MEF-11细胞:小鼠胚胎成纤维复苏快细胞系 货号:GD-C4720181
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Methyl acrylate, 99%, stab. with ca 15ppM 4-Methoxyphenol
Dibutyl Adipate
3-BroMo-2-isopropoxy-5-forMylphenylboronic acid
N-PHENYL-THIOBENZAMIDE
Methyl 6-fluorochroMone-2-carboxylate
TRIBUTYLPHENYLTIN
4-Fluoro-2-MethoxybenzylaMine, 97%
3-Hydroxypropionic Acid (contains varying amounts of 3,3'-Oxydipropionic Acid) (ca. 30% in Water, ca. 3.6mol/L)
Ethyl 4,4-difluoro-2-(ethoxymethylene)-3-oxobutanoate
1-(3-Bromo-4-methoxyphenyl)ethanone
(4S)-4-Benzyl-L-glutamic acid
DL-NORADRENALINE HYDROCHLORIDE
AZOXYBENZENE
3-AMINO-2,6-DIMETHYLPYRIDINE
5-Chloro-2-nitro-4-(trifluoroMethyl)aniline
4,6-DiMethyl-2-MercaptopyriMidine
Diethyl (4-Isopropylbenzyl)phosphonate
N-Ethyl 2-broMobenzaMide
Trichloroacetyl chloride, 97%
PotassiuM Carbonate, Anhydrous, Granular, USP
Diethyl 3-Hydroxyglutarate
2,3-Dibromosuccinic acid
3,4-Diethoxybenzoic acid
1-Methyl-2-pyrrolidinone
2,5-Dinitrophenol
3-Fluoro-2-hydroxybenzaldehyde
4-Methylbenzyl alcohol
1-Ethyl-2,3-DiMethyliMidazoliuM Chloride
2-BroMo-4,6-difluoroanisole, 97%
5-Nitrothiophene-2-carboxylic acid, 98%
OxaMyl
1,2,4-Tributylphosphorotrithioate
2-AMino-6-fluorobenzothiazole
PRASEODYMIUM BROMIDE
PENTYL ISOTHIOCYANATE
DICHLOROMETHYLOCTYLSILANE
Ethyl 2-chlorooxazole-4-carboxylate
7-Fluoroisatin
1,2,4,5-Tetrabromobenzene
Boc-(R)-3-amino-4-(2-thienyl)-butyric acid
1-(3-Pyridyl)-3-(dimethylamino)-2-propen-1-one
2-FLUOROBENZOTRICHLORIDE
Deoxycholic acid
3-AMINO-5-MORPHOLINOMETHYL-2-OXAZOLIDINONE
4-BroMo-2-fluorobenzylaMine
Triacetoxy(Methyl)silane
2',3',5'-Tri-O-acetylguanosine
2-Naphthoyl chloride
8-Methylquinoline, 97%
2-Iodopropane
Tetrakis(triphenylacetato)dirhodiuM(II) DichloroMethane Adduct
isonicotinic acid
3,5-DIMETHYLMORPHOLINE-4-CARBOXAMIDINEHEMISULFATESALT
2-(phenylcarbonyl)-1,2,3,6,7,11b-hexahydro-4H-pyrazino[2,1-a]isoquinolin-4-one
4-(3-thienyl)benzoic acid
3-(4-ETHYLBENZOYL)PROPIONIC ACID
Acid Yellow 23
COBALT IRON OXIDE
4-(MethylaMino)benzoic acid
4-Biphenylmagnesium bromide, 0.5M solution in THF, AcroSeal
4-Methoxyphenylhydrazine hydrochloride, 98%
4-Chloro-3-(trifluoromethyl)phenylboronic acid pinacol ester
2-(2,6-Dichloro-benzylsulfanyl)-ethylamine
3-Iodobenzaldehyde
DIETHYL IODOMETHYLPHOSPHONATE
Bromodiphenylmethane
Scandium oxide
Pyrrolidine-1-carboxiMidaMide hydroiodide
1,4-Dichlorophthalazine
SodiuM 1-Naphthol-4-sulfonate
FMoc-Lys(FMoc)-OH
AdaMantane-1-carbonitrile, tech
2-Methoxythiophene
2-AMinobenzophenone
3,3,4,4,5,5,6,6,6-nonafluorohexene
Methyl 3-oxo-2-(pent-2-enyl)cyclopentaneacetate
Bis(triphenylphosphine)nickel(II)chloride
Acid Red 35
CCND1 ELISA Kit
Mouse β2-glycoprotein 1 antibody IgA/G/M,β2-GP1 IgA/G/M ELISA Kit
Human TNS3 ELISA Kit
CD109 ELISA Kit
Chicken Myoglobin,MYO/MB ELISA Kit
Rat GAP (Ras Gtpase Activating Protein) ELISA Kit
Chicken IL-13 (Interleukin 13) ELISA Kit
CRH ELISA Kit
ACE2 ELISA Kit
ALPI ELISA Kit
TYA Medium Base
Potato dextrose agar
METHYL ISOCYANOACETATE
BARIUM PERCHLORATE TRIHYDRATE
5-(ChloroMethyl)-3-Isopropyl-1,2,4-Oxadiazole
2-Fluoropyridine
2,3-Dibromosuccinic acid
5-Methoxyindole-3-carboxaldehyde
Potassium fluoride
1-Phenyl-2,2,2-trifluoroethanol
2,4,6-TRIFLUOROPYRIMIDINE
BIS(DIBUTYLCHLOROTIN) OXIDE
FMoc-D-Pro-OH
CinnaMaldoxiMe, (E)+(Z), 98%
1H-Indazole-3-carboxylic acid, 98%
3-Indoleacetic acid
CalciuM peroxide
trans-1,2-Bis(phenylsulfonyl)ethylene , 98%
5,5′,6,6′,7,7′,8,8′-Octahydro-1,1′-bi-2-naphthol
2,5-Furandicarboxylic acid
4-METHOXY-2-NITROBENZONITRILE
1-Methylhydantoin 97%
Pyrido[3,2-d][1,3]thiazole-2-thiol, 97%
1-hydroxycyclopentanecarboxylic acid(SALTDATA: FREE)
Dipropyl Sulfate [Alkylating Agent]