A375SM细胞：人恶性黑色素瘤复苏快细胞系 货号：GD-C4718262
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Ethoxy-2-(MethylaMino)pyridine, 98%
5-(2-Hydroxyethyl)aMino-o-cresol
2,2,2-Trifluoro-2′,4′-diMethoxyacetophenone
2,5-Dichlorophenyl isothiocyanate
1-(5-Methyl-2-pyridinyl)
(S)-1-PHENYL-2-(P-TOLYL)ETHYLAMINE
Xanthane Hydride , 95%
4-Nitrophenyl isothiocyanate
5-Methyl-3-(4-methyl-1-piperazinyl)-5H-pyridazino[3,4-b][1,4]benzoxazine
3-Nitro-2-pentanol, mixture of(+/-)-threo and (+/-)-erythro
4-[Cyclohexyl(methyl)carbamoyl]benzeneboronic acid 98%
Boron trifluoride
2-AMINO-4'-CHLORODIPHENYL SULFIDE
2,5-DIMETHOXY-BETA-NITROSTYRENE
[1,3-Bis(diphenylphosphino)propane]nickel(II) chloride
6-Morpholino-3-pyridinyl isothiocyanate
N-ButyldiMethylaMine
4-BroMo-2-Mercapto-6-Methylbenzo]thiazole
Pivaldehyde, 97%, AcroSeal
Fast Blue BB Base
Methyl 6-Hydroxy-2-naphthoate
(-)-Lobeline Hydrochloride
2-Chlorobenzyl cyanide
1,6-anhydro-β-D-glucose
Tolbutamide
Sodium perchlorate
3-Fluoro-5-(trifluoromethyl)benzoic acid
Pyridine
RutheniuM(III) chloride hydrate, PreMion|r, 99.99% (Metals basis)
1-(2-Methylbenzyl), 97%
Phenyl vinyl sulfide
4,4′-Thiodiphenol
FMoc-(S)-(-)-piperidine-2-carboxylic acid
Z-Val-ONp
IridiuM tetrachloride
2,6-Difluoro-3-propoxyphenylboronic acid
1-(4-Cyanophenyl)-2-thiourea
4-Fluoropyridine Hydrochloride
4-Bromo-3-chlorobenzenesulfonyl chloride
H-Met-OMe.HCl
4-Isopropylaniline
6-CHLORO-4-HYDROXYQUINOLINE-3-CARBOXYLIC ACID
2,5-Dimethoxynaphthalene
sodiuM hydride dry
1-(2-AMinoethyl)-2-Methyl-5-nitroiMidazole dihydrochloride Monohydrate
Copper(II) acetate Monohydrate
4-Methoxybenzhydrol
2-Oxo-2,3-dihydro-1H-iMidazole-4-carboxylic acid
(2S,5S)-1-(2-(1,3-Dioxolan-2-yl)phenyl)-2,5-diMethylphospholane, 97%
(2S,5R,6R)-6-[(R)-2-Amino-2-phenylacetamido]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid
1H,1H,5H-Octafluoropentyl Acrylate (stabilized with MEHQ)
Adogen
2,3,3-Trimethyl-5-Methoxy-indolenine
Glybenzcyclamide
Crotononitrile (cis- and trans- mixture)
GALLIUM(III) TRIFLUOROMETHANESULFONATE
DL-O-METHYLSERINE
2-Chloro-6-Methylpyridine-4-carboxylic acid
Etifenin
3-tert-Butylsulfanyl-pyridine-2-carbonitrile
Fullerene C60, 99.9%
Glycidyl phenyl ether, 99%
6-methyl-2,3,4,9-tetrahydro-1H-carbazol-1-one(SALTDATA: FREE)
1,8-Dihydroxy-4,5-dinitroanthraquinone
3-Methoxy-2-methylaniline
Folpet
Acetylcholine Chloride
3-NitrophthaliMide
(-)-BenzotetraMisole
2-Ethylhexyl 3-Mercaptopropionate
2'-Deoxyuridine 5'-Monophosphate disodiuM salt
3-Methoxyphenethyl alcohol, 97%
3-Bromo-1-propanol
Dodecafluorosuberic Acid
2-Bromo-5-nitroaniline
4-methoxypyridine-2-carboxylic acid
Casein Sodium
2,5-Dibromothiazole
t-PA ELISA Kit
FOXM1 ELISA Kit
Human LTBP2 ELISA Kit
Human Bone morphogenetic protein receptor 1A,BMPR-1A ELISA Kit
KL-6 ELISA Kit
OC ELISA Kit
AT2R ELISA Kit
Human CASP3 (Caspase 3) ELISA Kit
CVL ELISA Kit
AHA ELISA Kit
Triple Sugar Iron Agar Medium
Sodium malonate Medium
PALMITOLEIC ACID METHYL ESTER
SULFACHLOROPYRIDAZINE
5-BroMo-2-Chloronicotinic Acid
Methyl 2-Hydroxyisobutyrate
(-)-Lobeline Hydrochloride
tantalum(5+) ethanolate
bromopentamethylbenzene
Methylglyoxal
3,5-Difluorophenol
TWEEN 85
1-Methoxynaphthalene
1,1'-Bis[(2S,5S)-2,5-diMethyl-1-phospholanyl]ferrocene, 97+%
^a-Hydroxy-^a-Methylbenzyl phenyl ketone, 98%
2-METHOXY-6-NITRO-PHENYLAMINE
1-[2-(DiMethylaMino)ethyl]-1H-tetrazole-5-thiol
3-BroMo-2,4,6-triMethylphenyl isocyanate
2-AcetaMido-4-Methylphenol
FMoc-Lys(Tfa)-OH
5-Phenylhydantoin , 99%
(S)-(+)-4-Phenylbutan-2-ol
Methyl Undecafluorohexanoate
L-Leucine Ethyl Ester Hydrochloride
1-Acetyl-5-bromoindolin-3-one
