HT-144细胞：人恶性黑色素瘤复苏快细胞系 货号：GD-C4720163
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞，T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞，可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞，并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2'-Deoxyguanosine hydrate, 99%
Methyl stearate
3-BroMo-5-butoxyphenylboronic acid
ETHYL 2,5-DICHLOROBENZOATE
2,4,5-Trifluorophenyl isocyanate
Mercaptosuccinic acid
2-Chloro-4,6-bis[3-(perfluorohexyl)propyloxy]-1,3,5-triazine
Propiolic Acid
1-Pentyn-3-ol
3,6-Dibromo-o-xylene
t-Butyl dimethyl silanol
6,7-Dihydro-5-methyl-5(H)-cyclopentapyrazine
2,3,4-Trihydroxybenzoic acid
gamma-Butyrolactone
1-Methylpiperidine
Piperonyl alcohol
1,1'-Dipropylhafnocene Dichloride
1,4-diMethyl 2-broMobenzene-1,4-dicarboxylate
Triacontane, 98%
PolyMyxin B Sulfate, USP
Propionyl BroMide
2,4-dichloroquinazoline
2-Bromo-1-indanol
n-Heptyltrichlorosilane
DL-Leucine
2-PIPERIDYLMETHYLAMINE
(TCNQ)2 ISOQUINOLINE(N-N-BUTYL)
4-BroMo-1-butene
2-AMino-3,6-difluorobenzonitrile, 97%
3-Carboxybenzaldehyde, 97%
AMMoniuM iron(III) citrate
N-Acetyl-L-tryptophan ethyl estert
PHENYLTHIOHYDANTOIN-ASPARTIC ACID
Propyl disulfide
SodiuM triMethylacetate hydrate
N-CyanoiMido-S,S-diMethyl-dithiocarbonate
4-Methoxy-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical [Catalyst for Oxidation]
(2S,4R)-1-tert-Butyl 2-methyl 4-hydroxypyrrolidine-1,2-dicarboxylate
4-Bromo-2-methylaniline
Potassium L-Aspartate Hydrate
2,6-Difluorobenzyl chloride
Boc-3-(2-Naphthyl)-D-alanine
2,6-Di-tert-butyl-p-benzoquinone
3-METHYLADENINE
4-Propoxyphenylboronic acid (contains varying aMounts of Anhydride)
(R)-N-Boc-Propargylglycine
p-Tolualdehyde 2,4-Dinitrophenylhydrazone
2,6-Dichlorotoluene, 99+%
Chrysophanic acid, 98%
Loratadine
N-(4-BroMophenyl)phthaliMide
3-(2H-benzotriazol-2-yl)-4-hydroxy phenethyl alco
Cypermethrin, High Effect
P-phenylbenzaldoxime
3-Methoxybenzoylacetonitrile
BARIUM IODIDE
3-Methyl-4-nitrobenzonitrile, 97%
Phenylhydrazine
TIN(II) OXIDE
6-Fluoroindole-3-acetonitrile
dl-Mandelamide
4-Bromo-2-fluoro-N-methylbenzamide
Quinoline-8-sulfonic Acid Sodium Salt
3-ACETOXY-2-METHYLBENZOYL CHLORIDE
4,5-Dibromothiophene-2-sulphonyl chloride
3-Formyl-6-isopropylchromone
VinyltriMethoxysilane;(TriMethoxysilyl)ethylene;Y-4302
2,6-Dichlorophenylhydrazine hydrochloride
1,4-Butanediol DiMethanesulfonate
4-BroMoMethyl-2-chlorophenylboronic acid, pinacol ester
8-Anilino-1-naphthalenesulfonic acid aMMoniuM salt hydrate, 97%
N1-(4-AMinophenyl)-N1-MethylacetaMide, 97%
3-Hexylthiophene
3-(TriMethylsilylethynyl)pyridine
N-(N-glycylglycyl)glycine
Entecavir hydrate
1-Bromo-3-methyl-5-(trifluoromethyl)benzene
1,4-Bis((2,6-diethyl-4-methylphenyl)amino)-9,10-anthracenedione
PAI 1 ELISA Kit
Mouse β-galactosidase,βGAL ELISA Kit
Human CENP-B ELISA Kit
Human SNX8 ELISA Kit
αFP ELISA Kit
S.P ELISA Kit
Chicken Interferon γ ,IFN-γ ELISA Kit
Human TSH ELISA Kit
Human ANG II R1/AGTR1 (Angiotensin II Receptor 1) ELISA Kit
FRA ELISA Kit
Trypticase peptone Glucose Yeast Extract Beef
HC Agar Base
Isoquinoline-5-sulfonic acid, 95%
LithiuM Perchlorate Trihydrate
5-Chloro-2-Hydroxybenzaldehyde
2'-Fluorobiphenyl-4-carboxylic acid, 97%
2,4-dichloroquinazoline
3-(p-Nitrophenoxy)propionic Acid
1-Chloroethyltrichlorosilane
1,4-dibromooctafluorobutane
CHLOROFORM-D
5-Chloro-2-methoxyphenylboronic acid
METHYL 2,3-DIFLUOROBENZOATE
Glycidyl 4-Methoxyphenyl ether, 98%
Hexafluoroglutaryl chloride, 97%
(R)-1-FMoc-piperidine-3-carboxylic acid
1,1-Dichloropropene Standard
PYRIDINE-3,4-DICARBOXYLIC ANHYDRIDE
2,2,3-TRIBROMOBUTANE
s-Trioxane
5-Hydroxy-1-indanone
3-Amino-4-methylbenzotrifluoride 98%
Methyl 4-aminothieno[2,3-d]pyrimidine-6-carboxylate
Boc-N-Me-Val-OH dicyclohexylamine salt
trans-2-Decen-1-ol
