OKT 3 Cell：小鼠杂交瘤复苏贴壁细胞系 货号：GD-C6420052
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞，T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞，可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞，并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Cyclohexyl p-toluenesulfonate, 97%
3-Mercaptopropionic Acid Isopropyl Ester
DESYL CHLORIDE
Diethylene Glycol Monovinyl Ether (stabilized with KOH), 96%
2-MethylbenzaMide
3,4-Methylenedioxyacetophenone
2,2,3,4,4,4-HEXAFLUORO-1-BUTANOL
Purine, 99%
5'-Fluoro-2'-methoxyacetophenone
Methyl Hydroxyethyl Cellulose (20-40mPa.s, 2% in Water at 20deg C)
4-(Chloromethyl)-1H-imidazole hydrochloride
DANSYL-L-PHENYLALANINE
Tetrahydrofurfuryl chloride
1-(4-METHOXYPHENYL)-1H-PYRROLE
Tosylmethyl isocyanide
5-Nitrothiophene-3-carboxylic acid
(4R,5R)-1,3-DiMethyl-4,5-diphenyl-2-[(S)-1-benzyl-2-hydroxyethyliMino]iMidazolidine
N-Cyclopropyl 2-broMobenzaMide
ThioacetaMide, 99+%, ACS reagent
o-PhenylenediaMine, Reagent
2-AMinophenol Hydrochloride
drofenine hydrochloride
2-Methylpyrimidine
3-iodoanisole
2,4-Dichlorobenzoyl peroxide
3-CHLOROBENZYLIDENEACETONE
Bismuth trichloride
Acid Red 27
Bis(2,2,6,6-tetraMethyl-3,5-heptanedionato)MagnesiuM(II) hydrate, 98+%
Methyl 2-Methylnicotinate, 97%
Stearyl Methacrylate
Methyl 3-thiophenecarboxylate
N-Methoxy 3-borono-4-fluorobenzaMide
ScandiuM(III) chloride hexahydrate
2-Methoxyisonicotinohydrazide
N,N'-Bis(2-aMinoethyl)-1,3-propanediaMine
2-(4-Fluoro-phenyl)-4-methyl-thiazole-5-carboxylic acid
Ethyl 3-[(6-methylpyrazin-2-yl)oxy]benzoate , 97%
N,N'-Diacetylethylenediamine
Ethyl 3-amino-4-cyano-5-(methylthio)-thiophene-2-carboxylate
1-NAPHTHALENEACETIC ACID
3-Mercapto-1,2-propanediol
Vagistat
Cupric acetate
Perchloric acid
N-(3-AMinopropyl)glycine Dihydrochloride
N-Benzyl-4-(benzylaMino)picolinaMide
3-Methylpyrazole, 99%
N-BOC-p-phenylenediaMine, 97%
4-NITROPHENYL-ALPHA-D-GLUCOPYRANOSIDE
4-Hexylaniline
Ethylene glycol
Fmoc-DL-serine
(1R,2R)-1,2-Cyclohexanedimethanol
2-Amino-5-bromobenzonitrile
2,4-Dibromophenol
TetraMethylguanidine
6,6-DIMETHYLBICYCLO(3.1.1)HEPT-2-ENE-2-ETHANOL
3-NORADAMANTANECARBOXYLIC ACID
Phenyl trifluoroacetate 98%
Diphenylamine Sulfate
4-Fluoro-2-methoxybenzyl alcohol
5-Methyl-1-phenylpyrazole-3-carboxylic acid
2-Deoxy-D-glucose
Amrinone
Homovanillic acid
3-Thiopheneboronic acid
(S)-(-)-alpha-Methylbenzyl isocyanate
1,3-Benzodioxole-5-carbothioaMide
4'-Hexyloxybenzylidene-4-cyanoaniline
Boc-3-aMinoMethyl-phenylacetic acid
3-Nitroanisole, 99%
TRILAURIN
N-(tert-Butoxycarbonyl)-4-chloro-L-phenylalanine
1,12-diaminododecane
Silicon dioxide
2-Nitrophenylacetic acid
Lionolblue GS
AT-2 ELISA Kit
γABRα2 ELISA Kit
Lipase ELISA Kit
Human histatin 5,HTN5 ELISA Kit
T3 ELISA Kit
COL1 ELISA Kit
GSTs ELISA Kit
Human very late appearing antigen 4,VLA4 ELISA Kit
Human androgen receptor,AR ELISA Kit
SIGLEC12 ELISA Kit
Czapek Dox Agar
Bean Agar Medium
Methyl indole-3-carboxylate, 99%
Mordant Blue 29
5-AMinopyridine-2-carboxylic acid, 98%
2-Methyl-2-Thiopseudourea HeMisulfate
drofenine hydrochloride
Sodium Molybdate Dihydrate
4-Methylmorpholine-N-oxide
Verapamil hydrochloride
TRIS(6,6,7,7,8,8,8-HEPTAFLUORO-2,2-DIMETHYL-3,5-OCTANEDIONATO)YTTERBIUM
Cbz-L-tert-Leucine
3,4,5-TribroMopyrazole
2-BroMoiMidazole, 95%
4-Fluoropiperidine hydrochloride, 95%
Lys-Lys-Lys
1-BroMo-3-chlorobenzene
2-Methoxythiophene
Nitroethane
L-(+)-Fructose , 97%
1-Cyclohexyl-1-pentanol, 98%
trans-4-Hydroxystilbene, 98%
(E)-(Hexen-1-yl)boronic acid 98%
4'-Fluoro-[1,1'-biphenyl]-4-carboxaldehyde
Nitrosobenzene
