GAK细胞：人恶性黑色素瘤复苏快细胞系 货号：GD-C4720145
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞，T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞，可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞，并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1,2,4,5-TetraMethylbenzene, 97+%
Benalaxyl
2-PhthaliMidoMethylphenylboronic acid, pinacol ester
M-CYMENE
2-Hydroxypyridine
3-Chloro-5-(trifluoroMethyl)phenylboronic acid
(-)-MENTHYL CHLORIDE
4-Methoxy-3,5-dimethyl-2-pyridinemethanol ,98%
Ethyl propionylacetate, 98+%
3,4,6-Tri-O-acetyl-2-deoxy-2-phthalimido-α-D-glucopyranosyl fluoride
1-(2-Fluoro-benzyl)-
Pentabromophenol
CESIUM TRIFLUOROACETATE
Ammonium iron(III) sulfate
6-Chloro-1-hexyne
5-Methoxyindole-3-acetic acid
2,4-DiaMino-6-[2-(2-undecyl-1-iMidazolyl)ethyl]-1,3,5-triazine
N,N-Diethyl 5-broMo-2-MethoxybenzenesulfonaMide
trans-beta-Methylstyrene, 97%, stabilized
PiracetaM, EP
4-Cyclohexylcyclohexanol
Metanil Yellow
Benzyl acetoacetate
L-Cysteine ethyl ester hydrochloride
trans-1,2-Dichloroethylene
4-Bromophenylboronic acid
Titanium dioxide
2-AMino-3,4-DiMethylpyridine
SodiuM hexachlororhodate(III) dodecahydrate, Rh 17.1%
3-BroMoquinoline, 98%
Butyl lactate
3-Quinolineboronic acid pinacol ester
2-BroMo-1,4-dichlorobenzene
P-XYLENOL BLUE
1S,2S-1,2-Di(4'-Methoxyphenyl)-1,2-diaMinoethan
(S)-(+)-1,2,3,4-Tetrahydro-1-naphthol
Digitonin
2',5'-Dichloroacetophenone
2-(3-Methoxyphenyl)thioacetamide
Bis(3-nitrophenyl) Disulfide
2,6-DICHLORO-4-(TRIFLUOROMETHYL)BENZAMIDE
1,3,5-TRIMETHOXY-2-NITROBENZENE
Dibutyltin dilaurate
BARIUM TRIFLUOROMETHANESULFONATE
trans-4-Hydroxy-L-proline Methyl ester hydrochloride
Cyanoacetylurea
3,3'-Methylenediindole
3,5-DiMethylpyridin-4-aMine
1,3-Thiazol-2-ylMethylaMine, 97%
2-BENZYL-2-IMIDAZOLINE HYDROCHLORIDE
2-Chloro-4-fluorobenzoyl Chloride
N-Benzyl-1,3-propanediamine
Mecillinam
Demethyl
3-(3-Hydroxyphenyl)-1,1-dimethylurea
4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl morpholinium chloride
2-Hydroxy-4-Methoxybiphenyl-3-carboxylic acid
1-(2-METHYLPHENOXY)-2-PROPANOL
N-Methylindole-2-tributylstannane
3-(Trimethylsilyl) propiolic acid
2-Acetyl-3-ethylpyrazine
7-Chloroquinoline-4-boronic acid, pinacol ester 90%
Bis (triethoxysilyl propyl) disulfide
[5-[[4-[2-(5-Ethyl-2-pyridinyl)ethoxy]phenyl]methyl]-2,4-] thiazolidinedione hydrochloride
THALLIUM(I) IODIDE
3,5-Dichlorobenzonitrile
CI 13015
Tri-tert-butylphosphoniuM tetrafluoroborate
1,4-Bis(5-phenyl-2-oxazolyl)benzene
4-(Methylthio)-M-cresol
6-Methylpyrazine-2-carboxylic acid
ChroMone-3-carboxaldehyde, 98%
METHYL 2,5-DIHYDROXYCINNAMATE
ThioMichler's Ketone [Sensitive spectrophotoMetric reagent for Au, Ag, Hg and Pd, use for the deterMination of residual chlorine]
CASSIA OIL
Sodium 4-trifluoromethylbenzoate
Cis-Hexahydrophthalimide
4-Methyl-2-phenylpyridine
FSP ELISA Kit
Aβ42 ELISA Kit
Human eEF2 ELISA Kit
Human SFRP1 ELISA Kit
CTGF ELISA Kit
P53 ELISA Kit
Chicken α-Glu (Alpah Glucosidase) ELISA Kit
Human EPO ELISA Kit
Human AT2R ELISA Kit
Guinea pig plasmin-antiplasmin complex,PAP ELISA Kit
Fluid Thioglycollate Medium
Yeast Mold Broth
ISOSORBIDE DIMETHYL ETHER
TETRAETHYLAMMONIUM PERCHLORATE
5-Chloroindole, 98%
2'-Fluoroacetanilide, 98+%
Metanil Yellow
sulfur dichloride
Magnesium chloride
1-(2-chloroethyl)-4-fluorobenzene
2,4-Di-tert-pentylphenol
SODIUM BIFLUORIDE
1,2-Dibromotetrafluoroethane
Silicon(IV) iodide, 99% (Metals basis)
Phenyl trifluoroMethyl sulfide, 98%
2-Fluorothiophenol
Penbutolol solution
2-Methyl-5-nitrobenzenesulfonic acid
8-PHENYL-1-OCTANOL
TetraMethylaMMoniuM hydrogen phthalate, 99+%
3,4-Dichlorobenzaldehyde
3-Bromo-3,3-difluoroprop-1-ene 98%
6-Bromo-2-methyl-2H-indazole
N,N',N''-Tris(p-toluenesulfonyl)diethylenetriamine
4-Chlorobenzonitrile-2,5-diboronic acid neopentyl glycol ester
