A-375细胞：人恶性黑色素瘤复苏快细胞系

A-375细胞：人恶性黑色素瘤复苏快细胞系 货号：GD-C4718363

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
造成实验室细胞污染常见情况总结：细胞培养中Z常见污染的是细菌、真菌和支原体污染。细胞一旦污染，大多数较难处理。那么，哪些情况我们不注意的话就会造成细胞污染呢？我们根据常见细胞培养实验分析总结下。【违规操作】1)为节省时间，有人已经用超净台四个多小时，不开紫外灭菌30min,酒精擦拭后直接开始试验；2)器材或者溶液很久没用，未检测是否污染而直接使用；离心管多次使用，枪头为了方便交叉使用；3)超净台不点酒精灯；点了酒精灯放在右上角，而你在左下角做试验；4)不带手套，徒手操作；5)细胞培养间配备枪式移液器、手术器械、离心机、冰箱等专用仪器设备以及专用的实验服和拖鞋，未定期消毒。专用物品被带出传代细胞使用。培养细胞过程中使用的所有实验用具，如移液管、一次性枪头、一次性塑料离心管、冻存管等未按要求灭菌使用(通常需121°C高压灭菌20分钟后37％烤干备用)。超净台和桌面，东西太多太乱：超净台不是储物箱，什么培养皿、各种规格的板子、枪头就不要堆在超净台！这样就会有许多紫外线顾不到的卫生死角。传代细胞其他的桌面，切忌东西堆积如山，不要将酒精棉球、标签纸、牛皮纸买来后全部堆在传代细胞！一不小心“飘”进你的细胞培养板里，细胞就会养的不好，啥时候死了都不知道！【培养箱太久没清洁】细胞污染了，并非直接扔了培养皿就不管了，首先你还得看看这个恒温培养箱里其他培养皿或孔板里的细胞是否污染，如果有而且好几个板子都有类似的污染块，那很可能是培养箱中的水或者空气污染了，得给培养箱做个大扫除，重新酒精消毒，照紫外；孵箱里的水，水没了要记得加，还得记得十天半个月的就用酒精擦擦托盘。【传代细胞人多口杂，难管理】在传代细胞这种卫生要求高，人多了，不确定因素多了，难以保证试验在无菌条件下操作。出入试验室，实验服当风衣穿，不扣纽扣，不戴鞋套，就容易造成细胞污染；超净台做实验时，喜欢说话聊着做试验，要是还不带口罩，里面就有很多细菌等着去攻击你的细胞呢！
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Cyclopropyl-4-diMethoxyMethylpyriMidine, 97%
Parathyroid horMone huMan
2-(4-CHLOROPHENYL)INDOLE
D-(+)-MELEZITOSE
4-(DIFLUOROMETHOXY)BROMOBENZENE
2-HYDROXY-4-METHOXYANILINE HYDROCHLORIDE
SodiuM 2,3-diMercapto-1-propanesulfonate
Tin(IV) t-butoxide (99.99%-Sn) PURATREM
Acetylacetonato(norbornadiene)rhodium(I)
2-(4-Diethylamino-2-hydroxybenzoyl)benzoic Acid
2-Hydroxy-2-(trifluoromethyl)butyric acid 97%
(-)-2-Amino-4-phenylbutyric acid
Methyl 5-bromonicotinate
Diallyl bisphenol A
(S)-(-)-1,1'-Bi-2-naphthol
3'-Chloro-5'-fluoroacetophenone
1,4-Diacetoxybenzene
5-BroMo-1-Methyl-1H-iMidazole-2-carbaldehyde
PotassiuM thioacetate, 98%
Gold (I) Chloride
4-(TrifluoroMethoxy)benzaldehyde
7H-benzo[c]carbazole
N-gamma-ethyl-L-glutamine
α-Bromo-4-chloroacetophenone
Tetrabromobisphenol A
Nicotine ditartrate
2-Amino-5-chlorobenzoic acid
4-Chloro-6-Methylquinoline
1-HydroxyMethyl-5,5-diMethylhydantoin, 97%
Ethyl 2-oxopyrrolidine-3-carboxylate, 95%
5-AMinouracil, 97%
PalMatine hydrochloride
6-METHOXY-4-METHYLCOUMARIN
Tris(triphenylphosphine)rutheniuM(II) chloride
3,5-DIMETHYLCYCLOHEXANOL
2-(DiMethylaMino)pyridine-5-boronic acid, hydrade
Tin(IV) bis(acetylacetonate) dibroMide
Ethoxycarbonyl Isothiocyanate
2,4-Dimethoxythiobenzamide
