SK-BR-3[SKBR3]细胞：人乳腺癌复苏细胞系更多折扣

SK-BR-3[SKBR3]细胞：人乳腺癌复苏细胞系更多折扣 货号：GD-C5920370
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-BroMo-2-(1H-pyrazol-3-yl)phenol, 98%
4,4′-Oxybis(benzoic acid)
N-(4-CARBETHOXYPHENYL)MALEAMIC ACID
3-CHLORO-2-METHYLPHENYL ISOCYANATE
POE (20) ISOHEXADECYL ETHER
ISOVALERIC ANHYDRIDE
POTASSIUM HEXACHLORORUTHENATE(IV)
3-(Dimethylamino)butan-1-ol
3-(Pyrrolidin-2-yl)pyridine
2-{4-[(4-Methylphenyl)sulfonyl]piperazino}-1-ethanol
2,3-Dibromo-6,7-dicyanonaphthalene
ACETALDEHYDE DI-N-HEXYL ACETAL
Diisopropyl chlorophosphate
GOLD(I) CYANIDE
2-BroMobenzyl Alcohol
3-DiethylaMinophenol
Desloratadine
2-AMino-5-Methylnicotinaldehyde
Vinyl broMide, 1M solution in THF, AcroSeal
SodiuM Stearate, Powder, NF
Geraniol
Pivalaldehyde oxime
L-thyroxine sodium salt
3-tert-butyltoluene
1-(dibromomethyl)-3-fluorobenzene
3-ISOCYANATOBENZOYL CHLORIDE
2-NaphthylaMine-6-sulfonMethylaMide

2-BroMothiobenzaMide, 97%
4-Fluoro-2-Methylphenylacetonitrile, 98%

Acid Blue 119
P-QUATERPHENYL
PentabroMophenol
D-Tryptophanol
CyclohexylaMine hydrobroMide
Ethyl 5-methyl-1-phenylpyrazole-3-carboxylate
2-Nitro-1,3-indandione Dihydrate
2-Chloro-5-nitroanisole
Diallyl Pyrocarbonate
TRANS-1,2-DIBROMOCYCLOHEXANE
2-BROMO-6-FLUOROBENZONITRILE
2,3-DIBROMOPROPIONAMIDE
4H-3,1-Benzoxazine-2,4(1H)-dione
2-HydroxybenzylaMine
2-Chloropropiophenone
1-(Pyridin-3-ylMethyl)pyrazole-4-boronic acid
Indene, 90%, tech., stabilized
DiphenyliodoniuM trifluoroMethanesulfonate, 99%
5-AMinoorotic Acid
2,4-Dichlorobenzotrichloride
Allylmalonic acid
Diphenyl carbonate
6-(1-Bromoethyl)-4-chloro-5-fluoropyrimidine
Donepezil
Sodium cholate
N-(P-TOLUENESULFONYL)-3-PYRROLINE
BOC-LYS(Z)-OH DCHA
5-(BenzyloxycarbonylaMino)pyridine-3-boronic acid, pinacol ester
1,2-Dibromo-3-chloropropane
3-Chloro-1,2-benzisothiazole
1-Cbz-3-Aminopyrrolidine hydrochloride
NBQX
3-Methylthiopropanol
Pentasodium DTPA
Bis(1,2,2,6,6-pentamethyl-4-piperidyl) [[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]butylmalonate
1,3-PhenylenediaMine;M-PhenylenediaMine;1,3-DiaMinobenzene;1,3-BenzenediaMine
N,N′-DisucciniMidyl carbonate
1-BroMo-2,4-diMethoxybenzene
Methyl n-Octyl Sulfide
N-(2-Chloro-3-hydroxypyridin-4-yl)pivalaMide
Tetrahydro-beta-carboline, 98%
3-HYDROXY-2-NAPHTHOIC ACID HYDRAZIDE
7-AMinoheptanoic Acid
1-carboxaldehyde
1,8-diiodooctane
4-amino-2-methoxybenzonitrile
1-Nonyne
FETU-A ELISA Kit
Smad3 ELISA Kit
P ELISA Kit
Human E1/ubiquitin-activating enzyme,E1/UBAE ELISA Kit
IL12A ELISA Kit
α-GST ELISA Kit
CKM ELISA Kit
SREBP ELISA Kit
Human HO ELISA Kit
Guinea pig INS (Insulin) ELISA Kit
Eugon LT 100 Broth Base
Eugon Agar
AcetazolaMide, 99%
Silver(I) fluoride, 98%
5,6-Diethyl-3-(2-pyridyl)-1,2,4-triazine, tech. 90%
2-Fluoro-4-Nitroanisole
Pivalaldehyde oxime
(R)-(-)-2-Aminobutane
Molybdenum(V) chloride
beta-thujaplicin

2-Ethoxynaphthalene-1-carbonyl chloride
6,7-DiMethoxy-3,4-Dihydroisoquinoline
Chlorophyllin, coppered trisodiuM salt
4-n-Propoxy-3-Methylbenzeneboronic acid, 97%
3-Chloro-4-iodopyridine, 97%
2.4-Dinitrophenol Standard
1,2-DIHYDRONAPHTHALENE
4-BroMo-2-furaldehyde
BisMuth neodecanoate
(3,4-DiaMinophenyl)phenylMethanone
P-TOLUENESULFONIC ACID 3-NITROPHENYL ESTER
2-Hexadecyl-malonic acid
2,8-Dimethylimidazo[1,2-a]pyridine-3-carboxylicacid
3,3-dioctadecyloxacarbocyanine perchlorate (DiO;dIoc18(3))
alpha-Methyl-3,5-bis(trifluoromethyl)benzyl alcohol 99+%