AtT20细胞：小鼠垂体瘤复苏细胞系更多低价

AtT20细胞：小鼠垂体瘤复苏细胞系更多低价 货号：GD-C5820503
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Iron TitaniuM powder, -40 Mesh
Kinoprene
DihydroarteMisinin , 98%
Methyl (4S,5S)-dihydro-5-Methyl-2-phenyl-4-oxazolecarboxylate
1,1-Di-tert-butoxytriMethylaMine
4-(N-AcetylsulfaMoyl)phenylboronic acid
3-Azidoanisole solution
Dansyl Fluoride
Hexafluoro-1,2,3,4-tetrachlorobutane
Fluorolube grease GR 290
(S)-beta-(p-methylphenyl)alanine
N-Acetyl-L-asparagine
PYRIDINE-2-CARBOXAMIDE
Adipoyl chloride
1,2,3,4-Butanetetracarboxylic acid
3-Chlorothiophene-2-carbaldehyde
Docetaxel
(4-BroMophenyl)acetaldehyde
2,3-DiMethylbenzoic acid, 98%
ThyMidine, Reagent, High Purity
7-Hydroxy-4-MethylcouMarin-3-acetic Acid
δ-Decalactone
1,2,3,4-Tetrahydro-1,1,4,4-tetramethylnaphthalene
Iron(III) oxide
heptadecanoic acid
alpha-Amylase
IBOTENIC ACID
sec-Butanol
N-Boc-trans-4-hydroxy-D-proline, 99%
3-(TrifluoroMethoxy)benzonitrile, 97%
(±)-2-AMino-1-butanol
o-Toluic acid
5,10,15,20-TETRAPHENYL-21H,23H-PORPHINE VANADIUM(IV) OXIDE
N-N-VALERYL-D-GLUCOSAMINE
(±)-2-Chlorophenylglycine
4,5-DIMETHOXY-1,2-PHENYLENEDIAMINE DIHYDROCHLORIDE
(2S,3S)-trans-3-(Carboxymethyl)-azetidine-2-acetic acid, 98%
1,2-Dichlorotetrafluorocyclobut-1-ene 97%
8-Methyl-8-azabicyclo[3.2.1]octan-3-amine
Di-mu-chlorobis[5-hydroxy-2-[1-(hydroxyimino)ethyl]phenyl]palladium(II) Dimer
O-Phospho-L-serine
4,5-Dichloro-2-octyl-isothiazolone
ETHYL 3-(2-FURYL)PROPIONATE
Isopropyl chloroformate
1-(2-AMinoethyl)iMidazolidin-2-one
N-Boc-DL-pipecolinic acid
Monoelaidin
2,3-DiaMino-5-broMo-6-picoline
2,3-Dichlorothiophene, 97%
5,7-Diiodo-8-quinolinol
DL-Alanyl-L-alanine
2,3-dihydro-1H-inden-2-yl(Methyl)aMine(HCl)
5,6-dimethylbenzimidazol-2-ylamine
tween(R) 65
Trisiodophenylamine
Diphosphoryl chloride
4-AMino-2,6-dichloropyriMidine
(+)-DI-TERT-BUTYL L-TARTRATE
RhodiuM octanoate diMer
2,15-Hexadecanedione
Methyl 2,5-dihydro-2,5-dimethoxy-2-furancarboxylate, tech.,mixture of cis and trans, 85%
4-Chloro-5-fluoro-7H-pyrrolo[2,3-d]pyrimidine
Ethyl trans-2-amino-4-cyclohexene-1-carboxylate hydrochloride, 98%
Hemin
5-(Aminosulfonyl)-3-chloro-1-methyl-1H-pyrazole-4-carboxylic acid ethyl ester
3-Ethoxy-4-methoxybenzaldehyde
3-Bromonitrobenzene
2-(2-hydroxyethoxy)ethyl acetate
1-Cyclohexyl-2-buten-1-ol (cis- and trans- Mixture)
4-Propoxy-2-Methylphenylboronic acid
Hydridotetrakis(triphenylphosphine)rhodiuM(I)
4-IsopropylcinnaMic acid, 98%
SodiuM Isonicotinate
GiMeracil
ethyl phenoxyacetate
Hyoscyamine
6-OH-BTA-0
[(R)-(+)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl]palladium(II) chloride
GHRP ELISA Kit
NT-proBNP ELISA Kit
FT4 ELISA Kit
Human OTOR ELISA Kit
C3 ELISA Kit
bTC ELISA Kit
Chicken NT-3 (Neurotrophin-3) ELISA Kit
PKB ELISA Kit
TSP-1 ELISA Kit
CENPA ELISA Kit
Streptococcus mutans Medium
Bile Esculin Agar
Diethyl AcetaMidoMalonate
4-Chloro-N-Furfuryl-5-SulfaMoylanthranilic Acid
5-(2-Thienyl)isoxazole-3-carboxylic acid, 95%
2-Iodothioanisole, 96%
δ-Decalactone
2-Amino-5-ethyl-1,3,4-thiadiazole
Solvent Blue 70
Allicin

Trichloropropylsilane
AMMONIUM ADIPATE
Ethyl ziraM
3-Carboxy-PROXYL, free radical, 98%
ThyMine, 97%
N-VanillylnonanaMide
SodiuM 4-vinylbenzenesulfonate
N-METHYL-4-(2-CHLOROACETAMIDO)BENZOIC ACID
Triphenylphosphine-3,3',3''-trisulfonic acid trisodiuM salt
2,3,4-TRIFLUOROPHENYL ISOCYANATE
Octadecy triMethyl aMMoniuM broMide
(R,R)-(-)-2,5-Bis(methoxymethyl)pyrrolidine, 96%
Methyl 4-bromo-1-benzothiophene-2-carboxylate
(R)-5-Allyl-2-oxabicyclo[3.3.0]oct-8-ene
Isothiazole-5-carboxylic acid