COLO 741 Cell：人直肠癌复苏贴壁细胞系

COLO 741 Cell：人直肠癌复苏贴壁细胞系 货号：GD-C6420126
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞，T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞，可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞，并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
7-AMinocephalosporanic acid, 98% (dry wt.), May cont. up to 2% water
3,3′-Dichlorobenzidine dihydrochloride
N-(4-IODOPHENYL)MALEAMIC ACID
1-Boc-3-fluoro-4-pyrrolidinone
Paliperidone
4-Phenylbutanol
DIHYDROCHLORIDE HYDRATE 98
tert-Butylamine
DL-2,6-Difluorophenylalanine
Heptadecanoic acid, 98%
N-(4-hydroxyphenyl)-N-methylmethanesulfonamide(SALTDATA: FREE)
2-Pyrazinecarboxylic acid
3-Nitrophenylboronic acid
Vardenafil dihydrochloride
Molecular sieves 4A
Propylene carbonate
Diethyl 3-Hydroxyglutarate
3,4-DibroMopyridine
trans-2-Octene, 98%
Phosphoric Acid, Meta, Chip, Reagent, ACS
Penta-O-acetyl-beta-D-glucopyranose
4-Bromoisopropylbenzene
1-tert-butylpiperidin-4-ol
Lithium methoxide
2-Amino-6-purinethiol
RUBIDIUM MOLYBDENUM OXIDE
M-AMinobenzyl Alcohol
[Rh COD (R)-AnPhanephos]BF4, Rh 10.4%
Benzyl Methacrylate, 98%, stab. with ca 50-100ppM 4-Methoxyphenol
4,5-DiMethoxy-2-nitrophenylacetic acid, 97%
4-(TriMethylsilyl)Morpholine
4-BroMo-3-chloroanisole
3-BroMo-2-Methyl-6-Morpholinopyridine
PyriMidinetetraMine sulfate
3-METHOXYBIPHENYL
COUMARIN 7
4-Bromo-N-butylbenzamide
H-D-Arg(Pbf)-OH
2,2-Dimethyl-2,3-dihydro-1-benzofuran-7-carboxylic acid , 97%
(6S)-1,4-Diazabicyclo[4.3.0]nonane
trans-3-Indoleacrylic acid
3,5-Dimethoxybenzyl chloride
Benzoyl chloride
4,7-DIBROMO-2,1,3-BENZOTHIADIAZOLE
4-BroMo-2-(trifluoroMethoxy)aniline
1-BroMo-2,3,5-trichlorobenzene
2-Chloro-6-fluoro-5-Methylphenylboronic acid
Betaine, 98%, for analysis, anhydrous
1-(3-Isocyanatophenyl)-1H-pyrrole, 97%
Benzophenone DiMethylketal
Carbonylchlorobis(triphenylphosphine)iridiuM(I)
3-chloro-2,2,3,3-tetrafluoropropanoic acid
Boronic acid, 2-dibenzofuranyl-
4,6-Dichloro-1H-indole-2-carboxylic acid
morantel citrate
1-[3,5-BIS(TRIFLUOROMETHYL)PHENYL]PYRROLE
(3r,4r)-1-benzyl-4-hydroxy-3-pyrrolidineMethanol
4-N-BUTYL-2-METHYLPHENYL ISOTHIOCYANATE
Ethyl 2-hydroxy-5-nitronicotinate
4-(4-Methoxyphenyl)pyrimidine-2-thiol , 95%
Erucic Acid
4-Bromo-N-(3-chloropropyl)benzenesulphonamide 98%
3-Decylthiophene
3-FLUORO-4-(4,4,5,5-TETRAMETHYL-[1,3,2]DIOXABOROLAN-2-YL)PYRIDINE
2,6-DIMETHYLNAPHTHALENE
4-Hydroxy-2-(trifluoromethyl)pyrimidine
Genistin
2,2-DiMethylsuccinic Anhydride
1,4-Anhydro-D-xylitol
2-(N-BenzylsulfaMoyl)-5-Methylphenylboronic acid
4-Methylcyclohexanone, 98%
Cyclohexylhydrazine hydrochloride, 98%
TETRAPHENYLARSONIUM CHLORIDE
p-Xylenol Blue
1,1,1,2,2,3,3,4,4-nonafluoro-4-iodobutane
2-Thioanisole Phenylborate
3-(Acetylthio)-2-methylpropanoic acid
N-(4-bromophenyl)cinnamamide
GP-Ⅱ ELISA Kit
Mouse Interferon β,IFN-β/IFNB ELISA Kit
Human ITGα9 ELISA Kit
RVHIAb ELISA Kit
Chicken soluble E-selectin,sE-selectin ELISA Kit
Rat GAS (N-Acetylgalactosamine 6-Sulfatase) ELISA Kit
Chicken L-Phenylalanine ammonla-lyase,PAL ELISA Kit
Human CCL21 ELISA Kit
Human angiotension Ⅱ，ANG-Ⅱ ELISA Kit
CYP7A1 ELISA Kit
Yeast Synthetic Drop-out Fluid Medium without Urac
Malt Extract Broth
Phenylethyl Isovalerate
PotassiuM perruthenate(VII), 97%
5-AMino-1H-Tetrazole
TSTU
4-Bromoisopropylbenzene
Cyanomethyl benzenesulfonate
Aluminum chloride hexahydrate
2,5-Dinitrophenol

4-NITROBENZENESULFONIC ACID
Oxiranemethanol acetate
PotassiuM Chloride
2,6,8-TriMethyl-4-nonanone, 98%
3-(TrifluoroMethyl)benzyl broMide, 98%
3,5-Difluoro-4-hydroxybenzaldehyde
Arachidic acid
Diethyl (2-cyanoethyl)phosphonate
3,4-DIHYDROXYBENZALDOXIME
2,5-BIS(4-NITROPHENYL)-1,3,4-OXADIAZOLE
DIPHENYLTETRAMETHYLDISILANE
N-(1-Naphthyl)phthalamic Acid
Bromocresol Green (0.04% in ca. 50% Ethanol) [for Titration]
4-(4-Chloro-3-methylphenoxy)-3-nitro-benzotrifluoride
(S)-2-Aminomethyl-1-N-Cbz-pyrrolidine