OUMS-27细胞：人软骨肉瘤复苏细胞系更多折扣

OUMS-27细胞：人软骨肉瘤复苏细胞系更多折扣 货号：GD-C5918752

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Methyl indole-3-carboxylate, 99%
FMoc-Tyr(PO3Bzl2)-OH
CesiuM fluoroaluMinate
Dichloro[1,3-Bis(2-Methylphenyl)-2-iMidazolidinylidene](benzylidene)(tricyclohexylphosphine)rutheniuM(II)
1-(2,4-DICHLORO-PHENYL)-ETHYLAMINE
2-AcetaMidopyridine-5-boronic acid
3-BROMO-2,4-DIMETHYL-5-PHENYLTHIOPHENE
3,4-Dihydro-2H-pyran
4-(4-Bromophenyl)thiophene-2-carboxylic acid
(4-Chloro-2-methylphenoxy)acetic acid hydrazide 98%
Dicyclohexyldichlorosilane
2-Anisidine-4-sulfonic acid
Dimethyl methylmalonate
4-(Methylmercapto)aniline
2-(Dicyclohexylphosphino)biphenyl
3-AMino-4-hydroxybenzonitrile
Ethyl 2,3-DibroMobutyrate
1-BroMo-3-tert-butylbenzene
Terephthalic acid, 99+%
Metronidazole Benzoate, Powder, BP
N-PhenylethylenediaMine
3,5,5-Trimethylhexanoic acid
5-Bromopyrimidine
methyl benzenesulfinate
3-carboxy-1-phenyl-5-pyrazolone
SELENIUM OXYCHLORIDE
METHYL THIOPHENE-3-ACETATE
1,3-Dithiane
5-Methyl-3-phenylisoxazole-4-carbonyl chloride, 99%
4,4'-Difluorobenzil, 99%
Dowex® 1X8 chloride forM
FMoc-Val-OH
1,1'-DIBROMOFERROCENE
SulfaMonoMethoxine
CadMiuM sulfide
4-Isopropoxy-2,3,5,6-tetrafluorophenylboronic acid
4-(Trifluoromethyl)benzonitrile 98%
(1R,2R)-2-Amino-1-(4-nitrophenyl)propane-1,3-diol, 96%
3-Thienylmagnesium iodide, 0.3M solution in THF, AcroSeal
Maltopentaose
N-(4-Aminobenzoyl)-beta-alanine
(3S)-3-(9H-Fluoren-9-ylmethoxycarbonylamino)-5-oxo-5-[tri(phenyl)methylamino]pentanoic acid
N-Phenyl-n-butylamine
CurcuMin; C.I. 75300;
4-Benzyloxyaniline hydrochloride
3-n-Hexadecylthiophene
PentafluorophenyldiMethylsilyldiethylaMine [PentafluorophenyldiMethylsilylating Agent]
(-)-1,2-Bis[(2R,5R)-2,5-diethylphospholano]benzene, 97%
TriMethyl(2-thienyl)silane, 97%
Hexafluorosilicic acid
5-BroMo-2-(Methylthio)pyriMidine-4-carboxylic Acid
N-Benzyl-4-carbethoxy-3-piperidone
Methylthiomethyltolylsulfone
Cinnamyl chloride
Holmium(III) oxide
Perfluorodecyl iodide
3-FLUORO-5-METHYLBENZOIC ACID
3,4,5,7-TetraMethoxyflavone, 97%
cis-2-Methyl-7-octadecene
3-Phosphonopropanoic acid
6-Methoxy-2-naphthylmagnesium bromide, 0.5M solution in THF, AcroSeal
1-Deoxy-1-nitro-D-galactitol, 99%
2'-Chloro-5'-nitroacetophenone
Geranyl linalool
Isopropylboronic acid
2,3-DIBROMO-3-PHENYLPROPIOPHENONE
N-Lauroylsarcosine sodiuM salt;SodiuM N-lauroylsarcosinate;Sarcosyl;Sarkosyl NL
5-Nitro-3-phenyl-1H-indole-2-carboxylic acid
1,1,1,2-Tetrafluoroethane
TetrabutylphosphoniuM Tetrafluoroborate
2-Chloropropionyl chloride, 95%
3-(Methylthio)propionaldehyde, 97%
Chloroquine diphosphate
Ethyl 3-(Methylthio)propionate
4-CYANOPHENYLZINC BROMIDE
4-Benzyloxy-3,5-dimethylbenzoic acid
2,3-Dimethylbenzylamine
Poly propylene glycol
TPO ELISA Kit
IL1β ELISA Kit
Human NAP ELISA Kit
Human CG ELISA Kit
Chicken Transforming Growth factor β1,TGF-β1 ELISA Kit
CX3CR1 ELISA Kit
HAVCR1 ELISA Kit
Human FOXO3 ELISA Kit
STAT5B ELISA Kit
RB1 ELISA Kit
Agrobacterium rhizogene Broth Medium
Skim Milk Medium
Potassium oleate
DiMethyl sebacate, 97%
5-Fluoro-2-nitrobenzonitrile, 97%
2-Methylanthraquinone
3,5,5-Trimethylhexanoic acid
Antimony pentafluoride
Di-T-Butoxydimethoxysilane
1,5-dihydroxy-4,8-dinitroanthraquinone

1-(4-METHOXYPHENYL)-1-CYCLOPROPANECARBOXYLIC ACID
POLY[4,5-DIFLUORO-2,2-BIS(TRIFLUOROMETHYL)-1,3-DIOXOLE-CO-TETRAFLUOROETHYLENE]
Indole-6-Carboxylic Acid
1,3-Dihydrobenzo[c]thiophene-5-thiocarboxaMide, 97%
Ethyl thiophene-2-glyoxylate, 97%
Gly-Gly-Gly
Isopropyl B-D-thioglucopyranoside
Cyclopropyl acetylene
2-BroMo-4-chlorotoluene
3-METHYLHEXYL ACETATE
4-(PIPERIDIN-4-YLOXY)BENZAMIDE
3,5-Dichloro-2,4,6-trifluorobenzoic acid
5-Bromo-furan-2-carboxylic acid
L-Valine, N-CBZ protected
1-Chloro-1,1-dibromo-2,2,2-trifluoroethane (FC-113aB2) 97%