RPC细胞：大鼠垂体复苏细胞系更多低价

RPC细胞：大鼠垂体复苏细胞系更多低价 货号：GD-C5819725
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞，说一点自己培养细胞方面的看法：1)培养细胞前，可以看看细胞特点说明书，包括细胞正常生长的状态图、传代、培养基的类型等；2)不同细胞生长周期不同，有的生长很快，比如说MDA-MB-231，传代的时候取离心悬液的十分之一就已经足够了，有的又是特别慢，等的人心焦，BT474就是，传代是一分二，复苏起始的时候两周多才能长满，所以就要在培养细胞的过程中多多摸索了；3)对于娇弱的细胞，就得细心呵护了，胰酶消化不能过久，吹得时候要轻柔，离心时候也要注意转速和时间，每天都要去看一下细胞，肉眼观察培养基状况、显微镜下观察细胞生长状况；4)冻存细胞复苏后第二天Z好更换一次培养基；5)培养箱隔一段时间彻底用酒精棉擦洗一次；6)养细胞的教训：不能偷懒!细胞虐我千百遍，我待细胞如初恋。1)不管买的细胞、惠赠的细胞，刚拿到手赶紧做两件事：检测是否有支原体污染; 迅速扩增冻存；2)发现有污染迅速处理掉，特别是当你养了很多种细胞时; 细菌污染，真菌污染很容易发现，支原体污染的话，细胞会长的慢，买个支原体检测的kit定期检测一下；3)传代细胞日常的值日清洁是必须的，此外还要定期熏蒸；4)细胞的传代一定要规律，保持相同的confluency和传代时间间隔，不要随心所欲或者拖延；5)注意个人卫生。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
EthynyltriMethylsilane
Fenoxaprop-p-ethyl
2-Acetylbenzofuran
4-Butoxy-3-chlorophenylboronic acid
Tolylene 2,5-diisocyanate
3-BroMo-5-isopropoxyphenylboronic acid, pinacol ester
1-Methylpiperidine-4-carboxylic acid hydrochloride
Bindschedler's Green Leuco Base
Tris(3,5-dimethylphenyl)phosphine, 98%
3-(Cyclopentadienylpropyl)triethoxysilane (dimer)
1-Chloro-1-fluoroethane
4-Iodobenzyl bromide
5-AMINO-2-NITROBENZOIC ACID
1,2,3,4-Tetrachlorobenzene
3-(Phenylthio)thiophene
2,3,4-TriMethoxybenzaldehyde
5,5'-DibroMo-2,2'-bipyridyl
2,6-DiforMyl-3,5-diMethoxyphenol
TetraethylaMMoniuM p-toluenesulfonate, 98%
1,3-BUTADIENE
2-Ethoxyethyl Cyanoacetate
Neryl butyrate
3,6-Dihydroxynaphthalene-2,7-disulfonic acid, disodium salt
2,4-Hexadienyl acetate
5-Fluorotryptamine
5-Bromo-2-nitropyridine
N-Boc-N'-Cbz-L-lysine
3-BroMothiophene
7-BroMo-1,2,3,4-tetrahydroisoquinoline hydrochloride, 95%
3-Mercapto-2-pentanone, 98%, stab. with 0.1% CalciuM carbonate
5-Methyl-1H-tetrazole
n-Dodecyl α-D-Maltoside
N-Benzyl 3-boronobenzenesulfonaMide
NEODYMIUM SULFATE
2,3-Dichloropropene
4-(1H-Tetrazol-5-yl)benzaldehyde, 97%
Tris(isopropylcyclopentadienyl)cerium
2-(ethylsulfonyl)ethanamine(SALTDATA: HCl)
1,1'-Oxalyldiimidazole
Ethyl 4-[(4-Methoxybenzylidene)amino]cinnamate
1-(3-Aminopropyl)pyrrolidine
4-Piperazinobenzonitrile
METHYL 3-INDOLYLACETATE
AdeMetionine Disulfate Tosylate
n-Butyl disulfide
9-AMinocaMptothecin
Xylazine
4-Chloro-3-Methylphenol, 99+%
5-Methyl-1-benzothiophene-2-carbaldehyde, 97%
2-Methyl-2-butene
2-ThiophenesulfonaMide
S-triazine (1,3,5)
4-Pyridinecarboxamide,3-fluoro-
1,3-Bis(3-Methacryloxy-2-Hydroxypropoxypropyl)Tetramethyldisiloxane
3-Amino-4-chloroacetanilide
1-Dimethylamino-2-propanol
DL-1-Phenethylalcohol
4,5-Difluoro-2-Methoxyphenylboronic acid
3-(DICYANOMETHYLIDENE)INDAN-1-ONE
DIMETHYL BROMOMALONATE
Methyl 3-bromothiophene-2-carboxylate , 97%
2-Fluoropyridine-4-boronic acid, pinacol ester
[2-(4-Methoxy-phenyl)-5-oxo-cyclopent-1-enyl]-acetic acid
2,3-Difluorophenylisothiocyanate
4-Bromo-o-xylene
N-ACETYL-DL-NORLEUCINE
Disodium citrate
PhosphoMolybdic acid hydrate
1-Phenyl-2-thiourea
1,3-Di-O-benzyl-2-O-lauroylglycerol
N-Isopropyl 2-broMo-5-fluorobenzaMide
TriMethylsilylacetylene, 98%
PyBOP
N,N'-Bis(diMethylaMinoMethylene)hydrazine
5-Nitrouracil
3-(CYANOMETHYL)BENZOIC ACID
4,5-dichlorophthalic anhydride
1,1,1-trifluoro-2-butanone
HSP-70 ELISA Kit
Flt3 ELISA Kit
Human hnRNP A1 ELISA Kit
Human DRD1 ELISA Kit
BMPR2 ELISA Kit
Rat Interleukin 15,IL-15 ELISA Kit
TGF-β1 ELISA Kit
PR3-ANCA ELISA Kit
Human PDGFRα ELISA Kit
TIG ELISA Kit
Campy-Cefex Agar Medium Base
Campy-Cefex Agar Medium Base
LithiuM Acetate Dihydrate
trans-N-Boc-4-cyano-L-proline, 97%
4-n-Propoxypiperidine, 95%
2-Phenoxybiphenyl, 98+%
Neryl butyrate
5-Methyl-2-aminobenzothiazole
Ethyl 3-methylthiopropionate
2,7-dimethylnaphthalene

3-NITROBIPHENYL
2-AMino-4-Hydroxypyridine

4,4',4''-TriMethoxytrityl alcohol, 95%
N-Boc-3-benzyloxyMethyl-L-histidine, 98%

3-TERT-BUTYLTOLUENE
ThioMorpholine
2-Hydroxyisonicotinic acid
4-n-Butylphenyl isocyanate, 98%
Bis(2-Methacryloyl)oxyethyl disulfide
4-(Piperidin-1-ylmethyl)benzaldehyde 95%
4-(Trifluoromethoxy)phenol
Ethyl pipecolinate, 98+%
1-Methyl-2-phenylethyl Acetate