bEnd.3[BEND3]细胞：小鼠脑内皮瘤复苏细胞系更多促销

bEnd.3[BEND3]细胞：小鼠脑内皮瘤复苏细胞系更多促销 货号：GD-C5718889

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Cyclohexyl p-toluenesulfonate, 97%
3-Mercaptopropionic Acid Isopropyl Ester
DESYL CHLORIDE
Diethylene Glycol Monovinyl Ether (stabilized with KOH), 96%
2-MethylbenzaMide
3,4-Methylenedioxyacetophenone
2,2,3,4,4,4-HEXAFLUORO-1-BUTANOL
Purine, 99%
5'-Fluoro-2'-methoxyacetophenone
Methyl Hydroxyethyl Cellulose (20-40mPa.s, 2% in Water at 20deg C)
4-(Chloromethyl)-1H-imidazole hydrochloride
DANSYL-L-PHENYLALANINE
Tetrahydrofurfuryl chloride
1-(4-METHOXYPHENYL)-1H-PYRROLE
Tosylmethyl isocyanide
5-Nitrothiophene-3-carboxylic acid
(4R,5R)-1,3-DiMethyl-4,5-diphenyl-2-[(S)-1-benzyl-2-hydroxyethyliMino]iMidazolidine
N-Cyclopropyl 2-broMobenzaMide
ThioacetaMide, 99+%, ACS reagent
o-PhenylenediaMine, Reagent
2-AMinophenol Hydrochloride
drofenine hydrochloride
2-Methylpyrimidine
3-iodoanisole
2,4-Dichlorobenzoyl peroxide
3-CHLOROBENZYLIDENEACETONE
Bismuth trichloride
Acid Red 27
Bis(2,2,6,6-tetraMethyl-3,5-heptanedionato)MagnesiuM(II) hydrate, 98+%
Methyl 2-Methylnicotinate, 97%
Stearyl Methacrylate
Methyl 3-thiophenecarboxylate
N-Methoxy 3-borono-4-fluorobenzaMide
ScandiuM(III) chloride hexahydrate
2-Methoxyisonicotinohydrazide
N,N'-Bis(2-aMinoethyl)-1,3-propanediaMine
2-(4-Fluoro-phenyl)-4-methyl-thiazole-5-carboxylic acid
Ethyl 3-[(6-methylpyrazin-2-yl)oxy]benzoate , 97%
N,N'-Diacetylethylenediamine
Ethyl 3-amino-4-cyano-5-(methylthio)-thiophene-2-carboxylate
1-NAPHTHALENEACETIC ACID
3-Mercapto-1,2-propanediol
Vagistat
Cupric acetate
Perchloric acid
N-(3-AMinopropyl)glycine Dihydrochloride
N-Benzyl-4-(benzylaMino)picolinaMide
3-Methylpyrazole, 99%
N-BOC-p-phenylenediaMine, 97%
4-NITROPHENYL-ALPHA-D-GLUCOPYRANOSIDE
4-Hexylaniline
Ethylene glycol
Fmoc-DL-serine
(1R,2R)-1,2-Cyclohexanedimethanol
2-Amino-5-bromobenzonitrile
2,4-Dibromophenol
TetraMethylguanidine
6,6-DIMETHYLBICYCLO(3.1.1)HEPT-2-ENE-2-ETHANOL
3-NORADAMANTANECARBOXYLIC ACID
Phenyl trifluoroacetate 98%
Diphenylamine Sulfate
4-Fluoro-2-methoxybenzyl alcohol
5-Methyl-1-phenylpyrazole-3-carboxylic acid
2-Deoxy-D-glucose
Amrinone
Homovanillic acid
3-Thiopheneboronic acid
(S)-(-)-alpha-Methylbenzyl isocyanate
1,3-Benzodioxole-5-carbothioaMide
4'-Hexyloxybenzylidene-4-cyanoaniline
Boc-3-aMinoMethyl-phenylacetic acid
3-Nitroanisole, 99%
TRILAURIN
N-(tert-Butoxycarbonyl)-4-chloro-L-phenylalanine
1,12-diaminododecane
Silicon dioxide
2-Nitrophenylacetic acid
Lionolblue GS
AT-2 ELISA Kit
γABRα2 ELISA Kit
Lipase ELISA Kit
Human histatin 5,HTN5 ELISA Kit
T3 ELISA Kit
COL1 ELISA Kit
GSTs ELISA Kit
Human very late appearing antigen 4,VLA4 ELISA Kit
Human androgen receptor,AR ELISA Kit
SIGLEC12 ELISA Kit
Czapek Dox Agar
Bean Agar Medium
Methyl indole-3-carboxylate, 99%
Mordant Blue 29
5-AMinopyridine-2-carboxylic acid, 98%
2-Methyl-2-Thiopseudourea HeMisulfate
drofenine hydrochloride
Sodium Molybdate Dihydrate
4-Methylmorpholine-N-oxide
Verapamil hydrochloride

TRIS(6,6,7,7,8,8,8-HEPTAFLUORO-2,2-DIMETHYL-3,5-OCTANEDIONATO)YTTERBIUM
Cbz-L-tert-Leucine
3,4,5-TribroMopyrazole
2-BroMoiMidazole, 95%
4-Fluoropiperidine hydrochloride, 95%
Lys-Lys-Lys
1-BroMo-3-chlorobenzene
2-Methoxythiophene
Nitroethane
L-(+)-Fructose , 97%
1-Cyclohexyl-1-pentanol, 98%
trans-4-Hydroxystilbene, 98%
(E)-(Hexen-1-yl)boronic acid 98%
4'-Fluoro-[1,1'-biphenyl]-4-carboxaldehyde
Nitrosobenzene