LOVE1 Cell：人结直肠癌复苏贴壁细胞系

LOVE1 Cell：人结直肠癌复苏贴壁细胞系 货号：GD-C6419670
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
7-(2,3-Dihydroxypropyl)theophylline
MagnesiuM sulfate anhydrous Standard
2-(Benzyloxy)-1-chloro-3-fluorobenzene
BIS(4-METHOXYPHENYL)CHLOROPHOSPHINE
4-BUTYL-4'-HYDROXYCHALCONE
4-BroMo-3-chlorophenylboronic acid
7-ethyl-1H-indole-2,3-dione(SALTDATA: FREE)
5-Carboxyphthalide 97%
4-Bromo-3-methoxyaniline
5-tert-Butyl-m-xylene
(-)-ANABASINE
BENZO-12-CROWN-4
1,3-Diphenylisobenzofuran
CERIUM(III) CHLORIDE
6-Morpholinonicotinic acid
n-Butylbenzene
Ethyl Methyl Sulfone
Ac-Arg-OH 2H2O
4-Pyridazinecarboxylic acid, 95%
CLOMAZONE
(-)-4,5-Bis[hydroxy(diphenyl)Methyl]-2,2-diMethyl-1,3-dioxolane
aloin
Silver iodate
ethyl-1,1-D2 alcohol
2-Bromo-4'-methylacetophenone
Chelidamic acid
1,4-Dioxaspiro[4.5]decan-8-one
2,3-DiMethoxynaphthalene, 97%
Tetraphenylphosphine iMide, 97%
3-BroMo-2-Methylbenzotrifluoride, 97%
Hexyl salicylate
Methyl cinnaMate
Voglibose
N,N'-DICYCLOHEXYLUREA
YTTERBIUM(III) CHLORIDE HYDRATE
M-CRESYL ACETATE
1-Bromo-4-n-heptyl benzene
4-[2-(4-Nitrophenoxy)ethyl]morpholine 98%
2-Nonenoic Acid
Osmium, 99.9%
ANTIMONY (III) SELENIDE
2-METHOXY-4-(TRIFLUOROMETHYL)PYRIDINE-5-CARBOXAMIDE
3-CHLORO-2-FLUORO-5-(TRIFLUOROMETHYL)BENZALDEHYDE
Prostaglandin A1
N-Acetyl-L-leucine
Meso-Butane-1,2,3,4-tetracarboxylic Acid
3-BroMo-2,6-difluorophenylboronic acid
5-Methoxy-2-MercaptobenziMidazole, 99+%
1,2,3-Trifluoro-4-nitrobenzene, 97%
Quinaldine
DL-HoMocysteinethiolactone Hydrochloride
2,3-Dibromosuccinic acid
(R)-N-FMOC-3-Fluorophenylalanine
Estr-9-en-3-one, 11-[4-(dimethylamino)phenyl]-5,17-dihydroxy-17-(1-propynyl)-cyclic 1,2-ethanediyl acetal,(5a,11b,17b)-
Anagrelide Hydrochloride
DIBENZOTHIOPHENE SULFONE
Boc-α-(4-broMobenzyl)-DL-Pro-OH
4-BroMopyrazole
PONCEAU SX
1,3,5-Tribromobenzene ,98%
4,4'-Dihydroxybenzophenone ,98%
2-Thien-2-ylbenzaldehyde, 97%
4-chloro-2-methylbenzenesulfonyl chloride(SALTDATA: FREE)
3,4-DIAMINOTHIOPHENE DIHYDROCHLORIDE
4-BENZYLOXYPHENYL ISOCYANATE
5-ACETYL-2,3-DIHYDROBENZO(B)FURAN
Furfuryl mercaptan
2,4,6-TriMethylbenzylaMine
2,2'-Bis(diphenylphosphino)biphenyl
Ethyl Hydrogen Maleate
3-Chloro-N-Methyl-5-(trifluoroMethyl)pyridin-2-aMine
Ethyl Myristate, 98+%
Magnesium bromide hexahydrate
2-Methylheptane
alpha-Ionone
triethylene glycol monohexyl ether
Iodine
1-bromoheptadecane
UP ELISA Kit
Mouse BAD (Bcl-2 Associated Death Promoter) ELISA Kit
Human IGFALS ELISA Kit
Human LRP-5 ELISA Kit
STRAIL-R1 ELISA Kit
Rat Leukotriene B4,LT-B4 ELISA Kit
EPAS1 ELISA Kit
CHRM2 ELISA Kit
INS ELISA Kit
Mouse Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit
Swine plague vaccine medium
Gause’s Synthetic Agar Medium,Modified
Ethyl 2-aMino-5-nitrothiophene-3-carboxylate, 95%
trans-3-FluorocinnaMic acid, 98%
4-Ethynylbenzeneboronic acid pinacol ester, 95%
2-AMinoacetophenone
aloin
2,4-dihydroxy-6-methyl-5-*nitropyrimidine
4-methylthiophene-2-carboxylic acid
n-Docosane

Methylparaben
CARAZOLOL HCL
2-AMino-3-pyridinecarboxaldehyde
Cyclohexanethiol, 99%
1-BroMopinacolone, 97+%
CaloriMetric
Dicyclohexyl phthalate
TRIMETHYLSILYL BROMOACETATE
4-tert-ButylbenzylaMine
1-(2-BROMO-PHENYL)-PYRROLE-2,5-DIONE
Pentaerythritol Tetra(3-Mercaptopropionate) , 85%
Ammonium fluoride, 0.5M solution in methanol, AcroSeal
1-(4-Fluorophenyl)-2-pyrrolidinone, 99+%
Vinyltris(methylethylketoximine)silane
Isopropyl (2E,4E,7S)-11-methoxy-3,7,11-trimethyldodeca-2,4-dienoate