BSMSCs Cell:牛骨骼肌卫星复苏贴壁细胞系 货号:GD-C6418701
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作):1)吸出原培养瓶中的培养基,PBS缓冲液润洗细胞两次,加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间,通常控制在1-2min);2)镜下观察消化情况,在细胞边缘缩小,贴壁松动时(不建议消化到细胞漂浮)去掉胰酶,加6-8ml完全培养基,轻轻吹打细胞层,尽量把细胞层吹落,吹散;3)取部分细胞悬液转移到新的培养皿/瓶中,添加适当的完全培养基,把细胞悬液打匀,于培养箱中培养;4)注意培养基PH值变化情况,定期换液(每周2-3次),待细胞密度达到80%以后重复1项操作或者冻存;特别注意:(如使用公共实验室或者初次接触细胞培养,建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基,如因特殊情况需要继续使用原瓶,请在原瓶培养基中额外添加10%的血清,(原瓶培养基的继续使用时间Z长不宜超过72小时);2)贴壁细胞收到当天切忌立刻消化,请将细胞换液后放置培养箱孵育到第二天再做消化传代,请优先选择直径6cm的培养皿进行传代培养;3)如签收时出现培养瓶壁破裂,漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结:1)一般客户拿到细胞后,应该注意什么?客户收到细胞后先不开盖,放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况,并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况,显微镜下拍细胞100X,200X各一张),排除细胞本身污染的情况;收到细胞未开封,出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况,请在显微镜下观察细胞密度,如为贴壁细胞,未超过80%汇合度时,将培养瓶中培养液吸出,留下10ml培养液继续培养;超过80%汇合度时,请按细胞培养条件传代培养。如为悬浮细胞,吸出培养液、1000转/分钟离心2分钟,吸出上清,管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制,慎用Hanks液配制;2)快递细胞多久能到,是寄冻存的细胞还是复苏好的细胞?我们采用快递发货,一般外地2--3天,寄细胞前请确认当地温度,如果气温低于4度的,则采用邮寄冻存细胞;3)可否使用与原先培养条件不同之培养基?不能。每一细胞株均有其特定使用且已适应之细胞培养基,若骤然使用和原先提供之培养条件不同之培养基,细胞大都无法立即适应,造成细胞无法存活;4)可否使用与原先培养条件不同之血清种类?不能。血清是细胞培养上一个极为重要的营养来源,所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清,在一些物质或分子的量或内容物上都有所不同,血清使用错误常会造成细胞无法存活。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Benzoic anhydride, 98%
4,5-Dichloro-o-phenylenediaMine
(1R,3R)-5-(BROMOMETHYLENE)-1,3-BIS(TERT-BUTYLDIMETHYLSILYLOXY)CYCLOHEXANE
METHYL 2-ACETAMIDOBENZOATE
5-Fluoroindole-2-carboxylic acid
YTTRIUM ALUMINUM OXIDE
4-Hydrazinobenzene-1-sulfonaMide hydrochloride
Dodecanedioyl dichloride, 98%
Ethyl 4-(4,4-dimethylpiperidino)benzenecarboxylate
9,10-Dimethylanthracene, 97%
Aceanthrenequinone, 95%
4-Hydroxy-2-methoxybenzaldehyde
Undecanoyl chloride
1-(4-AMINOPHENYL)-4-(4-METHOXYPHENYL)
2-CHLORO-5-METHYLBENZOIC ACID
1-Pentanesulfonic acid
S-(N,N-DiMethylthiocarbaMoyl)thioglycolic Acid
3-BroMopyridazine
SodiuM hydride, 60% dispersion in Mineral oil, in soluble bags
Mepivacaine Hydrochloride, USP
Glycyl-DL-valine
Propiolic acid
4-Pyrimidinamine,6-chloro-5-nitro-
hept-1-yne
N,N,N',N'-Tetramethylethylenediamine
Amylamine
Di-n-butyl ether
Proflavine HeMisulfate
TetraphenylphosphoniuM iodide, 98+%
Triphenylphosphine hydrobroMide, 97%
1,3,5-Benzenetricarbonyl trichloride
