NCI-H2452 Cell:人间皮瘤复苏贴壁细胞系 货号:GD-C6419700
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2'-Hydroxyacetophenone
IMidazole-2-carboxaldehyde
4,4'-Bis(chlorosulfonyl)diphenyl ether
1,3-DIBROMOACETONE
2-(BroMoMethyl)tetrahydro-2H-pyran
Heptyl forMate
2,3,4,5-Tetrafluorobenzoic acid ,98%
alpha-Tetralone, 85%
5-Carboxyfluorescein, 95%
H-Asn(Trt)-OH
ARACHIDIC ACID METHYL ESTER
Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
Hydrogen bromide
(4-FLUOROPHENYLTHIO)ACETONITRILE
Methyl 2,6-dichloroisonicotinate
2-Chloro-4-nitrophenol
2,2'-Ethylenedioxydiphenol
5-AcetaMidonicotinic acid
2,6-Difluorophenylacetone, 98%
DICHLONE
CalciuM 3-Methyl-2-oxovalerate Hydrate
1,2-Bis(2-pyridyl)ethylene
Casein
mercaptodimethur
3-Methyl benzyl chloride
Thiophene-2-ethylamine
POTASSIUM TETRACHLOROCUPURATE(II) DIHYDRATE
2-Chloro-4-Fluorobenzenesulfonyl Chloride
2-Hexanoylfuran, 99%
2-Iodobutane, 99%, stab. with copper
Maltose solution
4,6-DichloropyriMidine-5-carboxaldehyde
6-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE
N,N,N',N'',N''-PENTAKIS(2-HYDROXYPROPYL)DIETHYLENETRIAMINE
1,8-DIAZABICYCLO[5.4.0]UNDEC-7-ENE HYDROTRIBROMIDE
4-METHYL-2-PIPERAZIN-1-YL-QUINOLINE
6-Fluoro-4-hydroxy-2-(trifluoromethyl)quinoline
Fmoc-4-Chloro-D-phenylalanine
(S)-(-)-2-Isocyanato-4-(methylthio)butyric Acid Methyl Ester
(4-Bromophenoxy)-tert-butyldimethylsilane, 97%
2'-TRIFLUOROMETHYL-BIPHENYL-4-CARBALDEHYDE
Potassium hydrogen 2-oxoglutarate
3,5-DIBENZYLOXYBENZOIC ACID
XANTHOSINE DIHYDRATE
3-BroMopropionic acid
1,2,4-Benzenetriol
2-Methyleneglutaronitrile
Methyl 1-tert-butyl-1H-benzo[d]iMidazole-6-carboxylate
[3-Chloro-5-(trifluoroMethyl)-2-pyridyl]MethylaMine hydrochloride, 97%
Enalapril maleate
3-Dodecyl-2-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)thiophene
zirconyl chloride
6-Gingerol
3-Coumaranone
2,3,5-triacetylinosine
4-Bromophenylacetonitrile
3,9-DODECADIYNE
TriisopropylphosphoniuM tetrafluoroborate
[2-[2-(FMoc-aMino)ethoxy]ethoxy]acetic acid
DL-1-Amino-2-propanol
2-methyl-6-nitrobenzonitrile(SALTDATA: FREE)
4-(Trifluoromethyl)styrene, stabilized with 0.1% 4-tert-butylcatechol, 98+%
5-Bromo-2'-deoxycytidine ,98%
1-METHYL-1H-IMIDAZOLE-5-CARBOXALDEHYDE
3-Pyridinecarboxaldehyde
1-Octen-3-yl acetate
1,5-DIAMINO-2-METHYLPENTANE
L-Phenylalanine
2,3,4,5,6-Pentafluorothiophenol
Methyl 4,4,4-Trifluoroacetoacetate
2,4-DichloropyriMidine, 97%
2,3-DibroMopyridine, 97+%
Sodium 4-phenylbutyrate
PotassiuM (2-ForMylphenyl)trifluoroborate
sodium dihydrogen phosphate anhydrous
methyl 3-[3-(2H-benzotriazol-2-yl)-5-tert-butyl-4-hydroxyphe...
4-Methyl-5-imidazolemethanol hydrochloride
2-[(4-methylphenyl)sulfonyl]-1,2,3,4-tetrahydroisoquinoline
Tie2 ELISA Kit
GRIA4 ELISA Kit
IGFBP1 ELISA Kit
Human LMWH ELISA Kit
A-FABP ELISA Kit
Rat CD8 (Cluster of Differentiation 8) ELISA Kit
HB ELISA Kit
Human paraoxonase,PON ELISA Kit
Human IGF-1 ELISA Kit
PⅢNP ELISA Kit
Glucose Asparagine Agar(Waksman, 1961)
King’s B Medium
LITHIUM OXALATE
trans-1-Octenylboronic acid, 98%
4-Ethynylbenzonitrile 97
2-AMinobenzothiazole-6-carboxylic acid, 95%
1,2-Bis(2-pyridyl)ethylene
(bromomethyl)cyclobutane
5-(Bicycloheptenyl)trichlorosilane
D-Threonine
1-Ethylpyridinium chloride
BIS(2-BUTOXYETHYL) ADIPATE
Thiophene-2-Boronic Acid
3-Chloro-2-fluoro-5-(trifluoroMethyl)benzoic acid, 97%
4-Iodo-M-xylene, 98%
Oxazole
2-Methoxy-4-Methylbenzoic acid
STRONTIUM ZIRCONATE
3-Fluoro-4-(trifluoroMethyl)aniline, 97%
BroMo-tris-pyrrolidino-phosphoniuM hexafluorophosphate
Atenolol
Poly(acrylamide), granular, non-ionic, approx. M.W. 5 to 6.000.000
2-Bromofuran-4-carboxylic acid
Ethyl cyclohexylacetate, 98+%
N-(1-Adamantyl)urea, 98%