A-204[A204]细胞:人横纹肌肉瘤复苏细胞系更多折扣 货号:GD-C5920048
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 4-nitrocinnaMate, 99%
LithiuM trifluoroMethanesulfonate
Ethyl 4-MethoxycinnaMate
Isobutyl Salicylate , 99%
4,5,6,7-Tetrachloro-1,3-isobenzofurandione
5-AMinoindole
3-Ethylphenylboronic acid
N,N-Dimethyl-3-nitroaniline
Butyl Formate
Boc-D-Lys(Fmoc)-OH
5-Chloro-4-hydroxy-2(1H)-pyridinone
BIS(PHENYLSULFONYL)METHANE
3-BROMO-N,N-DIMETHYLANILINE
TETRAETHYL ETHYLENETETRACARBOXYLATE
2,8-Bis(trifluoromethyl)-4-quinolinol
CystaMine dihydrochloride
Didodecyl Sulfoxide
N-Cyclopentyl 2-broMo-5-fluorobenzaMide
TheobroMine, 99%
OMeprazole MagnesiuM, EP
Tris(triMethylsilyloxy)ethylene
2,4,5-7-Tetrabromofluorescein disodium salt
Fenspiride
3'-Bromophenyl acetylene
N-(trichloromethylthio)phthalimide
HEXACHLORODISILOXANE
5-CHLORO-2-METHYLINDOLE
2,6-Dichloroquinone-4-chloroiMide
Tetra-n-butylaMMoniuM hydroxide, 1.0M in Methanol
4-Methoxystyrene, 98%, stab. with 0.1% 4-tert-butylcatechol
1,3,5-TribroMo-2,4,6-TriMethyl-Benzene
1-Boc-3-hydroxypiperidine
1,4-Diiodobenzene
SCOPOLAMINE N-OXIDE HYDROBROMIDE
(DHQ)2PHAL
N-ETHOXYCARBONYL-5-NITRO-O-TOLUIDINE
1,1,2,2-Tetrachloroethane-d2, 99 atom % D, for NMR
1-(2-Aminoethyl)pyrrolidine
Acyl-coenzyme A Synthetase [>=8 units/mg]
Allyl(tert-butyl)dimethylsilane
PHENYL NICOTINATE
LINDANE
2-HEXANOYLFURAN
POLY(BISPHENOL A CARBONATE)
Pyrazine
Zinc acetylacetonate hydrate
5-(tert-ButyldiMethylsilyloxy)-1H-indole
3,4-Dihydro-2H-pyran, 99%
3-(Diphenylphosphino)propylaMine, 97%
S-[2-(4-PYRIDYL)ETHYL]-L-CYSTEINE
4-BroMopyrene
3-Methylindole
3-AMINO-3-(2-FLUORO-PHENYL)-PROPIONIC ACID
3-Pyridin-3-ylaniline
4-Chloropiperidine hydrochloride
1,2,5-TRIMETHYLPYRROLE
(1S,3S)-trans-4-Carene
Butyl 10-undecenoate
ACETOACETIC ACID N-BUTYL ESTER
Perrhenic(VII)acid, 76.5% solution in water, (trace metal basis), 99.99%
3,5-Bis(tert-butyldiphenylsilyloxy)benzyl Alcohol
1-(Fur-2-yl)-2-nitroethene 98%
Benzo[b]selenophene, 97%
1-Bromo-2-methoxyethane
3-Fluoro-2-methylbenzaldehyde
2,4'-DINITRODIPHENYLAMINE
DIPROTIN A
Methyl 3-Methoxyacrylate
1,3-AdaMantanediol
tert-butyl N-(pyrrolidin-2-ylMethyl)carbaMate
4,6-DiMethyl-2-pyrone, 98%
2-AMinopyridine-3-carbonitrile, 97%
Pyrazole
SulfaMethizole
Myrcene
3,4-Dihydro-2H-benzo[1,4]oxazine-7-carboxylic acid methyl ester
Fatty acids, C16-18 and C18-unsatd., Me esters
7-Methoxy-4-chromanone
ANG-Ⅱ ELISA Kit
IFN-γ ELISA Kit
Human LIPK ELISA Kit
Human CAMK 2 ELISA Kit
Chicken glial fibrillary acidic protein,GFAP ELISA Kit
Rat COL9α1 (Collagen Type IX Alpha 1) ELISA Kit
FSH ELISA Kit
Human PDCD4 ELISA Kit
ADG ELISA Kit
DUTP ELISA Kit
Wort Broth Base
ISP Medium 7( Tyrosine Agar Medium Base)
Indole-3-acetonitrile, 98%
Phloretin, 98%
5-CarbaMoylpyridine-3-boronic acid pinacol ester, 96%
2-METHYL-2-THIOPSEUDOUREA SULFATE
2,4,5-7-Tetrabromofluorescein disodium salt
Sodium thiosulfate pentahydrate
L-Phenylalanine methyl ester hydrochloride
aziridine
2-Nitro-3-pyridinamine
Amphotericin B
2-AMinothiazole-5-carboxaldehyde, 95%
Trichloroacrylic acid, 98%
BenzylMagnesiuM chloride, 1M in MeTHF
1-BroMo-3-fluoro-5-nitrobenzene
1,4-HEXADIENE
3-HydroxytyraMine hydrochloride
2-(3-Fluorobenzyloxy)phenylboronic acid
ISOPROPENYLOXYTRIMETHYLSILANE
4-(2-MethoxyethylcarbaMoyl)phenylboronic acid
5-Bromo-1H-benzimidazole, 97%
3-Amino-4-chlorobenzotrifluoride ,97%
3,3-Dimethoxyhexane
2-Fluorocyclohexanol