GIST-T1细胞：人胃肠道间质瘤复苏细胞系更多折扣

GIST-T1细胞：人胃肠道间质瘤复苏细胞系更多折扣 货号：GD-C5918797

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-(MethylcarbaMoyl)pyridine-5-boronic acid pinacol ester, 96%
4-Fluoro-4'-hydroxybiphenyl
0
Methyl 1-Methoxybicyclo[2.2.2]oct-5-ene-2-carboxylate
4,4'-DithiodiMorpholine
Z-Cys(Z)-OH
ETHYL PERFLUORONONANOATE
7-methyl-1,3-dihydro-2H-indol-2-one(SALTDATA: FREE)
N,N-Diethyl-2-piperidinecarboxamide hydrochloride
Mercury(II) selenide (99.99%-Hg) PURATREM
Methyl 3-hexenoate, predominantly trans, 95+%
FMOC-D-2-Chlorophe
ETHION
2,6-DIISOPROPYLPHENYL ISOTHIOCYANATE
2-Fluorobenzenesulfonyl chloride
Methyl 2,4-difluorobenzoate
4,4'-DiMethyl-trans-stilbene
3-BroMo-4-(trifluoroMethoxy)phenol
TetrabroMothiophene, 99+%
N,N-MethylenebisacrylaMide, Electrophoresis Grade
Ethyl 2-(5,5-DiMethyl-1,3,2-dioxaborinan-2-yl)benzoate
barium oxalate
3-alpha-hydroxy-7-oxo-5-beta-cholan-24-oic acid
1H,1H,2H,2H-Perfluorodecan-1-ol
Ethyl m-toluate
2,5-Hexanediol
Disperse Orange 25
2-[(DiphenylMethyl)Thio]AcetaMide
2-AMinopyridine-4-carboxaMide, 95%
N,N-DiMethylforMaMide diMethyl acetal, 97%
1-Naphthaleneacetic anhydride
N-Boc-cis-4-hydroxy-D-proline Methyl ester
3-BroMo-2-pyridinaMine
SODIUM PHTHALOCYANINE
3-Chloro-2-Methylaniline
Iodide Ionophore IV
2,6-Difluoromandelic acid
Phenylthiotrimethylsilane, 95%
Didecyldimethylammonium Bromide
2-Ethoxyethyl Trichloroacetate
NILE RED
Rizatriptan benzoate
Triethylvinylsilane
4-Acetylphenylboronic acid
5-(Benzyloxy)-2-broMo-4-Methylaniline
4,4'-Bis(2-Methoxystyryl)biphenyl
alpha-HexylcinnaMaldehyde
6-Fluoro-8-Methoxyquinoline
(?)-Citronellic acid, 95%
Garcinia cambogia extract
4-tert-Butylphenol
5-isopropyl-3-methylphenol
Disperse Blue 27
L-5-Hydroxytryptophan
1,3,5-Trivinyl-1,1,3,5,5-pentamethyltrisiloxane
Ethyl acetimidate hydrochloride
ASTRAZON BRILLIANT RED 4G
2-Isopropoxypyridine-3-boronic acid
5-(Ethoxycarbonyl)furan-2-boronic acid, pinacol ester
1,3-Dibenzylidene-2-cyclohexanone
4-Bromo-2-methylanisole
tert-Butyl 3-endo-hydroxy-8-azabicyclo[3.2.1]octane-8-carboxylate , 97%
1,3-Bis(2,4,6-trimethylphenyl)-4,5-dihydroimidazol-2-ylidene[2-(i-propoxy)-5-(N,N-dimethylaminosulfonyl)phenyl]methyleneruthenium(II) dichloride Zhan Catalyst-1B
1-Cyclopentenecarboxylic acid
DL-ETHIONINE
2-AMINOHEPTANOIC ACID
5,5-Diphenylhydantoin
4-(4-Chlorobenzoyl)pyridine
1,2,3,4-Cyclopentanetetracarboxylic Dianhydride
2-(3-MethoxypropylaMino)pyriMidine-5-boronic acid, pinacol ester
Ethyl MethylcarbaMate, 99%
2,4,6-Tris(trifluoroMethyl)-1,3,5-triazine, 99%
2-Furylboronic Acid (contains varying aMounts of Anhydride)
2-Sulfobenzoic Acid MonoaMMoniuM Salt
Tetraphenylphosphonium chloride
Triphenylmethyl Chloride
Ethanone, 1-[7-amino-5-(2-bromopropyl)-2,3-dihydro-1H-indol-1-yl]-
N-(3-MALEIMIDOPROPIONYL)BIOCYTIN
HO2 ELISA Kit
IL-13 ELISA Kit
Human ITIH3 ELISA Kit
Human CAM-ab ELISA Kit
Chicken IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit
DKK3 ELISA Kit
PCSK9 ELISA Kit
Human PSA-U S ELISA Kit
Human STAM1 ELISA Kit
RLT ELISA Kit
ISP Medium 1(Tryptone Yeast Extract Broth
Xylose lysine Desoxycholate Agar
Stearic Acid n-Butyl Ester
TitaniuM silicide, 99.5% (Metals basis)
5-Iodoindole, 98%
2-Methylbenzyl Mercaptan, 97%
barium oxalate
Bromelains
5-Phenyl-4H-[1,2,4]triazole-3-carboxylic acid
3-aminonaphthalene-1,5-disulphonic acid

4-Methyl-1-tetralone
2-METHYLINDOLE-3-ACETIC ACID
DenatoniuM benzoate
N-Boc-2-Methyl-D-serine Methyl ester, 97%
3-AMino-1H-1,2,4-triazole, 96%
4-AMinoMethyl-1-(n-butyl)piperidine
AMMoniuM iron(III) oxalate trihydrate
4-Fluorobenzoyl chloride
Boc-Ala-OSu
1,1,2,2-Tetrakis(4-Methylphenyl)-1,2-ethanediol
GLYCINE N-PROPYL ESTER HYDROCHLORIDE
3-Amino-2,6-dichloro-4-(trifluoromethyl)-pyridine
2,2,2-Trifluoroethyl methanesulphonate 98%
3-Methyl-1,3-benzoxazole-2(3H)-thione , 97%
Cobalt(II) hydroxide (97%-Co)