HUT 102细胞:人T淋巴瘤复苏细胞系更多低价 货号:GD-C5819281
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Oxalic dihydrazide, 98%
Fenbuconazol
Tetraiodoethylene
ALPHA-D-GLUCOSE-1-PHOSPHATE NA2-SALT
2,7-DibroMofluorene
RubidiuM chloride
SODIUM METABORATE TETRAHYDRATE
2,4-Di-tert-butylphenol, 97%
Di-2-ethylhexylamine, 99%
N-Methyl-3-(trifluoromethyl)aniline
Diphenylammonium Trifluoromethanesulfonate
Carbazole-9-ethanol
3',4',7-Trihydroxyisoflavone
1,2,3,4-Tetrahydro-1-naphthol
5-BroMo-1-benzothiophene
Dicyclopentadiene (stabilized with BHT)
1,4-DibroMo-2,5-bis(broMoMethyl)benzene
Methyl 2-broMo-6-Methylbenzoate
(E)-3-Acetoxy-1-propenylboronic acid pinacol ester, 97%
SuMatriptan Succinate, EP
Methyl 4-(AMinoMethyl)benzoate Hydrochloride
2,3,5,6-Tetrafluoroaniline
Pigment Violet 23
cholesteryl N-decylate crystalline
1-Chloro-2-fluoro-4-methoxybenzene
CYCLOHEXYL ISOTHIOCYANATE
1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE
2-AMino-6-Chloropyridine
n-Dodecyltriethoxysilane, 95%
1-AMino-4-cyclopentyl, 97%
ZirconiuM carbide
cis-10-Heptadecenoic acid
3-BroMo-2-butoxy-5-fluorophenylboronic acid
N-Z-4-pentyne-1-aMine
6-broMo-4,4-diMethyl-3,4-dihydroquinolin-2(1H)-one
Di-μ-chlorodiMethylbis(triphenylphosphine)dipalladiuM
5-(Chloromethyl)-2-(1H-pyrazol-1-yl)pyridine ,97%
(4-fluorobenzyl)propylamine(SALTDATA: HCl)
5-Bromo-5-deoxy-2,3-O-isopropylidene-D-ribono-1,4-lactone
2-Mercaptopyridine-N-oxide, sodium salt monohydrate, 98%
Acid Blue 83
2,3,6-Trifluorophenylboronic acid
2-Formylcinnamic acid
1-PENTANESULFONIC ACID SODIUM SALT MONOHYDRATE
4-BroMo-2,5-difluorobenzenesulfonyl chloride
PhosphoMolybdic acid hydrate
ThiaMine Disulfide Nitrate
2,2'-DibroMobiphenyl, 98%
4-BroMo-2-chlorotoluene, 98%
1,1'-Oxydi-2-propanol
3,3-Difluoropyrrolidine Hydrochloride
tris(2,6-dimethoxyphenyl)phosphine
2'-O-t-Butyldimethylsilyl-5'-O-(4,4'-dimethoxytrityl)-N4-benzyl cytidine
N-Trimethoxysilylpropyl-N,N,N-trimethylammonium chloride
3,5-Dimethoxybenzonitrile
4-Amino-2,6-dichloropyrimidine
2-AMino-5-Methylthiophene-3-carbonitrile
(3S,4R)-4-(4-Fluorophenyl)-3-hydroxyMethyl-1-Methylpiperidine
AMMoniuM cupric chloride dihydrate
cis-4-Octene
4-Nitrophenyl 2-Acetamido-2-deoxy-beta-D-glucopyranoside
1-(4-(2-Aminoethyl)piperazin-1-yl)ethanone
2-(2-Benzothiazolyl)-3,5-diphenyltetrazolium Bromide
4-N-Boc-aminocyclohexanone
5-Chloro-2-nitrobenzotrifluoride
Allyl ether
1,3-BIS(ISOCYANATOMETHYL)CYCLOHEXANE
Isobutyl Mercaptan
1-Butyl-2-pyrrolidone
Ethyl 5-cyclopropyl-2H-pyrazole-3-carboxylate
Acridine hydrochloride, 97%
5-BroMo-1-Methyl-1H-indole-3-carbaldehyde, 97%
2-Phenylpropylene Oxide
4-(1-Naphthylvinyl)pyridine
alpha-Angelica lactone
9H-fluoren-9-ylmethyl piperidin-4-ylcarbamate hydrochloride
2-(5-Methylthiophen-2-yl)glycine
Trisilane, 1,1,1,3,3,3-hexachloro-2-(trichlorosilyl)-
Porcine Leptin,LEP ELISA Kit
Mouse P-Selectin ELISA Kit
Human PCDH11 ELISA Kit
Human DPP4 ELISA Kit
5HT/ST ELISA Kit
Rat β-Defensins,β-DF ELISA Kit
Chicken Immune RNA,Irna ELISA Kit
Human PC ELISA Kit
Human SAP ELISA Kit
HP-IgY ELISA Kit
Double-arginine Hydrolase Test Medium
Casein Digest Soybean Agar for Pzase Test
Lead(II) 2,4-pentanedionate
Potassium fluoride dihydrate
5-(Tri-n-butylstannyl)pyriMidine, 96%
2-Furaldehyde, 98%
2,3,5,6-Tetrafluoroaniline
1,1-Dimethylthio-2-nitroethylene
Tellurium dioxide
Hexadecanedioic acid
2,2'-Dithiosalicylic acid
MUCIC ACID
Iron(Iii) Sulfate N-Hydrate
Methyl 3-broMo-2-fluorobenzoate, 98%
6-AMinocouMarin hydrochloride, 97%
Boc-L-Lys(FMoc)-OH
2'-Deoxyuridine
Ethyl (4-trifluoroMethylbenzoyl)acetate
1-BROMO-3,4-DICHLOROBUTANE
Methyl 3,4-diMethylbenzoate
4-N-OCTYLANILINE
3-methyl-1-phenyl(SALTDATA: HCl)
Tetraoctadecyloxysilane
D-2-Allylglycine Hydrochloride
Tetramethyl 1,1,2,3-Propanetetracarboxylate