DiFi Cell：人结直肠癌复苏贴壁细胞系

DiFi Cell：人结直肠癌复苏贴壁细胞系 货号：GD-C6418530

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项：1)如果细胞为贴壁细胞，而收到时呈悬浮或者部分悬浮的状态，请将悬浮的细胞即时离心，加15%血清的完全培养基到新的培养皿/瓶继续培养3天；同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡，请及时联系实验室技术人员会跟进解决；2)贴壁细胞生长缓慢；适当提高血清浓度(不能超过20%)，或可根据该细胞生长密度，考虑胰酶消化后，转移到新的培养瓶继续培养；3)生长不均：贴壁细胞若出现分布不均，成岛状生长，可将细胞进行消化，重悬打散细胞，加入新鲜培养基进行培养。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Decanone, 99%
SiMazine
5,6-DIHYDRO-2H-PYRAN-2-ONE
2,3,4,5,6-Pentafluorobenzyl alcohol
2,3-Pyrazinecarboxylic anhydride
4-(TrifluoroMethoxy)benzyl broMide
1-Methyl L-aspartate
4-Methylsulphonylacetophenone, 95%
Phenyl trans-styryl sulphone
1H-Imidazol-4-ylmethylamine dihydrochloride
Dimethyl 5-nitroisophthalate 98+%
2,4-Dichlorobenzonitrile
2-Fluoro-5-formylphenylboronic acid
2-Nitrofluorene
2-Chloro-4-iodoaniline
Florisil
3,3'-Difluorobiphenyl
2-BroMo-N-pentylbenzenesulfonaMide
S-CarboxyMethyl-L-cysteine, 98%
L-Cystine Dihydrochloride
4-BroMo-2,5-dichlorophenol
Alpha-Fluorocinnamic acid
disodium N-(2-carboxyethyl)-N-dodecyl-β-alaninate
N-Methylthiourea
2-Methylpentanoic acid
CYCLOPENTADECANE
2-TETRAHYDROFURFURYL ISOTHIOCYANATE
1,2-Dichlorobenzene
2-Hydrazino-4-(trifluoroMethyl)pyriMidine, 97%
Benzylzinc broMide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
2-Methyl-5-(trifluoroMethyl)benzaldehyde
3-Nitrophenylboronic acid
1,2-Bis[(2R,5R)-2,5-diMethylphospholano]benzene Monooxide
trans-2,3,4-TriMethoxycinnaMic acid
SAMARIUM CARBONATE
DL-Lactide
3-Methylpentane, 99+%
Phenylethyl β-D-galactopyranoside
2-Chloroallyl alcohol 98%
1,2-Dibromo-1,1,2-trichloroethane, 99%
GADOLINIUM FLUORIDE
ZINC CARBONATE BASIC
Sodium malate
N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE
Methyl isocyanoacetate
Tetrahydro-2,2-diMethyl-4H-pyran-4-one
Butyl TrifluoroMethanesulfonate
2-Cyanophenylboronic acid, 98%
4-Isopropoxyphenylboronic acid, 97%
1,6-DIMETHYLNAPHTHALENE
Methyl Diphenylacetate
1-(2-FLUORO-PHENYL)-BUTANE-1,3-DIONE
2-(4-thiazolyl)benzimidazole
3-phenoxyphenylacetonitrile
3-{3,3,3-trimethyl-1,1-bis[(trimethylsilyl)oxy]disiloxanyl}propan-1-amine
Titanium ethoxide
ChloroforM-13C
Ethyl 4-(ButylaMino)benzoate , 98%
8-Hydroxyquinoline sulfate Monohydrate
1-Naphthyl isocyanate
N,N'-Dinitrosopentamethylenetetramine
6-Piperidin-1-ylnicotinaldehyde , 97%
Methyl 2-(2-{[(benzyloxy)carbonyl]amino}propan-2-yl)-5,6-dihydroxypyrimidine-4-carboxylate
4-BIPHENYLSULFONIC ACID
2-CHLOROCYCLOHEXANOL
4-BROMO-3-(TRIFLUOROMETHYL)ACETANILIDE
4-Nitrobenzyl chloroformate
1-Iodobutane (stabilized with Copper chip)
(R)-1-Boc-3-hydroxypiperidine
Monocaprin
2,3-DiaMinobenzoic acid
DiMethylphenylsilane, 98%
Amylcinnamaldehyde
Biphenyl-4-carboxylic Acid
4-Cyanobenzylamine
Glipizide
Tween(R) 60
Disiloxane, 1,3-diethynyl-1,1,3,3-tetramethyl-
APOA2 ELISA Kit
IL-6 ELISA Kit
Human TEKT5 ELISA Kit
Human GLG1 ELISA Kit
MPO ELISA Kit
PⅠNP ELISA Kit
OSMR ELISA Kit
PDHE1 ELISA Kit
CKMT1A ELISA Kit
ODF ELISA Kit
Elek Medium
Phenylethyl Alcohol Agar Base
SodiuM 1-butanesulfonate, 99%
Ancymidol
5-Norbornene-2,3-dicarboxylic acid
2-Hydrazinobenzoic acid hydrochloride
Alpha-Fluorocinnamic acid
ethyl 3-oxoicosanoate
2-Chloro-4-methylquinazoline
Phenethyl acetate

4,4'-Diacetylbiphenyl
4-(Bromomethyl)phenylboronic acid
Ethyl Phenylacetate
1-Benzyloxycarbonyl-4-piperidone, 98+%
VinyloxytriMethylsilane, 97%
L-Methionine Methyl Ester Hydrochloride
RuCl(p-cyMene)[(S,S)-Ts-DPEN]
Butyraldehyde oxiMe
2-ETHYLCROTONALDEHYDE
3,6-Dichloropyridazine
4-Methylbenzyl acetate
3-(2-Bromoacetyl)benzonitrile , 95%
1,2,3,4-Tetrahydrocarbazole, 99%
Bis(trimethylsilyl)selenide
Thiopropionamide