MC3T3-L1细胞：小鼠胚胎成纤维复苏快细胞系

MC3T3-L1细胞：小鼠胚胎成纤维复苏快细胞系 货号：GD-C4720178
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞，T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞，可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞，并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Chloro-N-MethylacetaMide, 97%
HexachloroiridiuM Acid Hydrate
3-BroMo-2-broMoMethyl-1-propene
Acetosyringone
ETHYL 4-CHLORO-6,8-DIMETHYLQUINOLINE-3-CARBOXYLATE
4-AMINO-N,N-DIMETHYLBENZAMIDE
Bis[(triMethylsilyl)tributyl]stannyl phosphate
(4-bromo-3,5-dimethyl-1H-pyrazol-1-yl)acetonitrile(SALTDATA: FREE)
Vinyldiphenylphosphine, min. 97%
2-Methylbenzyl isothiocyanate
4-Cyano-2-iodopyridine 98%
MILTEFOSINE
Bis(1,5-cyclooctadiene)rhodium(I) tetrafluoroborate
N-BENZYL-L-PROLINE
EDCI
TributylaMine
1,3-DibroMo-5-(trifluoroMethoxy)benzene
N-(2,6-DiMethylphenyl) 2-broMobenzaMide
Trichloroacetic acid, 99%, extra pure
PoloxaMer 407, NF
Mordant Blue 13
2,6-Dibromoaniline
L-Lysine L-glutamate
2-Mercaptoimidazole
Methyl 4-fluoro-3-nitrobenzoate
3,5-Dibenzyloxyacetophenone
NYLON 6/66
1,3,3-TriMethyl-2-Methyleneindoline
SodiuM tetrafluoroborate, 97%
Quinoline-4-carboxylic acid, 98+%
SodiuM oxalate
Phenylhydrazine hydrochloride
O-BENZYL-L-SERINE
PRASEODYMIUM(III) IODIDE
2,6-Dichloro-3-Methylpyridine
1-(2-Chlorophenyl)
4,5-Dibromo-1H-imidazole 98%
d-Leucinol
Disodium beta-Glycerophosphate Tetrahydrate [for Biochemical Research]
3-[(tert-Butoxycarbonyl)amino]-3-(4-methylphenyl)propanoic acid
N,N'-(3,3'-Dimethyl-4,4'-biphenyldiyl)bis(3-oxobutanamide)
4-ETHOXYSTYRENE
5-Phenylhydantoin
5-Methylphenazinium methosulfate
Cobalt tetraMethoxyphenylporphyrin
5-AMino-1-naphthalenesulfonic acid
trans-4-(HydroxyMethyl)cyclohexanecarboxylic Acid
2-Chloro-4-hydroxy-5-fluoropyriMidine
BOC-trans-4-hydroxy-L-proline, 98%
6-PHOSPHOGLUCONIC ACID TRISODIUM SALT
4-Nitrobenzyl Alcohol
4,4'-cyclohexane-1,1-diylbis(2-cyclohexylphenol)
Vinyltriisopropoxysilane
2,3-Dimethoxyphenol
2-allyl-1,4-bis[[3-(3,4-dihydroxyphenyl)-1-oxo]oxy]-3,5-dihydroxycyclohexanecarboxylic acid
(-)-ANABASINE
2-Isopropyl-4-Methylthiazole-5-boronic acid, pinacol ester
2-BroMo-5-chlorophenylboronic acid
CalciuM dipropionate
Glycyl-4-aminobutyric Acid
n-Propyldichlorophosphine, min. 98%
Triphenylantimony Diacetate
1,4-Cyclohexanedimethanol Diisovalerate
N-ACETYLPHTHALIMIDE
(S)-N-FMOC-AZETIDINE-2-CARBOXYLIC ACID
Thifensulfuron methyl
Tungsten
[4-(1,3-Thiazol-2-yl)phenyl]MethylaMine
1,4-DibroMonaphthalene
Methyl trans-4-Hydroxycyclohexanecarboxylate
5-Fluoro-2-Methyl-4-nitroaniline
BroMoforM, 96%, stabilized
MYRISTOLEIC ACID
Chlorogenic Acid Hydrate
DYNASYLAN 9407
3-Pyridinecarboxaldehyde, 2-Fluoro-
Ehoxyphenyl; 97%
3,7-Diazabicyclo[3.3.0]octane
FLNa ELISA Kit
β2-GP1 Ab lgA ELISA Kit
Human LATS ELISA Kit
Human SNX10 ELISA Kit
MYO/MB ELISA Kit
SP-Selectin/GMP-140/CD62P ELISA Kit
Chicken IL-12 p40 (Interleukin 12 p40) ELISA Kit
Human FSH ELISA Kit
Human Angiotensin converting enzyme,ACE ELISA Kit
ADA ELISA Kit
Clostridia Count Agar
Yeast Peptone Dextrose Adenine Medium
Methyl isocyanoacetate, 95%
DysprosiuM(III) perchlorate hexahydrate, 50% w/w aq. soln., Reagent Grade
5-Chloroquinaldine
2-Fluoropyridine-3-boronic acid pinacol ester, 95%
2,6-Dibromoaniline
1-(5-methyl-2-furyl)propan-1-one
Copper(II) fluoride
9a-fluoroprednisolone

2-Indanylacetic acid
n-Undecane
RhodiuM(III) sulfate, bright plating solution, Rh 5% (cont. Rh)
1-Boc-3-iodoazetidine, 97%
Iron powder, reduced, 99%
PhenylMethanesulfonyl fluoride
RhodiuM acetate
Boc-Phe-ONp
4-(TRIFLUOROMETHYL)PHENYL ISOCYANATE
NeopentylaMine
3-ETHOXYBENZHYDRAZIDE
Manganese(IV) oxide, powder, 80-85%
5-Phenyl-1,3-oxazole, 95%
Ethyl pentafluoropropionylacetate
Bis(tricyclohexylphosphine)[(phenylthio)methylene]ruthenium(II) dichloride, min. 97%