(±)-Epichlorohydrin
AMMoniuM Molybdate tetrahydrate
TERT-BUTYL 4-(5-IODOPYRID-2-YL)-1-CARBOXYLATE
2-Mercapto-5-Methyl-1,3,4-thiadiazole
7-HYDROXYCOUMARIN-4-ACETIC ACID
2-Ethyl-1,3-hexanediol (mixture of diastereoisomers)
6-Chloropyridine-2-sulphonic acid
5-Methyl-2-phenyl-2-hexenal
1-amino-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamide(SALTDATA: FREE)
(S)-ALPHA-METHYL-4-NITROBENZYLAMINE HYDROCHLORIDE
6-CYANONICOTINIC ACID
5,6-Difluoro-2-oxoindole
KANAMYCIN SULFATE
Methyl pyridine-2-carboxylate
N-Acetyl-L-tyrosine Ethyl Ester Monohydrate
1-(3-Nitrophenyl)pyrrolidin-2-one
3,5-DiMethoxybenzyl alcohol, 98%
5-(2-Furyl)-1H-pyrazole-3-carboxylic acid, 97%
TRIHEPTADECANOIN
(R)-4-(4-Methoxybenzyloxy)-1,2-butanediol
1-Aminonaphtalene-5-sulfonic acid
Ceftibuten dihydrate
methyl 3-bromopyridine-4-carboxylate
(6-Chloro-2-pyridinyl)methanol
(4-Pyridylthio)acetic acid
ALPHA-METHYL-GAMMA-BUTYROLACTONE
Graphite
2-AMino-4-ethoxycarbonylphenylboronic acid, HCl
L-Prolinol ,98%
7-Methoxy-3-phenylsulfonyl-1(3H)-isobenzofuranone
Chlorodifluoroacetyl chloride
Direct Yellow 44
D-Xylose
ETHYL ALLOPHANATE
ALPHA-PHENYLCYCLOPENTANEACETIC ACID
RHENIUM (VI) OXIDE
2-AMino-3-iodopyridine
1-(3-AcetaMidophenyl)-5-Mercaptotetrazole
2,4-Dichloro-3-Methylpyridine
4-(4-Nitrophenylazo)resorcinol, 90%
1H-BenziMidazol-5-ylMethanol, 95%
alpha-D-Methylglucoside
eta-Benzene(eta-cyclopentadienyl)iron(II) Hexafluorophosphate
1-Bromotetradecane
(S)-2-(Boc-amino)-2-phenylethylamine
2-Oxa-1,4-butanediol diacetate
(Z)-Dodec-5-enol
Porcine nitric oxide,NO ELISA Kit
IL-21 ELISA Kit
Human TNF-β ELISA Kit
Human TG ELISA Kit
GAL14 ELISA Kit
CD34 ELISA Kit
Monkey IL-12 (Interleukin 12) ELISA Kit
Human Opacity-associated proteins,OPAs ELISA Kit
Human atrial natriuretic factor,ANF ELISA Kit
NT-2R ELISA Kit
Shigella Broth Base
Bacillus Medium Base
1,1,1-TriMethoxypropane
SodiuM Persulfate
5-Hexynoic acid, 97%
5-BroMo-2-(Methylthio)PyriMidine-4-Carboxylic Acid ,97%
Propiolic acid
Zinc nitrate
1,3-Disilabutane
2,4-Dinitroanisole
FERROUS GLUCONATE DIHYDRATE
3-Mercaptopropionic Acid
9-FluorenylMethyl chloroforMate, 98%
Triethyl citrate, 99%
1,3-Diphenylisobenzofuran
(1E)-1-Methoxy-2-Methyl-1-penten-3-one
2-Pentylfuran
GADOLINIUM BROMIDE
Methyl 6-pyrrolidinonicotinate
DL-Panthenol
Ethyl 2-(Bromomethyl)acrylate (stabilized with HQ)
Tetramethylene-d8 sulfone, 99+ atom% D
Sodium DL-2-Hydroxybutyrate