HS-578T细胞:人乳腺癌复苏细胞系更多折扣 货号:GD-C5920101
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-BroMo-2-Methoxypyridine-4-boronic acid, 98%
Bis(2-butoxyethyl) phthalate
6-Nitroveratraldehyde
ETHYLENE GLYCOL ETHYL METHYL ETHER
Isoflurane
3-Benzyloxy-5-fluorophenylboronic acid
CARBAMIC ACID ISOPROPYL ESTER
5-Chloropyridazin-3(2H)-one , 97%
2-(Phenylsulfonyl)ethanol
N'-Nitrosonornicotine-N-b-D-glucuronide ,98%
4,5-Dimethoxy-2-(methoxycarbonyl)phenylboronic acid
Boc-O-benzyl-L-tyrosine
Anilofos
(R)-(+)-4-CHLOROMETHYL-2,2-DIMETHYL-1,3-DIOXOLANE
SAMARIUM
Thieno[2,3-b]thiophene-2-carboxylic acid
4,4'-Dichloro-trans-stilbene
2,4-DibroMo-1-Methyl-1H-iMidazole
Tolylene 2,6-diisocyanate, 97%
PHENANTHRENE
2-BroMo-6-Methoxypyridine
Glutamine
4-Benzenesulfonylbenzoic acid
2,5-Diphenyl-p-benzoquinone
1,3-Dimethoxy benzene
5-HEXENOIC ACID
Methanesulfonic anhydride
6-ChloronicotinaMide
SodiuM butyrate, 98+%
1-Phenyl-1H-pyrazole-4-carboxaldehyde, 98%
1-Chlorododecane
4,6-DihydroxypyriMidine
2-Chloro-1,3-thiazole-5-carbonitrile
RhodiuM(II) triMethylacetate, diMer
4,6-Dichloro-5-pyriMidinecarbaldehyde
Etidronic acid Monohydrate
Hexyl alcohol, pract., 96%
4-Benzylamino-1-butanol
1H-Pyrrolo[2,3-b]pyridine-5-carboxylic acid methyl ester
Benzyl 2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside
BENZO(K)FLUORANTHENE
Urethane
2-(N-Morpholino)ethanesulfonic acid potassium salt
IODINE MONOBROMIDE
1-(4-BroMophenyl)pyrrolidine
Dicyclohexyl phthalate
2'-Nitroacetanilide
Tetrahydrothiophene-3-oxo-1,1-dioxide
2-Naphthyl sulfate, potassiuM salt, 99%
1,2,3-HEXANETRIOL
1,3-PhenylenediaMine Sulfate
o-Bromobenzyl cyanide
3-[(1R,3R)-3-{[(Dimethylcarbamoyl)amino]methyl}-3,5,5-trimethylcyclohexyl]-1,1-dimethylurea
Diammineplatinum(II) nitrite
acid red 114
4-Bromobenzaldehyde
AMMoniuM fluoborate
3-Chloroisoquinoline
N-BroMoisocyanuric acid MonosodiuM salt
2-ForMylbenzeneboronic acid
3-Chlorothiophene-2-carboxamide ,99%
Deoxycholic acid, 98.5-101%
Uridine 5′-diphospho-N-acetylglucosamine sodium salt ,98%
Boc-L-citrulline
(R)-Tomoxetine
Bis(2,4-dimethylpentane-2,4-glycolato)diboron
1-(2-CHLORO-4-NITROPHENYL)-
4-AMinopyriMidine
1,3-Dichloro-1,1,3,3-tetraisopropyldisiloxane
Dithieno[3,2-b:2',3'-d]thiophene
1H,1H-UndecafluorohexylaMine
Tris(4-trifluoroMethylphenyl)phosphine, 99%
Silver Lactate
2-Chloro-6-fluorotoluene
4-Chlorobenzyl bromide
2-Furylglyoxylonitrile
1,4-Butanediol diacrylate
2-Nitro-4-Fluoro Benzoic Acid
ANP ELISA Kit
βEP ELISA Kit
Human regulated on activation in normal T-cell expressed and secreted,RANTES ELISA Kit
Human CLDN8 ELISA Kit
Chicken Immune RNA,Irna ELISA Kit
ND1 ELISA Kit
5-NT ELISA Kit
ER ELISA Kit
Human ANG ELISA Kit
CTLA4 ELISA Kit
Potato dextrose agar
Staphylococcus Enrichment Broth Base
IsonicotinaMidoxiMe, 97%
MagnesiuM aluMinuM zirconiuM oxide, 99% (Metals basis excluding Ca) Ca 0.1-1%
5-AMino-2-chloropyridine, 98%
2-Cyclopenten-1-one, 98%
Glutamine
Dysprosium nitrate
Sodium perchlorate
2-Oxa-8-azaspiro[4.5]decane
11H-PERFLUOROUNDECANOYL CHLORIDE
PHENYLTHIOHYDANTOIN-THREONINE
N-Benzyl-3-Pyrrolidinol
1,2-Dichloro-4-iodobenzene, 98%
(2R,5R)-1-(2-[(2R,5R)-2,5-DiMethylphospholan-1-yl]phenyl)-2,5-diMethylphospholane 1-oxide, 97+%
4-(TrifluoroMethylthio)benzyl broMide
Diphosphoryl chloride
2-FluorobenzylaMine
(2,5-DiMethoxyphenyl)acetic acid
N-Ethyl-N-(3-sulfopropyl)-M-anisidine sodiuM salt
3-tert-Butyl-1-Methyl-2-pyrazolin-5-one, 98%
2-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)aniline, 97%
1,5-Dimethyl-2-piperidone
Zirconium(IV) oxide, 98.5%
Boc-2-Abz-OH