OCI-LY8细胞：人B淋巴瘤复苏快细胞系

OCI-LY8细胞：人B淋巴瘤复苏快细胞系 货号：GD-C4720037
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,4-Dihydroxybenzaldehyde, 98%
4-AMino-3-Methylbenzonitrile
2-Chloro-5-nitrobenzoyl chloride
AluMinuM iodide, ultra dry, 99.998% (Metals basis)
O-TERT-BUTYLHYDROXYLAMINE HYDROCHLORIDE
AMMONIUM MAGNESIUM PHOSPHATE HEXAHYDRATE
3-BENZYLOXY-4-METHOXYBENZOIC ACID
Trimethylsilylethynyl(phenyl)iodonium Tetrafluoroborate
tert-Butyl Methyl Sulfide
4-Chloro-3-(trifluoromethyl)benzyl bromide
AF-DX 116
Triasulfuron
TRI-N-PROPYLTIN CHLORIDE
1,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8-Heptadecafluoro-10-iododecane
D-1-N-Boc-prolinamide
Isophorone Diisocyanate (Mixture of isoMers)
1,3-Dichloro-2,4,6-trifluorobenzene
Cyclohexyl 5-broMo-2-chlorobenzaMide
TetraphenylarsoniuM chloride hydrochloride, tech.
Nuclear Fast Red
Methyl 2,6-Dihydroxybenzoate
perfringens supplement I
Methyl 3-amino-5-chloro-2-hydroxybenzoate
2,6-Lupetidine;
Dibenzothiophene
Neodymium oxide
1-(1,3-DIOXOLAN-2-YLMETHYL)
5-Methylfurfural
2-Phenylethyl propionate, 97%
3-MethylaMino-1,2-propanediol, 99%
2-Methyl-3-(trifluoroMethyl)phenylacetonitrile, 97%
Mesityl oxide
9-HEPTADECANONE
SesaMol
CUPROUS HYDRIDE TRIPHENYLPHOSPHINE HEXAMER
(2-AMinopyridin-3-yl)Methanol
N-(3-pyridinylmethyl)-1-butanamine(SALTDATA: HCl)
3-Bromo-5-methoxytoluene
4,4'-Methylenebis(N,N-dimethylaniline), 99+%
3-iodo-1H-indazole(SALTDATA: FREE)
4-{[4-(Bromomethyl)phenyl]sulphonyl}morpholine
1,2-DICHLOROHEXAFLUOROCYCLOPENTENE
4-CHLORO-1-NAPHTHOL
2,6-Diethyl-N-(2-propoxyethyl)aniline
L-GlutaMic Acid
3,4-Dichlorophenethyl alcohol
1,4-Dibenzoylbenzene
2-Chloro-4-forMylphenylboronic acid
2-BroMo-1-(2-chloropyridin-4-yl)ethanone, 95%
Tyloxapol
D-(-)-Lyxose
Calcium Citrate anhydrous
N'-(ethylcarbonimidoyl)-N,N-dimethylpropane-1,3-diamine hydrochloride
Bismuth subnitrate
Furylacrylic acid
Hexamethyldisiloxane
2,4,6-TRIS(4-FLUOROPHENYL)BOROXIN
3,5-Pyridinedicarboxylic acid
N-Methyl-4,4′-Methylenedianiline
2-Hydroxypyridine ,98%
1-Bromo-3,4-dichlorobutane
4-(Isopropylthio)benzeneboronic acid
2,4-Difluorophenylmethylsulfone
2,4-DIFLUORO-5-NITROPHENYLBORONIC ACID
(4-FLUORO-PHENYL)-METHANESULFONYL CHLORIDE
2-Chloro-5-methoxyaniline hydrochloride
Diisopropyl succinate
PotassiuM hydrogen fluoride
1,3,5-Benzenetrithiol
2-Chloro-3-(trifluoroMethyl)phenylboronic acid
CeriuM(IV) oxide, 99.9%, (trace Metal basis)
3,5-DibroMo-o-phenylenediaMine Monohydrochloride, 99%
Barbituric Acid
Anisole
Acetylenedicarboxylic acid monopotassium salt
6-Iodo[1,2,4]triazolo[1,5-a]pyridine
9,10-bis(4-methoxyphenyl)-2-chloro-anthracene
3-bromo-5-methyl-pyrazin-2-amine
ACE ELISA Kit
Mouse interferon-inducible protein 16,IFI16/p16 ELISA Kit
Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
Human CAPN6 ELISA Kit
GH ELISA Kit
Rat PCIII (Procollagen III) ELISA Kit
α1-MG ELISA Kit
Human FGFR3 ELISA Kit
Human Thymosin β4 ELISA Kit
SYNPO ELISA Kit
Sabouraud’s Medium with TTC
Yeast extract Dextrose Calcium carbonate Medium
Methyl indole-4-carboxylate
Heptanoic acid, 98+%
5-AMinonaphthalene-2-sulfonic acid, 97%
2-Methyl-3-(Methylthio)pyrazine, 99%
perfringens supplement I
Lead(II) nitrate
Cis-Hexahydrophthalimide
4-ethoxyphenylurea

3-(METHYLSULFONYL)BENZOIC ACID
GLYCEROL MONOISOSTEARATE
2-Cyano-5-chloropyriMidine
4-ForMylphenoxyacetic acid, 98%
4-Ethoxyphenyl 4-n-butylcyclohexanecarboxylate, 97%
5-Methoxyindole-3-carboxaldehyde
2,8-Dichloroquinoline
9-(ChloroMethyl)anthracene
5-Indoleboronic acid pinacol ester
L-GlutaMic acid 5-tert-butyl 1-Methyl ester hydrochloride
2,6-DiaMinopyridine
1H,1H,2H,2H-Heptadecafluorodecyl acrylate, stabilized, 97%
Methyl 4-Fluorobenzoate
3-Fluoro-2-iodopyridine
Methyl 2,4-dichlorothiazole-5-carboxylate