IEC-6[IEC6]细胞：大鼠小肠隐窝上皮复苏快细胞系

IEC-6[IEC6]细胞：大鼠小肠隐窝上皮复苏快细胞系 货号：GD-C4720456
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,4-Dichloro-3-ethyl-6-nitrophenol, 98%
AluMinuM acetate basic
(S)-(-)-2-BROMOPROPIONIC ACID
N-(HydroxyMethyl)acrylaMide , 98%
(11S,12S)-9,10-DIHYDRO-9,10-ETHANOANTHRACENE-11,12-DIAMINE
2-Hydroxy-2-Methylbutyric acid
5-Methyl furfural
2,5-Dichlorothiophene-3-sulfonyl chloride ,97%
Cerium(IV) trifluoromethanesulfonate, 99%
N,N-Bis-(2-chloroethyl)-p-toluenesulfonamide, tech., 90%
N-Methylmethacrylamide (stabilized with HQ)
GLUTARYL DICHLORIDE
ALL-TRANS-RETINAL
BROMOPENTAMETHYLBENZENE
Orange II
DL-PyroglutaMic acid
6,6'-DibroMo-2,2'-bipyridyl
Methyl 6-broMonicotinate
1,7,7-TriMethylbicyclo[2.2.1]heptan-2-one oxiMe, 97%
Tris(2-carboxyethyl)phosphine Hydrochloride
2-Propylcyclohexanol (cis- and trans- Mixture)
5-Fluoro-1-indanone
Acid Yellow 25
methyltrimethoxysilane
p-Hydroxycinnamic acid
(R)-1-(2-BROMOPHENYL)ETHANOL
3-METHYL-2(5H)-FURANONE
Cis-3-Hexenyl Butyrate
2-AMino-3-fluorobenzonitrile, 95%
5-AMino-2-(trifluoroMethyl)benziMidazole, 97+%
2-Methoxynaphthalene, 98%
2-BroMo-4-tert-butylphenol
EuropiuM(III) oxalate hydrate, 99.9% (REO)
N-tert-Butyl-4-MethoxybenzaMide
4-AMINOBENZOPHENONE
3-Acetyl-2-fluorophenylboronic acid
DL-PHENYLALANINOL
3-Quinuclidinone hydrochloride, 99%
2,6-Dichlorophenylacetonitrile, 97%
2-Amino-4-hydroxybenzothiazole
D-1-Cbz-Nipecotic acid
3-(2,6-Dichlorophenyl)-5-methylisoxazole-4-carboxylic acid
L-TARTARIC ACID DIBENZYL ESTER
5-BROMO-2-IODOBENZONITRILE
2,6-Dichlorobenzoyl chloride
Ethyl 2-aMino-4-phenyl-5-thiazolecarboxylate
DiphenyliodoniuM Hexafluorophosphate
1-(3-BroMophenyl)cyclopropanecarbonitrile
Silver phosphate, 98%
L-HoMocystine
DiMethyl Tetrachloroterephthalate
4-amino-3-(trifluoromethyl)benzoic acid
Methacycline Hydrochloride
5-methylbenzimidazole
2-Ethylhexyl acetate
2-PHENYLINDOLE-3-ACETONITRILE
1,4-DIBROMO-2-BUTANOL
DL-2-AMINOOCTANOIC ACID
7-BroMobenzofuran-2-carboxylic acid
Benzyl Carbazate
alpha-Angelicalactone
1,2-Bis(2,3,4,5,6-pentabromophenyl)ethane
L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt ,98%
Erlotinib hydrochloride
2-Iodopropane
p-Hydroxy-cinnamic acid
Methyl 3-isoquinolinecarboxylate
4-(Methoxycarbonyl)phenylboronic Acid
1-Butyl-3-MethyliMidazoliuM Trifluoro(trifluoroMethyl)borate
3-(3-(Ethoxycarbonyl)piperidine-1-carbonyl)phenylboronic acid
Decane, 99+%, pure
cis-1,4-Diacetoxy-2-butene, 95%
4-Nitrobenzhydrazide
2,5-Diphenyl-1,4-benzoquinone
4,4'-dimethyl-2,2'-dipyridyl
3-Bromo-6-chloro-2-pyridinecarboxylic acid
t-Butyl(E)-3,5-dihydroxy-7-[3'-(4''-fluorophenyl)-1'-methylethyl-indol-2'-yl]-6-heptenoate
rosarin
EAR12 ELISA Kit
P-GP ELISA Kit
Human PRM2 ELISA Kit
Human DHODH ELISA Kit
1,3-βD glucosidase ELISA Kit
Rat β-CTx (Beta Crosslaps) ELISA Kit
Chicken Immunoglobulin G,IgG ELISA Kit
Human PCI (Protein C Inhibitor ) ELISA Kit
Human 50% complement hemolysis,CH50 ELISA Kit
FFA ELISA Kit
Andrade′s Carbohydrate Broth Base
Power-nitrate Medium
LutetiuM(III) 2,4-pentanedionate, REacton|r, 99.9% (REO)
CalciuM fluoride, 99%
5-(Chlorosulfonyl)salicylic acid, 97%
2-(Dicyclohexylphosphino)-2,4,6-Triisopropylbiphenyl
5-Fluoro-1-indanone
Deflazacort
Fluorographite polymer
hexacosanoic acid

Tetraisopropyl methylenediphosphonate
SODIUM 4-N-OCTYLBENZENESULFONATE
1-BroMo-5-Fluoropentane
HoMoMorpholine hydrochloride, 98%
^b-Naphthoflavone, 98+%
EDTA dipotassiuM salt
3-Ethylaniline
1-BroMo-3-fluoro-5-pyrrolidinobenzene
2-BroMoMethyl-4-fluorophenylboronic acid, neopentyl glycol ester
Farnesal, Mixture of isoMers
N-(2,6-DiMethylphenyl) 2-broMobenzaMide
Conduritol B epoxide
4-Bromo-2-methoxypyrimidine
2,4-Difluorobenzophenone
4-Amino-2-chloro-5-fluoropyrimidine, 98%