SGC-7901细胞：人胃腺癌复苏细胞系更多低价

SGC-7901细胞：人胃腺癌复苏细胞系更多低价 货号：GD-C5818909

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(4R)-(+)-4-Isopropyl-2-oxazolidinone, 98+%
2,3-DiMethyl-4-Methoxybenzaldehyde
2-Methyl-4-o-tolylbut-3-yn-2-ol
4-BroMophthaliMide , 98%
2-(4,5-Difluoro-2-nitrophenyl)acetic acid
6-Methylnicotinaldehyde
(1S,3S,5S)-2-Azabicyclo[3,3,0]octane-3-carborylic acid benzyl ester hydrochloride
Poly(ethylene glycol methyl ether), average M.W. 750
Sodium 4-Ethylbenzenesulfonate
1,5-Dibromo-2,6-dimethoxynaphthalene
Dichloro{(1S,2S)-N,N-bis[2-(diphenylphosphino)benzyl]cyclohexane-1,2-diamine}ruthenium(II), min. 97%
1-NAPHTHYLAMINE-3,6,8-TRISULFONIC ACID DISODIUM SALT HYDRATE
Thiazole-4-carboxaldehyde
4-ETHOXYBENZONITRILE
N-(Diphenylmethylene)aminoacetonitrile
2,3,4-Trifluoro-6-nitroaniline
1,1'-Dibutyrylferrocene
2,6-DibroMo-4-chloropyridine
ThyMidine, 99+%
PalladiuM (II) Acetate
Basic Blue 24
2-Nitrobenzoic Acid
O-Acetyl-L-carnitine hydrochloride
4-phenyl-1-butene
4-ACETOXYBIPHENYL
2,4-Dichlorobenzotrifluoride
2-Fluorophenol
5-Phenyltetrazole
GerManiuM(IV) iodide, 99.999% (Metals basis)
3-(TrifluoroMethyl)anisole, 99%
1-BroMo-4-iodobenzene
SodiuM phosphate Monobasic dihydrate
3-(PhenylaMinocarbonyl)-5-nitrophenylboronic acid
SalicylhydroxaMic acid, 99%
6'-CHLORO-2'-FLUORO-3'-METHYLACETOPHENONE
Methyl phenyl sulfone
2(2-Ethoxyethoxy)ethanol, pure, 98+%
2-Fluoropyridine-4-boronic acid
(S)-3-Amino-5-methyl-hexanoic acid
2,6-Dibromo-p-cresol
Magnesium hydroxide
Tetraethylammonium hydroxide
DULCITOL
3,4-Dimethoxybenzoic acid
Ethyl 3-(4-hydroxyphenyl)propanoate
2-BroMo-5-nitroanisole
4-Benzylphenol
3,3'-Bis(3,5-di-tert-butyl-4-hydroxyphenyl)-n,n'-hexaMethylenedipropionaMide
Hexyl chloroforMate, 98%
REINECKE SALT
2-BroMothiazole
2-amino-2-(furan-2-yl)ethanol
3,5-dimethyladamantane-1-carboxylic acid
3-Nitro-o-phenylenediamine
2,2-DIMETHYLTETRAHYDROPYRAN-4-ONE
N-PENTYLMAGNESIUM BROMIDE
Ethyl 5-(2,3-dichlorophenyl)oxazole-4-carboxylate
3-Acetyl-2,7-diMethyl-5H-[1]benzopyrano[2,3-b]pyridin-5-one
Anthracene-1,4,9,10-tetraol
Cyanoethyl sucrose
Glycyl-DL-alanine
Sodium Pentacyanoammineferroate(II) Hydrate
1-(4-amino-2-methylphenyl)-2-pyrrolidinone(SALTDATA: FREE)
Hexadecafluoroheptane
Phosphorus hepta sulfide
Oleuropein
Hexadecyltrimethylammonium hydroxide
N-(TriMethylsilyl)diethylaMine
1,3-Bis(4-hydroxybutyl)tetraMethyldisiloxane
2,6-Difluoropyridine-3-boronic acid hydrate
Cyclohexyl isocyanide, 99%
4-Chloro-3-nitrobenzoyl chloride, 98%
N-Acetyl-D-glucosaMine
4-(TrifluoroMethoxy)toluene
Diphenyl p-tolylphosphine
2,4-Difluoro-3-cyanophenylboronic acid
3-CHLORO-5-METHOXYPYRIDAZINE
Methyl 3-amino-4-phenylthiophene-2-carboxylate
TMSβ10 ELISA Kit
βACE1 ELISA Kit
Human lipoprotein lipase,LPL ELISA Kit
Human RPA-70 ELISA Kit
NA ELISA Kit
JAK3 ELISA Kit
AST ELISA Kit
Human vanillylmandelic acid,VMA ELISA Kit
Human Bromodeoxyuridine,BrdU ELISA Kit
VD ELISA Kit
AFPA Agar
Vitamin B12 Assay Broth
Indol-1-yl-Acetic Acid
BariuM ChroMate
5-AMino-3-(4-broMophenyl)-1H-pyrazole, 97%
2-Methyl-1-hexen-3-yne, 97%
2-Nitrobenzoic Acid
Calcium nitrate anhydrous
N,N-dicyclohexylmethylamine
tripelennamine hydrochloride

BOC-D-Leucine monohydrate
N-n-Butylbenzenesulfonamide
2-IMidazolidinone
2-BroMo-3,6-difluorophenylacetonitrile, 96%
Diethylene glycol diacrylate, tech. 75%, stab.
NeodyMiuM acetate
Picropodophyllotoxin
DIETHYLDITHIOCARBAMIC ACID FERRIC SALT
3-(N,N-DiMethylMyristylaMMonio)propanesulfonate
Ethylene-d4
(R,R)-(-)-2,3-DIMETHOXY-1,4-BIS(DIMETHYLAMINO)BUTANE
5-(4-Chlorophenyl)-2-thiophenecarbaldehyde
1-(tert-Butoxycarbonyl)-4-oxopyrrolidine-2-carboxylic acid, 97%
Trichloromethyl chloroformate
(2R,3S)-3-(N-BOC-amino)-1-oxirane-4-phenylbutane, 98%