LLC-MK2细胞：恒河猴肾复苏细胞系技术强

LLC-MK2细胞：恒河猴肾复苏细胞系技术强 货号：GD-C5319188

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Acetophenone, 99%
L-Proline benzyl ester hydrochloride
Pyridine-2-boronic acid
2,3,5,6-TETRABROMO-4-METHYLPHENOL
POTASSIUM TRI-SEC-BUTYLBOROHYDRIDE
1-(4-BroMo-3-trifluoroMethylphenyl)pyrrolidine
NORBORNANE
6-Amino-5-bromo-3-pyridine sulfonic acid
1,2,3-Propanetricarboxylic Acid
4'-Carboxybenzo-18-crown 6-Ether
2-(4-Methoxyphenyl) methyl phenol
CLEMASTINE FUMARATE
2-Bromo-9-fluorenone
5-Nitro-1,10-phenanthroline
2-Methyl-1,3-thiazole-4-carboxylic acid
3-AMino-4-Methylpyridine
DiMethoxydi-p-tolylsilane
XyloMetazoline Hydrochloride
Uridine, 99%
SodiuM Nitrite, Granular, USP
4-ChlorobenzylaMine
homatropine hydrochloride
hexyl chloroformate
4-mercaptobenzoic acid
2-Fluoro-5-nitroanisole
1-Benzyl-4-hydroxypiperidine
Carrot Seed Oil
Undecylenic Acid
Bis(cyclopentadienyl)vanadiuM, subliMed, 95%
Nitrobenzene-d{5}, 99% (Isotopic)
(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt
Nα-Benzoyl-DL-arginine β-naphthylaMide hydrochloride
(R)-(+)-Trityl glycidyl ether
PENTAMETHYLENEBIS(TRIPHENYLPHOSPHONIUM BROMIDE)
3-(TriMethylsilyl)propynoic acid
4-(2-CHLOROETHYL)BENZOIC ACID
6-(2-Hydroxyethylamino)-1,3-dimethyl-1H-pyrimidine-2,4-dione ,98%
N,N-Dipropylaniline
4-Chloro-3,5-difluorobromobenzene 97%
(S)-(-)-2,2'-Bis(di-p-tolylphosphino)-6,6'-dimethoxy-1,1'-biphenyl, min. 97%
Isobutylnitrite
trans-4-Pentylcyclohexanecarboxylic acid
p-Anisidine
1-Chloropinacolone
4-Nitro-o-xylene
AluMinon
N-Ethyl-N-(2-hydroxyethyl)nitrosaMine
N-Acetyl 2-broMo-5-chloroaniline
2-AMino-3-broMo-5-Methylpyridine, 98%
DIPROPYLENE GLYCOL
trans-3-BroMocinnaMic Acid
1H-Purin-6-amine, 2-methyl-
Hyaluronidase
4-Dimethylaminostyrene
2-Cyanoacetanilide
4,6-Dihydroxy-2-methylpyrimidine
TriMethyl cis,cis-1,3,5-cyclohexanetricarboxylate
Tetranitroblue tetrazoliuM chloride
RhodiuM carbonyl chloride
(S)-3-(1-Aminoethyl)anisole
Glycyl-DL-valine
1-methyl-4-phenyl-1H-pyrazol-5-amine(SALTDATA: FREE)
Ruthenium(III) iodide, anhydrous, 98+%
o-Anisic acid
1,2-Dodecanediol
3,5-DIFLUOROBENZOPHENONE
Diethylaminosulfur trifluoride
Benzaldehyde
1-Benzyl-5-phenylbarbituric Acid
3-CyanoMethoxyphenylboronic acid, pinacol ester
Chloroxazone, 98%
Methyl 4-(chloroMethyl)benzoate, 97%
3-Methyl-2-pentene (cis- and trans- Mixture)
7-Methoxy-4-quinolinol
Cyclohexyl isocyanide
4,5,6,7-tetrahydro-5-methyl-Thiazolo[5,4-c]pyridine-2-carboxylic acid hydrochloride
a-D-Glucopyranoside,methyl 4-O-[2-O-acetyl-6-methyl-3-O-(phenylmethyl)-a-L-idopyranuronosyl]-2-deoxy-2-[[(phenylmethoxy)carbonyl]amino]-3-O-(phenylmethyl)-,6-acetate
dl-thiorphan
CA125 ELISA Kit
TAF2 ELISA Kit
Human TTR ELISA Kit
Human USP8 ELISA Kit
Chicken Interleukin 18,IL-18 ELISA Kit
AFU ELISA Kit
NF-κB ELISA Kit
Human listeriolysin O,LLO ELISA Kit
Human HBα1 ELISA Kit
FUCa1 ELISA Kit
Eugon Agar
StandaredⅠNutrient Agar
Ethoxyacetic acid, 98%
AMMoniuM Tetrafluoroborate
5,7-DibroMo-8-hydroxyquinoline, 98%
2-Fluoro-5-(hydrazinocarbonyl)benzeneboronic acid, 95%
homatropine hydrochloride
2-CHLOROISOBUTYRYL CHLORIDE
octacarbonyldicobalt
3-Fluorocinnamic acid

(R)-(-)-4-Phenyl-2-oxazolidinone
POLY[1,6-HEXANEDIOL/NEOPENTYL GLYCOL/DI(ETHYLENE GLYCOL)-ALT-ADIPIC ACID] DIOL
(S)-2-AMino-4-Phenylbutyric Acid
1,1,3,3-Tetraethylurea, 99%
3-(Methylsulfonyl)benzeneboronic acid, 98%
2,5-Dichloro-N-(2-fluorobenzyl)aniline, 97%
BroMochloroMethane
5-Fluoro-1,3-isobenzofurandione
Perfluoro(allylbenzene), 97%
ACETONE-D6
1-Iodonaphthalene
3-Fluoro-4-Methoxybenzaldehyde
3-Bromo-4-chlorotoluene
Stearyl Methacrylate (stabilized with MEHQ)
1H-Pyrrolo[2,3-b]pyridine-4-carboxylic acid