WILL-2细胞：人B淋巴瘤复苏快细胞系

WILL-2细胞：人B淋巴瘤复苏快细胞系 货号：GD-C4720038
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
7-Methyl-5,6,7,8-tetrahydrobenzo[b]thieno[2,3-d]pyriMidin-4(3H)-one, 96%
(R)-(-)-2-[Hydroxy(diphenyl)Methyl]-1-Methylpyrrolidine
NICKEL ARSENIDE
N-Acetyl 3,5-dibroMo-2-nitroaniline
(R)-(-)-Benzyl glycidyl ether
PRASEODYMIUM(III) NITRIDE
IODOCYCLOHEXANE
3-Fluorophthalic anhydride
Lithium 2-ethylhexanoate, min.98%
L-Ornithine Dihydrochloride
DL-3-(1-Methyl-1-ethenyl)-6-oxoheptanenitrile, 98%
OXPRENOLOL HYDROCHLORIDE
DL-4-CHLOROPHENYLALANINE ETHYL ESTER HYDROCHLORIDE
2'-Deoxyadenosine 5'-phosphate
1,1'-Binaphthyl-2,2'-diyl hydrogenphosphate
(R)-(+)-1,1'-Bi-2-naphthol
3,5-Dichloro-2,4,6-trifluorobenzotrifluoride
N-Cyclohexyl 2-broMo-5-fluorobenzaMide
Tetraphenylboron sodiuM, ACS reagent
NutMeg Oil, East-Indian Type, FCC
1-Methyl N-Carbobenzoxy-L-glutaMate
Methyl Orange
Vinyltriphenylphosphonium bromide
2,4-Dimethyl-1,3-dioxane
naptalam
1,3-Diphenoxy-2-propanol
4-(DIFLUOROMETHOXY)BENZOIC ACID
5-MethyltryptaMine hydrochloride
Ethyl 3-pyridyloxyacetate, 98%
Phenylglyoxylic acid, 98%
Ethyl α-hydroxyisobutyrate
4-Chloro-1-naphthol (4-CN)
SodiuM butanoate
SesaMe Oil
4-(3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-Heptadecafluorodecylthio)phenol
DisodiuM Anthraquinone-2,6-disulfonate , 98%
4,5-Difluoro-2-methylaniline
Iron(II) iodide, anhydrous (99.5%-Fe)
(Dichloromethyl)trimethylsilane
4-Chloro-3-nitrobenzyl Alcohol
2-AMINO-5-CHLOROTHIAZOLE HYDROCHLORIDE
PYRAZINE-2,5-DICARBOXYLIC ACID
DIMETHYLETHOXYSILANE
1,2,3-Benzenetricarboxylic acid
2-BroMo-1-(2-thienyl)-1-ethanone
OligoMycin B
2-Tetradecanol
1-Tetradecanol, 99%
2,3-Dihydro-1,4-benzodioxine-5-carbonitrile, 97%
Myristoyl chloride
HoMatropine Hydrochloride
Oxadixyl;Sandofan
[(4-methoxyphenyl)methyl] hydrogen (4-hydroxyphenyl)malonate
4-(Trifluoromethyl)-1H-imidazole
Clindamycin
2-(TRIFLUOROMETHYL)PHENYLACETONITRILE
4-CHLORO-2-METHYLPHENYL ISOCYANATE
2-(3-Chlorophenyl)ethylaMine
1-Azido-1-deoxy-β-D-lactopyranoside
Myrcene ,90% [tech., stabilized]
Magnesium hydroxide, 95%
4'-Iodo-2'-(trifluoromethyl)acetanilide
4-(Difluoromethoxy)benzensulfonamide
9-Phenyl-9-fluorenol
METHYL 3-IODO-4-METHOXYBENZOATE
1-(4'-AMINOPHENYL)-1,2,4-TRIAZOLE
DL-2-Octanol
1,3-Dihydro-1-(1,2,3,6-tetrahydro-4-pyridinyl)-2H-benziMidazole-2-one
1,3,5-Cyclohexanetriol (cis- and trans- Mixture)
2-Methylindazole-4-boronic acid
Ethynylcyclopropane, 98%
1,3-Thiazol-2-ylMethanol, 97%
Indigotrisulfonic Acid PotassiuM Salt
cis-1,2-CyclohexanediMethanol
Benzoicacidbiphenylester
hydralazine
(3aR,7aS)-octahydro-1H-isoindole hydrochloride (1:1)
(±)-2,6-Diamino-4,5,6,7-tetrahydrobenzothiazole
AGT ELISA Kit
Mouse interferon-inducible protein 16,IFI16/p16 ELISA Kit
FABP ELISA Kit
Human CAPN5 ELISA Kit
Renin ELISA Kit
Rat PIIINP (N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit
Aβ1-40 ELISA Kit
Human FGFR4 ELISA Kit
Human TMSβ4 (Thymosin Beta 4) ELISA Kit
MLT ELISA Kit
Sabouraud Brain Heart Infusion Agar Base
Glucose Yeast extract Calcium carbonate Medium
Indole-4-carboxylic acid, 97%
Heptanoic Acid
5-AMinonaphthalene-1-sulfonic acid, tech. 90%
2-ISOBUTYL-3-METHYLPYRAZINE
Methyl Orange
Calcium Sulfate Dihydrate
2-Bromo-5-methoxybenzaldehyde
p-Methylaminophenol

SUBEROYL CHLORIDE
1-(2-CHLORO-PHENYL)-PYRROLE-2,5-DIONE
N,N-DiMethylsulfaMide
3-Chloro-2,6-difluorobenzaldehyde, 97%
4-Cyanobenzoic acid, 98%
DMSO-d6
4'-METHOXY-2-PHENYLACETOPHENONE
3-ACRYLOYL-2-OXAZOLIDINONE
1,6-DIBROMO-2-HYDROXYNAPHTHALENE-3-CARBOXYLIC ACID
Boc-D-Valine
4-Fluorophenylacetaldehyde diMethylacetal
Decyl Decanoate
9H-Fluoren-9-ylmethyl 4-aminopiperidine-1-carboxylate hydrochloride
Dichloro[(S)-(-)-2,2'-bis(diphenylphosphino)-1,1'-binaphthyl][(1S,2S)-(-)-1,2-diphenylethylenediamine]ruthenium(II), min. 98%
Sodium 1-Naphthol-5-sulfonate