BGC823细胞:人胃腺癌复苏细胞系更多折扣 货号:GD-C5920061
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-AMino-2-fluoro-4-Methylpyridine, 95%
Methyl 4-aMino-3-Methylbenzoate
ZINC ARSENIDE
N-Methyl 4-chloropicolinaMide
Cyclopropyl 5-broMo-2-chlorobenzaMide
Ethyl (4-iodobenzoyl)acetate
2,5-Diethoxybenzaldehyde
2,3-Dihydro-5-(hydroxymethyl)benzo[b]furan
4-Isopropoxy-2-butanone
7-Methylimidazo[1,2-a]pyridine-6-boronic acid
2-Chloro-4-nitrophenyl-β-D-maltotrioside ,97%
2-CHLORO-6-FLUOROBENZYL BROMIDE
Bismuth subcarbonate
5-Phenyltetrazole
1-Aminocyclopropane-1-carboxylic acid hydrochloride
Cyclopentylacetic acid
TributyldodecylphosphoniuM BroMide
N-Cyclopropyl 3-broMo-5-trifluoroMethylbenzenesulfonaMide
TetrabutylaMMoniuM fluoride, 1M solution in THF, containing ca. 5% water
o-Toluic Acid
4-AMino-3,5-dichloropyridine
1-Nitro-2-naphthol
Alpha-Bromo-Gamma-butyrolactone
2-Amino-4,6-dimethylpyrimidine
trichlormethiazide
Calcium stearate
FUMARONITRILE
2,6-Dichloro-5-fluoro-3-pyridinecarbonitrile
3-Methoxybenzonitrile, 98%
Zinc trifluoroMethanesulfonate, 98%
4-BroMo-2,6-difluorobenzonitrile
Indole-6-carboxaldehyde
1-DiethylaMino-2-propyne
SAMARIUM(III) TRIFLUOROMETHANESULFONATE
L-GlutaMic acid alpha-benzyl ester
Ethyl cis-(β-cyano)acrylate
Methyl isocyanoacetate, tech., 95%
LY-294002
4-Acetoxybiphenyl
3-Bromo-1-fluoronaphthalene
4,4'-Diamino-2,2'-stilbenedisulfonic acid
CYTIDINE 5'-DIPHOSPHOCHOLINE
2-Benzyloxyphenylboronic acid
Abscisic acid
4-Iodobiphenyl
2,6-Di-O-Methyl-β-cyclodextrin
alpha-[2-(MethylaMino)ethyl]benzyl Alcohol
4-Fluoro-3-Methoxyaniline, HCl
SodiuM cyclopentadienide
Phenethyl caffeate
4-Chloro-2-iodoaniline
Triallyltrimesate
2-Amino-4,6-dimethoxypyrimidine
Ethyl 3-methylthiopropionate
tert-butoxy-bis(dimethylamino)methane
Phenazine
7-Methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene , 95%
ETHYL 3-METHOXYPHENYLACETATE
3-IodobenzylaMine hydrochloride
2-Aminopyrimidine
2-Bromo-1-methyl-1H-imidazole-4,5-dicarboxylic acid , 97%
3'-Chloro-2',6'-difluoroacetophenone
2,6-Difluorobenzenesulfonamide
1-Boc-5-Cyanoindole-2-boronic acid
Nonylphenol disulfide oligomer
BCP
Acethydrazide
3-(1H-IMidazol-1-yl)propanoic acid
1,3-Bis(2,6-diisopropylphenyl)iMidazolidin-2-ylidene
4-(1-Ethoxycarbonylcyclopropyl)phenylboronic acid, pinacol ester
Dess-Martin periodinane, 15 wt.% solution in dichloroMethane, AcroSeal
2-Chloro-4-fluorophenol, 97%
N-Carbobenzoxy-L-alaninol
(S)-2-Methylbutyl p-Toluenesulfonate
3,5-Dimethyl-1-phenyl-1H-pyrazole
azetidin-3-ylmethanol hydrochloride
7-[3-(Chlorodimethylsilyl)Propoxy-4-Methylcoumarin
Piperidin-4-ylidene-acetic acid hydrochloride
TA ELISA Kit
ACTg1 ELISA Kit
Human lipolysaccharide binding protein,LBP ELISA Kit
Human SPARC (Secreted Protein Acidic And Rich In Cysteine) ELISA Kit
Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit
Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit
RF ELISA Kit
Human PF ELISA Kit
Human ASD ELISA Kit
TUBa3C ELISA Kit
Glucose Peptone Medium
Starch Ammonium Sulphate Agar(Krassilnikov, 1950)
Indole-3-Methyl Acetate
Lead chromate
5-AMino-4-broMo-1-Methyl-1H-pyrazole, 98%
2-N-BUTOXYETHYL METHACRYLATE
1-Nitro-2-naphthol
Decyltrimethylammoniumchloride
4,4'-sulphonylbis[2-aminophenol]
Cephalothin
(+)-CIS-2-BENZYLAMINOCYCLOHEXANEMETHANOL
4,6-DIMETHYLPYRIMIDINE
4-Vinyl-1,3-Dioxolan-2-One
1,3-DibroMotetrafluorobenzene, 98+%
DiMethylzinc, 96%
3-Nitrophenol
Pentafluorophenol
OCTAFLUOROTOLUENE
TERT-BUTYL N-[3-(AMINOMETHYL)BENZYL]CARBAMATE
(2-[(MethylaMino)sulfonyl]phenyl)boronic acid
MethoxyphenaMine hydrochloride
Direct Scarlet B
4-(tert-Butyldimethylsiloxy)phenyl boronic acid
1,5-Dimethylnaphthalene
4-Fluoro-2-methylbenzenesulphonyl chloride