HPASMC Cell:人肺动脉平滑肌复苏贴壁细胞系 货号:GD-C6418622
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项:1)如果细胞为贴壁细胞,而收到时呈悬浮或者部分悬浮的状态,请将悬浮的细胞即时离心,加15%血清的完全培养基到新的培养皿/瓶继续培养3天;同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡,请及时联系实验室技术人员会跟进解决;2)贴壁细胞生长缓慢;适当提高血清浓度(不能超过20%),或可根据该细胞生长密度,考虑胰酶消化后,转移到新的培养瓶继续培养;3)生长不均:贴壁细胞若出现分布不均,成岛状生长,可将细胞进行消化,重悬打散细胞,加入新鲜培养基进行培养。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3,4-DiMethoxyphenylacetic acid, 98%
4-Chloro-3-fluorotoluene
4-CYANOPHENYL ETHER
6,7-Dichloro-3-trifluoroMethylquinoxaline-2-carboxylic acid
EXO-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC ANHYDRIDE
3-Hydroxy-4-MethoxybenzylaMine hydrochloride
4-MERCAPTOHYDROCINNAMIC ACID
4-(2-Bromoacetyl)benzenesulfonyl chloride
Pyridinium tribromide
Boc-D-Nle-OH
Fmoc-3,4-dimethoxy-L-phenylalanine
Chlorophenol Red
1,1-DIPHENYL-2-PROPYN-1-OL
Tri-tert-butylphosphine
5-Amino-4,6-dichloropyrimidine
N,N'-Bis(2-hydroxyethyl)ethylenediaMine
2,2-DibroMo-1,3-diphenyl-1,3-propanedione
3-(4-BroMophenyl)-1H-pyrazole-5-carboxylic acid
SodiuM Metabisulfite, 97+%, ACS reagent
L-Thiocitrulline
6-Methyl-1,2,3,4-tetrahydroquinoline
N(alpha),N(epsilon)-di-Z-L-lysine
2-Bromobenzamide
Benzylmalonic acid
4-butylaniline
4-Fluorobenzyl alcohol
L-Phenylglycine
2-Isopropylphenylboronic Acid
2,2-DiMethyl-3-octanol, 98%
1-Cyclopropyl-4-piperidone, 98%
2-(Dicyclohexylphosphino)biphenyl
Propyl 2-Methyl-3-furyl disulfide
5-BROMO-2-FLUOROBENZYLAMINE HYDROCHLORIDE
TETRAKIS(DIMETHYLSILOXY)SILANE
Methyl 2-Methoxynicotinate
2,2'-Thiodi(4-tert-octylphenol)
3-(N,N-Diethylsulphamoyl)benzeneboronic acid 98%
Methyl Gallate
H-Ser(tBu)-OH
Benzophenone-2,4,5-tricarboxylic Acid
2-ANTHRACENECARBOXYLIC ACID
Ethyl 4-bromobutyrate
1H,1H-PERFLUORO-1-DODECANOL
MALONALDEHYDE DIANILIDE HYDROCHLORIDE
4,5-DichloropyriMidine
D-Tryptophan
2,3,5,6-Tetrachloropyridine
2-Furoyl chloride, 98+%
7-Hydroxyflavanone, 98%
Trisodium phosphate dodecahydrate
3-AMino-9-ethylcarbazole [for BiocheMical Research]
1,6-BIS(P-ACETOXYCARBONYLPHENOXY)HEXANE&
5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-deoxycytidine
2,2-Dimethyltetrahydro-2H-pyran-4-carboxylic acid
P,P'-dimethyl dihydrogen diphosphate
4-Chlorobenzenesulfonyl isocyanate
L-Talitol
MOLYBDENUM SELENIDE
4,4-Oxybis(1,4-phenylene)diboronic acid
Quincorine
1,3-Cyclohexanedione [for HPLC Labeling]
Fmoc-2-Abz-OH
3-Methyl-2-hexanone
2',4',5'-TRIMETHYLACETOPHENONE
4-PYRROLIDINOBUTYLAMINE
Bis(8-quinolinolato) zinc
TRIS BORATE EDTA BUFFER, 10X, DNASE, RNASE AND PROTEASE FREE, PH 8.3, FOR MOLECULAR BIOLOGY
Benzylacetone
(S)-4-Benzyloxy-1,2-butanediol
3-(Cyclopentyloxy)-4-Methoxyphenylboronic acid
cis-1,4-Dichloro-2-butene, 95%
Ethyl 4-(trifluoroMethyl)pyriMidine-5-carboxylate, 95%
1-Naphthol
Acid Orange 5
sodium 2,4-dinitrophenoxide
1H-Pyrazole-4-carboxylicacid, 1-phenyl-3-(trifluoromethyl)-, ethyl ester
N(alpha)-boc-N(delta)-(2-chloro-Z)-L-ornithine
ethyl pipecolinate hydrochloride
ADH1 ELISA Kit
IL-5 ELISA Kit
Human NE-B ELISA Kit
L.FABP ELISA Kit
GSH-PX ELISA Kit
BAX ELISA Kit
Cath-S ELISA Kit
Human C5 ELISA Kit
ALPI ELISA Kit
NA ELISA Kit
Lactobacillus Selective Agar
LB Broth,Lennox
Piperonyl butoxide
Black Pepper Oil
5-Methyluridine, 99%
2-Methoxypyridin-3-aMine
N(alpha),N(epsilon)-di-Z-L-lysine
2,4-Dihydroxy-6-Phenyl-1,3,5-Triazine
4-chloro-5-methyl-o-anisidine
N-Methylethylenediamine
(R)-(-)-1,3-Butanediol
1,2-EPOXYHEXADECANE
5-Norbornene-2,3-DicarboxiMide
3-(Boc-aMino)piperidine, 97%
Methyl 2-aMino-4-chlorobenzoate, 98%
IndiuM(III) nitride
Titan yellow
(-)-BenzylMethylphenylsilylacetic Acid [for e.e. DeterMination by NMR], 98%
5-Norbornene-2,3-diMethanol , 95%
Disperse Violet 28
1-(4-METHYLPHENYL)ETHYLAMINE 96
Potassium Tri-sec-butylborohydride (ca. 1.0mol/L in Tetrahydrofuran)
Methyl 2,4-difluorobenzoate
DL-Norvaline
3,5,5-Trimethyl-2-cyclohexen-1-ol ,95%