BGC-823细胞：人胃腺癌复苏细胞系更多促销

BGC-823细胞：人胃腺癌复苏细胞系更多促销 货号：GD-C5718878

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-(TrifluoroMethyl)cyclohexanecarboxylic acid, cis + trans, 97%
EthylenediaMine Monohydrate
(1S)-(+)-MENTHYL ACETATE
BENZYL 6-HYDROXYNICOTINATE
2-Chloro-5-hydroxypyriMidine
TetraMethylaMMoniuM hydroxide
1-METHOXY-2-METHYL-2-PROPANOL
3-(Trifluoromethoxy)chlorobenzene
Bis(tricyclohexylphosphine)palladium (0), 98%
Hafnium(IV) t-butoxide (99.9%-Hf)
4-Boc-7-Fluoro-2,3,4,5-tetrahydro-1H-benzo[e][1,4]diazepine
Sulfenone
N-Methoxy-N-methylacetamide
1H-Pyrazole-1-carboxamidine hydrochloride
1-N-Boc-Aminocyclopentanecarboxylic acid
2,4-Dichlorofluorobenzene
3,5-DihydroxybenzaMide
N-Cyclohexyl 3-broMo-4-MethylbenzenesulfonaMide
TetraphenylphosphoniuM chloride, 98%
Octinoxate, USP
3,6-DiMethylphenanthrene
α-methylene-γ-butyrolactone
hex-5-en-1-yl acetate
2-Bromophenylacetylene
Chloroquine sulfate
4-Acetamidophenylboronic acid
4-Butylbenzoic acid
4-Chloro-6-Fluoroquinoline
(S)-(-)-3,3'-DibroMo-1,1'-bi-2-naphthol, 96%
2,2,3,3,4,4,5,5-Octafluoropentyl acrylate, 97%, stab. with ca 50ppM 4-Methoxyphenol
3,4-(Methylenedioxy)benzeneboronic acid, 98%
Quinoline
Dodecyl aldehyde
SELENIUM CHLORIDE
1,4-Didodecyl-2,5-diiodobenzene
N-AMINOPHTHALIMIDE
Isobutyl 2-butenoate
Sodium 2,4-Dimethylbenzenesulfonate Monohydrate
1-(1-Aminoethyl)adamantane hydrochloride
Lacto-N-neotetraose ,>=95%
(3-oxopiperazin-1-yl)acetic acid(SALTDATA: FREE)
4-Methoxybenzylchloride
2-METHYL-5-NITRO-1H-INDOLE-3-CARBALDEHYDE
2-Methylbenzotrifluoride
Pyridine hydrofluoride
4-AMino-1,2,5-oxadiazole-3-carboxylic acid
1,4-Dipropylbenzene
N-Butyl 4-broMobenzenesulfonaMide
Tetrahydro-2H-pyran-2-ol, 90%, cyclized forM of 5-Hydroxypentanal
NeostigMine Methyl Sulfate
Valeroin
Isobutyl chloroformate
1-(2-Pyridine)Benzylcyamide
4-oxobutanoic acid
Palmoxiric acid
Diethyl succinate
4-Chloro-3-(isopropylcarbaMoyl)phenylboronic acid
N-Cyclopropyl 3-broMo-5-nitrobenzaMide
2-AMino-7-isopropyl-5-oxo-5H-[1]benzopyrano[2,3-b]pyridine-3-carbonitrile
DI-U-CHLOROBIS(5-HYDROXY-2-(1-(HYDROXYI&
4,4'-Difluorobenzhydryl chloride 98%
3-(3-m-Tolyl-ureido)-propionic acid
1-(4-Methoxyphenyl)ethanamine
(4-bromophenoxy)acetonitrile(SALTDATA: FREE)
3-Bromocumene
4-Bromobenzyl chloride
N-Acetyl-L-leucine
2-(PhenoxyMethyl)benzylaMine
1,3,5-Trifluorobenzene
(3,4-DiMethoxybenzyl)triphenylphosphoniuM BroMide
4-BroMo-3-Methylthiophene-2-carbaldehyde
4-Fluorobenzyl chloride, 98%
Thiabendazole, USP
TetraMethylurea
2-Difluoromethyl-1H-benzoimidazole
2-Amino-3-chloropyrazine
Molybdenum disulfide
Hexahydropyridazin
AT2R1-Ab ELISA Kit
Mouse interferon-inducible protein 16,IFI16/p16 ELISA Kit
Human FABP ELISA Kit
Human CAPN2 ELISA Kit
ADM ELISA Kit
Rat PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit
IL-8/CXCL8 ELISA Kit
Human FRS2 (Fibroblast Growth Factor Receptor Substrate 2) ELISA Kit
Human TP5 (Thymopentin) ELISA Kit
DEHA-S ELISA Kit
Potato Dextrose Water
Martin Agar
Indole-4-boronic acid, 95%
Heptaldehyde
5-AMinoquinoline, 99%
2-Methyl-2-nitro-1,3-propanediol, 97%
α-methylene-γ-butyrolactone
Chromium(III) sulfate hydrate
N-acetyl-D-(+)-glucosamine
Hydroquinone Dimethyl

BENZENESULFINIC ACID SODIUM SALT DIHYDRATE
2,7-Naphthalenedisulfonic acid disodium salt
Ethyl 5-BroMonicotinate
2-Cyano-4-Methylpyridine, 98%
Methyl 6-(hydroxyMethyl)nicotinate, 98%
3-Pentanol
Auric oxide
Triethyl phosphite
NITROBENZENE-D5
2,3,5,6-TETRAFLUORO-4-HYDRAZINOBENZOTRIFLUORIDE
2-MethylcyclohexylaMine
3-Bromo-2-chlorothiophene, 97%
N,N,N'-Triethylethane-1,2-diamine
Dibenzothiophene-4-boronic acid
Scopolamine hydrobromide trihydrate, 99%