掉胰酶,加6-8ml完全培养基,轻轻吹打细胞层,尽量把细胞层吹落,吹散;3)取部分细胞悬液转移到新的培养皿/瓶中,添加适当的完全培养基,把细胞悬液打匀,于培养箱中培养;4)注意培养基PH值变化情况,定期换液(每周2-3次),待细胞密度达到80%以后重复1项操作或者冻存;特别注意:(如使用公共实验室或者初次接触细胞培养,建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基,如因特殊情况需要继续使用原瓶,请在原瓶培养基中额外添加10%的血清,(原瓶培养基的继续使用时间Z长不宜超过72小时);2)贴壁细胞收到当天切忌立刻消化,请将细胞换液后放置培养箱孵育到第二天再做消化传代,请优先选择直径6cm的培养皿进行传代培养;3)如签收时出现培养瓶壁破裂,漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结:1)一般客户拿到细胞后,应该注意什么?客户收到细胞后先不开盖,放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况,并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况,显微镜下拍细胞100X,200X各一张),排除细胞本身污染的情况;收到细胞未开封,出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况,请在显微镜下观察细胞密度,如为贴壁细胞,未超过80%汇合度时,将培养瓶中培养液吸出,留下10ml培养液继续培养;超过80%汇合度时,请按细胞培养条件传代培养。如为悬浮细胞,吸出培养液、1000转/分钟离心2分钟,吸出上清,管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制,慎用Hanks液配制;2)快递细胞多久能到,是寄冻存的细胞还是复苏好的细胞?我们采用快递发货,一般外地2--3天,寄细胞前请确认当地温度,如果气温低于4度的,则采用邮寄冻存细胞;3)可否使用与原先培养条件不同之培养基?不能。每一细胞株均有其特定使用且已适应之细胞培养基,若骤然使用和原先提供之培养条件不同之培养基,细胞大都无法立即适应,造成细胞无法存活;4)可否使用与原先培养条件不同之血清种类?不能。血清是细胞培养上一个极为重要的营养来源,所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清,在一些物质或分子的量或内容物上都有所不同,血清使用错误常会造成细胞无法存活。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-BroMo-N-(2,4-difluorophenyl)benzenesulfonaMide, 97%
3-AMino-4-Methoxybenzanilide
Hyaluronidase froM Bovine Testes
Ethyl 3,5-diMethyl-1H-pyrrole-2-carboxylate
Ethyl diethoxyphosphinylforMate
4-BroMo-6-chloropyridine-3-boronic acid
Methyl (3R)-(?)-3-(1-Methylindol-3-yl)butanoate
3,4-Dimethoxythiobenzamide
2-Acrylamido-2-methylpropanesulfonic acid, 97%
trans-3-Methyl-2-pentene
6-Bromo-4-chloro-thieno[3,2-d]pyrimidine
BENZYLHYDRAZINE MONOHYDROCHLORIDE
4-Hydroxybenzenesulfonic acid
5-Fluorosalicylic acid
N,N-Diethylchloroacetamide
Ethyl 1-(2-furylMethyl)-4,5-dioxopyrrolidine-3-carboxylate
5,6-Dihydro-1,4-dithiin-2,3-dicarboxiMide
6-BroMo-1h-pyrrolo[3,2-b]pyridine
syM-DiMethylhydrazine dihydrochloride, 99+%
Methyl Red SodiuM Salt, Reagent, ACS
Glycylglycine Hydrochloride Monohydrate
Tetraphenylarsonium chloride
1,2-dichloro-1,1,2,2-tetramethyldisilane
n-Pentadecane
pyrimidine-4,5,6-triyltriamine
2,3-DIFLUOROBENZOIC ACID
(+)-Dimethyl L-tartrate
1-ButyliMidazole
2,4-DiMethylphenoxyacetic acid, 98+%
4-n-Propoxyaniline, 97%
1,2-DiaMinocyclohexane, Mixture of cis and trans
IMidan
Cyclopropyl 4-fluorophenyl ketone
TantaluM(V) Methoxide
DIMETHYLZINC
4-Propylpyridine
Alloxantin
2-(Aminomethyl)benzoic acid
2-Bromo-3-fluoronitrobenzene
Barium(II) oxide 99.9%
2-Aminobenzotrifluoride
N-Ethylbenzylamine
(R)-3-AMINO-4-(3-METHYLPHENYL)BUTANOIC ACID HYDROCHLORIDE
CysteaMine;Decarboxycysteine;2-AMinoethanethiol;2-AMinoethylMercaptan;β-MercaptoethylaMine;ThioethanolaMine;MercaMine; MEA
Methyl oleate
TBTU
n-Octyl Gallate
3-AMino-1,2,3,4-tetrahydrocarbazol
Ethylene glycol diethyl ether, 99%
Thalidomide
cis-2-Hexen-1-ol
2-Ethyl-6-methyl-3-hydroxypyridine
Germacr-1(10)-ene-5,8-dione
3,5-Dibromosalicylic acid
biphenyl-2,5-diol
PYRAZOLO[1,5-A]PYRIDINE-7-CARBALDEHYDE
Ethyl 3-(2-triMethylacetaMido-3-pyridyl)acrylate
4-(METHYLTHIO)PHENYL ISOCYANATE
trans-DiaMMinedichloropalladiuM(II)
2-Amino-6,7,8,9-tetrahydrodibenzo[b,d]furan 97%
8-Amino-1-octanol
5,6,7,8-Tetrahydronaphthalene-1-carboxylic Acid
(+)-Terpinen-4-ol
5-CYANO-2-FLUOROBENZALDEHYDE
Sodium sulfadiazine
Isocyanatocyclohexane
TRIETHANOLAMINE BORATE
2,6-Dichloroindophenol
1-(CarbaMoylMethyl)pyridiniuM Chloride
3-Methoxypyridine-2-carbonitrile
3-DiMethylaMinobenzoic acid, 99%
2-Methyl-1,3-cyclopentanedione, 98%
LIGNOCERIC ACID
Diethyl Suberate
4-Nitrophenylhydrazine
(R)-3-(1-aminoethyl)benzonitrile
Iron(III) trifluoromethanesulfonate
Metoprolol
PDGFD ELISA Kit
IL-1sRⅠ ELISA Kit
Human NSMASE ELISA Kit
Human Mannma binding protein/mannan binding lectin,MBP/MBL ELISA Kit
T ELISA Kit
CER ELISA Kit
C3b ELISA Kit
Human LAMα2 ELISA Kit
Human Neopterin ELISA Kit
GHITM ELISA Kit
YSG Agar Medium Base
Lactobacillus Selective Broth
D-Pantothenic acid calciuM salt hydrate, 98%
Decanaldehyde
5-Fluorouracil, 99%
2-Methylcyclohexanol
Tetraphenylarsonium chloride
Ammonium sulfate
3-Bromoimidazo[1,2-a]pyrimidine
3,4-Dimethoxybenzaldehyde
1,1,1-Trimethoxypentane
1-Hexene
3,4-Diaminofluorobenzene
2-(DiMethylcarbaMoyl)benzoic acid, 97%
2-Mercapto-5-nitrobenziMidazole, 97%
Methyl benzoate
Allyl cyanide
BENZYL 6-BROMOHEXYL ETHER
D-Serine benzyl ester benzenesulfonate
2-AMino-4-(iMino(Methoxy)Methyl)phenylboronic acid, HCl
3,4-Difluorophenylhydrazine hydrochloride, 98%
3-Thienylmethylamine, 97%
3-Methoxypyridine
2-Hydroxyisonicotinonitrile
Palmitic Acid Hydrazide