HCT-8细胞：人回盲肠癌复苏细胞系更多折扣

HCT-8细胞：人回盲肠癌复苏细胞系更多折扣 货号：GD-C5919257

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4'-BroMoheptanophenone, 97%
Salicylanilide
ETHYL HYDROGEN GLUTARATE
6-Chloro-2-fluoro-3-Methylbenzonitrile, 97%
4-Methoxy-2,3,6-triMethylbenzenesulfonyl chloride, tech. 90%
2',4'-DICHLOROPROPIOPHENONE
1-MethyliMidazole
Dicarbonylacetylacetonato iridium(I), 98%
2-(tert-Butoxycarbonylthio)-4,6-dimethylpyrimidine [Boc Agent for Peptides Synthesis]
1-Phenyl-propylamine
3-Fluorobenzyl mercaptan
2-Naphthoxyacetic acid
5-CHLORO-M-PHENYLENEDIAMINE
METHYL 2-CHLOROISONICOTINATE
4-[4-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)phenyl]Morpholine
3,4-DichlorophenethylaMine
4-BroMo-2,3-difluorophenol
Methyl 3-broMoiMidazo[1,2-a]pyridine-6-carboxylate
ZirconiuM(IV) oxide, 98.5%
Sudan II
Undecanoic Acid
o-Tolylacetic acid
D-Histidine hydrochloride monohydrate
5-(3-dimethylaminopropyl)-10,11-dihydrodibenzo(a,d)cyclohepten-5-ol
1,2,5,6-tetrahydropyridine-3-carboxylic acid
2-ETHYL-4-METHYLIMIDAZOLE
SARCOPHYTOL A
N-Benzoylcytidine
3,5-Diphenyl-2-thioxo-2,3-dihydrothieno[2,3-d]pyriMidin-4(1H)-one, 96%
3-(TrifluoroMethoxy)benzyl alcohol, 98+%
2,3,4-Trifluorophenol
5-Chloro-2-fluoroaniline
9-Phenanthreneboronic Acid (contains varying aMounts of Anhydride)
Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
DiallylaMine
4-tert-Butyl-2,6-diisopropylphenol
3-Chloro-2,6-difluorobenzoyl chloride
2,6-Diisopropylphenol ,99%
2,6-Dichlorotoluene, 99+%
3-Methoxypyridine-5-boronic acid pinacol ester
AMINOGUANIDINE HYDROCHLORIDE
ZINCON
2,3,4,5,6-PENTAFLUOROPHENOXYACETYL CHLORIDE
Neodymium trifluoride
3-Nitrotoluene
1-Butyl-3-MethyliMidazoliuM thiocyanate
L-Leucyl-L-tyrosine
N-(5-BroMopyridin-2-yl)pyrrolidine
(4-Methyl-2-phenyl-1,3-thiazol-5-yl)Methanol, 97%
ISOPROPYL BUTYRATE
Tetrakis(1,1,1,3,3,3-hexafluoroisopropyl) Orthosilicate
piperidine-4-propanol
[1S-(1α,4aα,6α,7α,7aα)]-1-(β-D-glucopyranosyloxy)-1,4a,5,6,7,7a-hexahydro-6-hydroxy-7-methylcyclopenta[c]pyran-4-carboxylic acid
4-(prop-2-en-1-yloxy)phenol
Benzyl glycidyl ether
BOC-D-3,4-Dichlorophe
RubidiuM fluoride
4,4'-ISOPROPYLIDENEDIPHENOXYACETIC ACID
1-(4-Chlorophenyl)-1-cyclobutanecarbonitrile
2-Azidotoluene solution
1-Naphthalenemethylamine, 97%
3-Methoxyphenol, 97%
(Chloromethyl) phenyl ethyl dimethylchlorosilane
Chromone-3-carboxylic acid, 95%
2-Ethylfuran
3-Ethylpyridine
N,N-DI-N-BUTYLETHYLENEDIAMINE
Ethyl N-benzyl-3-oxo-4-piperidine-carboxylate hydrochloride
1-BroMoadaMantane
2-(4-Nitrophenyl)-4,4,5,5-tetraMethyliMidazoline-3-oxide-1-oxyl Free Radical
3-(4-BroMophenyl)-1,2,4-oxadiazol-5(4H)-one
SodiuM ethoxide, 96%, pure
Loratadine, USP
2-Hydroxy-3-Methyl-2-cyclopentenone
3-Fluoro-4-nitrobenzoic acid
Methyl 4-amino-3-nitrobenzoate
3-chloropentane
Formanilide
STK ELISA Kit
S100B ELISA Kit
Human PCDHα8 ELISA Kit
Human DNM2 ELISA Kit
Chicken L-Phenylalanine ammonla-lyase,PAL ELISA Kit
Rat GLβ (Galactosidase, Beta) ELISA Kit
sE-selectin ELISA Kit
Human Elastin,ELN ELISA Kit
HSA ELISA Kit
APT ELISA Kit
Sodium Sulfite Agar
T1N3 Broth
Dimethyl acetylsuccinate
Nickel(II) fluoride, anhydrous, 97%
5,5-DiMethylhydantoin, 97%
2-Fluoro-3-nitropyridine
o-Tolylacetic acid
Ammonium perrhenate(VII)
Oryzanol
2,6,6-trimethylcyclohepta-2,4-dien-1-one

Trimethylboroxine
N-(4-HYDROXYPHENYL)METHACRYLAMIDE
3-Chlorocinnamic acid
N^a-Boc-N^d-FMoc-L-ornithine, 95%
4-(3-Pyrrolidinyl)Morpholine, 97%
D-(+)-Xylose
Diphenylphosphine oxide
2-Mercapto-5-Methoxythiazolo[4,5-b]pyridine
1,4-DIIODO-2,3,5,6-TETRAMETHYLBENZENE
(S)-(+)-2-Hexanol
DODECYL ACETATE
2-Benzyl-4-chlorophenol , 97%
Fumaramide, 98%
Ethyl 2-amino-1,3-oxazole-5-carboxylate 95%
2,3,6-Trifluorophenylacetonitrile