RH30[RC13,RMS13,SJRH30]细胞：人横纹肌肉瘤复苏细胞系更多折扣

RH30[RC13,RMS13,SJRH30]细胞：人横纹肌肉瘤复苏细胞系更多折扣 货号：GD-C5918253

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
tert-Butyl (S)-1-Boc-5-oxopyrrolidine-2-carboxylate, 95%
2',4'-DiMethylacetophenone
2-(4-AMinophenyl)ethanol
TetrabutylaMMoniuM fluoride
MethyltriphenylphosphoniuM broMide
1,3,5,7-TetraMethyl-6-phenyl-2,4,8-trioxa-6-phosphaadaMantane
4-NITROPHENYLGLYOXYLIC ACID
1-(Carbamoylmethyl)pyridinium Chloride
9-Decenoic acid
(1-methyl-1H-benzimidazol-2-yl)methanol(SALTDATA: FREE)
5-Bromo-2-nitrophenol 98%
2,6-Dichlorobenzothiazole
ZINC CHROMATE
Praseodymium(III) nitrate hexahydrate
Methyl 1-(Mercaptomethyl)cyclopropaneacetate
Diethyl phosphonoacetaldehyde diethyl acetal
4,4'-Dipyridyl Sulfide
4-BroMoisoquinolin-1-ol
hexahydrate, 98%
EthylenediaMinetetraacetic Acid MagnesiuM DisodiuM Salt
1-(tert-Butoxycarbonyl)-2-piperidone
2-Ethoxybenzoic acid
m-chloroanisole
Sodium carbonate
Formic acid
1-IODO-2,3,5-TRICHLOROBENZENE
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt
4-Fluoro-N-(2-fluorophenyl)benzaMide, 97%
ZirconiuM(IV) n-propoxide, 70% w/w in n-propanol, packaged under Argon in resealable CheMSeal^t bottles
Pentafluoropyridine, 99%
AMMoniuM hypophosphite
Dibutyltin Maleate
BORON TRIFLUORIDE, N-PROPANOL REAGENT 15
γ-Phenyl-α-(trifluoroMethyl)-γ-butyrolactone,Mixture of cis and trans
2,6-DiMethoxybenzoyl chloride
EthylenediaMinetetraacetic acid dipotassiuM salt dihydrate
2-Ethoxycarbonylbenzeneboronic acid
4-(2-Bromoacetyl)benzonitrile
Phenylmalonic Acid
Azelanitrile
2-Mercapto-3-butanol
2-CHLORO-4-NITROBENZOYL CHLORIDE
BIS(2,2,2-TRIFLUOROETHYL) ETHER
TaMoxifen;(Z)-2-[4-(1, 2-Diphenyl-1-butenyl)-phenoxy] -N, N- diMethylethanaMine;1-p-β-DiMethylaMinoethoxyphenyl-trans-1,2-diphenylbut-1-ene
3-Chloro-2-Methylphenyl isothiocyanate
3-BenzalbutyraMide
N-Methoxy-N-MethylbutanaMide
6-Chlorooxindole, 98%
2-Chloro-6-Methoxy-3-nitropyridine, 97%
4-tert-Butylcyclohexanol (cis- and trans- Mixture)
2-Chloro-1-MethylpyridiniuM p-Toluenesulfonate
Lithium benzoate
4-nitrophenyl-β-D-galactopyranoside
Rose Hip Oil
N,N,N',N'-Tetraethyl-1,3-propanediamine
2,3,5-Ttrifluorobenzaldehyde
4-(4-DiMethylaMinophenylazo)phenylarsonic Acid Hydrochloride , 95%
Benzenesulfonic acid
Crotononitrile (pract)
Itaconic Anhydride
D-Glucose ,98%
DL-3-Methyl-2-butanol, 98%
2'-methoxybiphenyl-3-carboxylic acid(SALTDATA: FREE)
Zileuton
5-CHLORO-2-METHOXYANILINE HYDROCHLORIDE
3-BROMO-4-(TRIFLUOROMETHOXY)ANILINE
Kryptofix 222
cis-2-(tert-ButoxycarbonylaMino)cyclohexanecarboxylic acid
(-)-(R)-N,N-DiMethyl-1-[(S)-1′,2-bis(diphenylphosphino
2-TriMethylsilyl-1,3-dithiane
2,9-DiMethyl-4,7-diphenyl-1,10-phenanthroline, 98%
PotassiuM tetracyanoplatinate(II) trihydrate
FLUORESCEIN DIACETATE
DAPT
D-Luciferin Sodium Salt
Boc-N-methyl-D-leucine
2-Amino-1,3-thiazole-5-carboxylic Acid
8-Methoxyquinolin-2(1H)-one
TFPI ELISA Kit
Mouse FOXP3 (Forkhead Box Protein P3) ELISA Kit
tPA ELISA Kit
BGP ELISA Kit
PⅢNP ELISA Kit
Apramycin ELISA Kit
AT-1 ELISA Kit
Human CSRP1 ELISA Kit
Human Surface Membrane Immunoglobulin,SmIg ELISA Kit
Anti-AChR ELISA Kit
Fluid Soybean-Casein Digest Broth
Sterile penicillin solution
Zoledronic acid hydrate
SulfaMethoxazole
5-BroMo-2-pyridineacetic acid, 98%
2-Cyano-3-iodopyridine, 95%
2-Ethoxybenzoic acid
methyl heineicosanoate
2-Ethylbutyl methacrylate
2-Chlorobutane

Gibberellic acid
ACID RED 66
N-alpha-(tert-Butoxycarbonyl)-L-lysine
3,5-Bis(Methoxycarbonyl)benzeneboronic acid, 97%
Acetylcholine chloride, 98+%
CarboxyMethyl cellulose
Erlose
2-CYCLOHEXEN-1-ONE DIMETHYLKETAL
4-(HydroxyMethyl)phenylboronic acid
2,6-DICHLOROBENZYLIDENEACETONE
N,N'-DI-TERT-BUTYLETHYLENEDIAMINE
4-aminotetrahydrothiophene-3-ol 1,1-dioxide(SALTDATA: FREE)
(-)-Dibenzoyl-L-tartaric acid monohydrate, 98%
4-Piperazin-1-ylbenzaldehyde
1-Aminoanthracene