ECA细胞：小鼠艾氏腹水瘤复苏细胞系更多低价

ECA细胞：小鼠艾氏腹水瘤复苏细胞系更多低价 货号：GD-C5819296

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Tri-n-butylgerManiuM hydride, 97+%
4-BroMosalicylic acid
Benzothiazole, 5-chloro-2-(Methylthio)- (7CI,8CI,9CI)
1-PHENYLUNDECANE
2,4,6-TriMethylphenol
L-Cysteinesulfinic Acid, Monohydrate
2,3,4,5,6-PENTABROMOTOLUENE
Bromoethyltriflate
2-N-Boc-Amino-benzothiazole-6-carboxylic acid
2-methyl-1,3-oxazole-4-carbonitrile(SALTDATA: FREE)
Azobenzene-3,3'-dicarboxylic Acid
Cobaltous nitrate hexahydrate
1-Phenylpropan-1-amine
1,3-Bis[tris(hydroxymethyl)methylamino]propane
4-BroMoMethyl-3-nitrobenzoic acid
L(+)-Leucinol
Diethyl 2,5-DibroMoterephthalate
Methyl 2-broMo-3-nitrobenzoate
5-Methyl-DL-tryptophan, 98%
Terbutaline Sulfate, USP
Triisopropyl OrthoforMate
3-Fluorobenzenesulfonyl chloride
acid red 54
sodium hydrogen oxalate
Epsilon-Caprolactone monomer
Ethyl 1-benzyl-4-oxo-3-piperidinecarboxylate hydrochloride
5-NITROSO-8-HYDROXYQUINOLINE
6-BroMo-2-Chloro-Quinoline
2,6-DibroMo-3-chloro-4-fluoroaniline, 97%
(^+)-CaMphoric anhydride, 98%
N-(L-Phenylalanyl)-2-aMinoacridone
Isovaleric acid
2-FLUORO-3-(TRIFLUOROMETHYL)BENZOPHENONE
N-t-Butyl-1,3-propanesultaM
2-AMino-6-fluorobenzoic acid
VINYLMETHYLBIS(TRIMETHYLSILOXY)SILANE
Tetrabutylammonium hydrogen sulfate ,98%
2-Bromobenzotrifluoride
3-(3-methylphenyl)-1-propanamine(SALTDATA: FREE)
4,6-Dimethyl-2-(methylthio)pyrimidine ,98%
4-CHLORO-5-PHENYLTHIENO[2,3-D]PYRIMIDINE
Methyl carbazate
3-Methylbenzyl cyanide
o-Cresol;o-Hydroxytoluene;o-Cresylic alcohol;2-Methylphenol;o-Oxytoluene;
2-(2-Chlorophenoxy)ethaniMidaMide hydrochloride
2-Methoxythioanisole
(R)-(-)-1-(2-PyrrolidinylMethyl)pyrrolidine
1,2-Dichloropropane, 98%, pure
N1-(4-AMino-2-Methylphenyl)acetaMide, 90%
Phenidone
4-Methylcyclohexanecarboxylic Acid (cis- and trans- Mixture)
Methyl 2-amino-3-nitrobenzoate
Methyl 4-hydroxynicotinate
N-(2-Acetamide)iminodiacetic acid
4-Methoxytriphenylmethyl chloride
Lithium acetylide ethylenediamine complex
3-BroMo-2-hydroxyMethylpyridine
5-CHLORO-2-FLUOROBENZYLAMINE
2-NaphthaleneMethanol
2-Amino-4-(trifluoromethyl)oxazole 98%
cis,cis-9,12-Octadecadien-1-ol
3'-(Chloromethyl)-4'-methoxyacetophenone
N-Carbobenzoxy-DL-aspartic Acid
ALLYLDICHLOROMETHYLSILANE
2-Methyl-2-pentenal
2-CHLORO-4,6-DIFLUOROANILINE
Praseodymium(III) nitrate hexahydrate
Cyclohexyl isocyanide
1-Butyl-3-MethylpyridiniuM Chloride
4,4-DiMethylcyclohexen-1-ylboronic acid, pinacol ester
D(+)-alpha-MethylbenzylaMine, 99+%, (99% ee)
Nepsilon-Tosyl-L-lysine, 98%
p-Xylene-2-sulfonic Acid Hydrate
Phthalanilide
2-Thienylacrylic acid
3-Methyl-3-Pyrrolidinol hydrochloride
(1S,4Z,7S,10S,11E,20R)-4-ethylidene-7,20-dipropan-2-yl-9-oxa-15,16-dit hia-3,6,18,21-tetrazabicyclo[8.7.6]tricos-11-ene-2,5,8,19,22-pentone
trans,trans-4-(4-Fluorophenyl)-4'-propylbicyclohexyl
CYPD ELISA Kit
PDCN ELISA Kit
Human PRM1 ELISA Kit
Human DPYSL3 ELISA Kit
1,3-βD-Glu ELISA Kit
Rat β-actin (Beta actin) ELISA Kit
IgM ELISA Kit
Human PCMT1 ELISA Kit
Human HFE ELISA Kit
EP ELISA Kit
Starch Medium
MR-VP Medium
LanthanuM Acetylacetonate Hydrate
GadoliniuM(III) fluoride, anhydrous, 99.9% (REO)
5-(FMoc-aMino)-2-chlorobenzoic acid, 97%
Methyl 2-diphenylphosphino-1-naphthoate, 98%
3-Fluorobenzenesulfonyl chloride
Ethylsulfonylacetonitrile
Prasedymium(III) oxide
4-Isopropyl Benzoic Acid

ETHYL 4-(BENZYLOXY)BENZOATE
Levulinic acid
DL-Leucine
1-(3-BroMophenylsulfonyl)pyrrolidine, 97%
3',4'-Dichloropropiophenone, 98%
Diphenyl sulfide
1-Methylpyrazole
2-BroMo-4,5-dichloro-1H-iMidazole
AluMinuM Metaphosphate, Al2O3 19.4%, P2O5 78.9%
1,1,1-Tris(diphenylphosphinoMethyl)ethane
HEXAPHENYLBENZENE
4-Chloro-3-fluoroanisole
3,5-Dibromobenzo[b]furan
Methyl-2-bromo-6-chlorobenzoate
4-N-BOC-Amino-4-carboxytetrahydrothiopyran, 95%