HT1080(HT-1080)细胞:人纤维肉瘤复苏快细胞系 货号:GD-C4718293
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Fluoro-4-(4-MethoxybenzyloxycarbaMoyl)benzeneboronic acid, 98%
[6,6]-Phenyl C61 butyric acid Methyl ester
FORMALDEHYDE 2,4-DINITROPHENYLHYDRAZONE
Methyl 5-cyclopropyl-1-phenylpyrazole-4-carboxylate
NITRONIUM HEXAFLUOROANTIMONATE
(-)-(5aS,10bR)-5a,10b-Dihydro-2-(2,4,6-triMethylphenyl)-4H,6H-indeno[2,1-b][1,2,4]triazolo[4,3-d][1,4]oxaziniuM Chloride Monohydrate, 98%
BIS(4-METHOXYPHENYL) DISULPHIDE
L(-)-Glutathione, oxidized, hydrate, 95%
2,2-Bis(trifluoromethyl)propanol 97%
h-ser.otbu.hcl
Methyl 4-(acetylamino)-2-hydroxybenzenecarboxylate
3-Chloro-1,2-benzisothiazole
4-Bromo-1-butene
4-Chloro-3,5-difluorobromobenzene
3-Chloropropanesulfonyl chloride
SodiuM phosphate
1,1-Dichloro-2-propanone
2-BroMo-1-Methoxy-3-Methylbenzene
Poly(tetrafluoroethylene)
MagnesiuM Silicate
4-Benzoylbutyric Acid
4-Phenylphenacyl bromide
2-Iodoimidazole
1,1'-Carbonyldiimidazole
3,6-Dimethyl-4-octyn-3,6-diol
2,6-Difluorobenzyl chloride
P-TOLUENESULFONYL FLUORIDE
Fluorescein DisodiuM Salt
Methylenediphosphonic acid, 99+%
2-Methoxyethyl chloroforMate, tech. 85%
Vinorelbine Ditartrate salt hydrate
FMoc-D-His(Trt)-OH
4-CHLORO-2,6-DIFLUOROPHENYLACETIC ACID
Zinc broMide
Methyl 3-Pentenoate , 90%
Furylacrylic acid
D-Phenylalaninol
(6-(1H-Pyrazol-1-yl)pyridin-3-yl)methanol ,97%
Zinc sulfate heptahydrate, for analysis ACS, 99%
(4-methoxybenzyl)(4-methylbenzyl)amine(SALTDATA: HBr)
FMOC-(S)-3-AMINO-4-(2-METHYL-PHENYL)-BUTYRIC ACID
Octadecanamide
4-(Trifluoromethyl)-2'-biphenylcarboxylic acid
DOWANOL(TM) PNB
Glycolic acid
Tetracycline hydrochloride
Tris(2-acryloyloxyethyl) Isocyanurate (stabilized with Phenothiazine)
2-Furonitrile, 99%
Dicyclohexylphenylphosphine, 95%
4-CHLORO-2-ISOPROPYL-5-METHYLPHENOL
6-AMino-2,4-lutidine
1H-Pyrazole, 3-bromo-1-methyl-
3,5-Dichloro-4-Picoline
2-(4-iodophenyl)ethanol
3,11-dichloro-6,11-dihydro-6-methyldibenzo[c,f][1,2]thiazepine 5,5-dioxide
3,4-Dibromothiophene
1-CHLORO-1-PROPENE
1-Naphthoic acid
4-(N-BenzylaMinocarbonyl)phenylboronic acid
n-Hexyl sulfonyl chloride
(1R,2R)-N,N'-Bis[3-oxo-2-(2,4,6-trimethylbenzoyl)butylidene]-1,2-diphenylethylenediaminato Cobalt(II)
Potassium (3-methanesulphonylaminophenyl)trifluoroborate
1-(tetrahydro-2-furanylmethyl)-1H-pyrrole-2-carbaldehyde(SALTDATA: FREE)
5-Bromoisatoic anhydride
S,S'-Dimethyl dithiocarbonate
Isoalantolactone
3',4'-DICHLOROACETOPHENONE
4-Methyl-2-phenyl-1,3-thiazole-5-sulfonyl chloride
(+)-Citronellal
3-Isopropylphenylboronic acid, pinacol ester
DysprosiuM(III) trifluoroMethanesulfonate, 99%
3-(5-BroMo-2-thienyl)-5-Methyl-1,2,4-oxadiazole, 97%
tert-Butyl Acetoacetate
1,5-Naphthalenebis(trifluoroMethanesulfonate)
5-Methyl-1H-Indazole
Chlorobutanol
docetaxel trihydrate
2-(2H-benzotriazol-2-yl)-4-methyl-6-(2-methylprop-2-en-1-yl)phenol
TP ELISA Kit
CFH ELISA Kit
Human CTSK ELISA Kit
Human BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit
5HT ELISA Kit
VLDL ELISA Kit
BK ELISA Kit
GAL14 ELISA Kit
Human Cytochrome P450c21/21-hydroxylase,CYP21A ELISA Kit
SRAGE ELISA Kit
Letheen Agar Base, Modified
AA Fluid Medium
HOLO-TRANSFERRIN
BERBERINE CHLORIDE
5-BroMo-2,4-Difluoroaniline
6-Hydroxypyridine-3-boronic acid pinacol ester, 97%
4-Phenylphenacyl bromide
Brilliant Blue G
4-[cyclohexylidene(4-hydroxyphenyl)methyl]phenol
dithiooxamide
NICKEL ARSENIDE
3-Indoleacetonitrile
3-[N-Tris(HydroxyMethyl)MethylaMino]-2-Hydroxypropanesulfonic Acid SodiuM Salt
4-Benzyloxy-3-Methoxyphenylacetonitrile, 97%
2,6-DiaMinopiMelic acid, 96%
(±)-1,2-DiphenylethylenediaMine
Inosine-5'-Monophosphate disodiuM salt octahydrate
1-ACETYLISONIPECOTOYL CHLORIDE
2-CHLORO-2,2-DIPHENYLACETYL CHLORIDE
4-TERT-BUTOXYPHENOL
1-BroMo-2-isopropoxybenzene, 97%
2-Fluoro-4-iodobenzonitrile
Caesium chloride, optical grade 99.999%
2-Naphthoxyacetic acid, 98%
5-Bromo-3-carboxy-2-fluorobenzeneboronic acid