1-Boc-3-methylene-piperidine
4-Chloro-3-methylpyridine hydrochloride
DL-1-(Aminoethyl)phosphonic acid
4,4,4-TRIFLUOROCROTONIC ACID
YTTERBIUM(III) CHLORIDE HEXAHYDRATE
3,4-Dimethoxy-3-cyclobutene-1,2-dione
4-BroMo-2-fluorobenzonitrile
4-Diazo-3-MethoxydiphenylaMine Sulfate
1-(2-BroMophenyl)-3-Methylthiourea
SodiuM cyanoacetate, 50 wt% aqueous solution
L-Methionine Sulfone
5-Chloro-3-Methylbenzo[b]thiophene
1-Fluoro-3-nitrobenzene
γ-Methacryloxypropyl methyl dimethoxy Silane
5-Methylhydantoin
cinnamyl isobutyrate
2,6-BIS(P-TOLYL)PYRIDINE
AMylose (Mw.=ca. 2,800)
2',5'-DICHLOROACETOACETANILIDE
Tetrahydrothiopyran-4-one
(1-Methyl-1H-indol-4-yl)methanol
4-Amino-2-methylbenzoic acid
1,2-Dibromobutane, 97%
Dichlorodiphenylmethane, 99%
1-NITROANTHRAQUINONE
Trimethylaluminium
Cyclohexanone oxime
ArteMether Related CoMpound B;(3R,5aS,6R,8aS,9R,10R,12R,12aR)-Decahydro-10-Methoxy-3,6,9-triMethyl-3,12-epoxy-12H-pyrano[4,3-j]-1,2-benzodioxepin
4-Chlorofuro[3,2-c]pyridine
(1S,2S)-1-AMino-2-(benzyloxy)cyclopentane
4-Acetoxyphenylboronic acid, pinacol ester
4-Chlorophenylhydrazine hydrochloride, 97%
1,6-Dicyanohexane, 99%
(2S,3S)(-)-Dihydroxybutane-1,4-dioic acid diethyl ester
5-BroMopentyl Acetate
allylbenzene
N,N'-Di(1-naphthyl)-4,4'-benzidine
N-(3,4-Diethoxyphenylethyl)-3,4-diethoxyphenylacetamide
boc-L-phenylalanine methyl ester
MHC I/RLA I ELISA Kit
PDHa ELISA Kit
PCX ELISA Kit
Human QPCT ELISA Kit
MPO ELISA Kit
Rat 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit
KLH ELISA Kit
Human SEPT6 ELISA Kit
Human CCND2 ELISA Kit
SCCAg2 ELISA Kit
Modified KIA Iron Medium
D2a Medium
Chicago Sky Blue 6B
CyclopentadienylniobiuM(V) tetrachloride
5-Nitroindoline, 97%
2-Hydroxy-4-n-Octyloxybenzophenone
7H-benzo[c]carbazole
4,4-diethoxybutylamine
4-Hydroxy-3-nitropyridine
5-Methylsalicylic acid

1-(2-BROMOETHOXY)-2-FLUOROBENZENE
2,4-Dinitro-1-(trifluoromethoxy)benzene
6-Heptyn-1-ol
1-Methyl-2-(tri-n-butylstannyl)pyrrole, 98%
2-Fluoropyridine, 99%
Benzanilide, 98+%
3,3,4,4,5,5,6,6,7,7,8,8,8-Tridecafluorooctyl Methacrylate
4-(2-Thienyl)benzaldeyde
1-[4-(TRIFLUOROMETHYL)PHENYL]ETHANOL
TETRAETHYLGERMANE
EATON'S REAGENT
Methyl 4-Methoxysalicylate
2-Amino-thiazole-4-carboxylic acid
Methyl Hexanoate
Lanosterol