MES-13细胞：小鼠肾小球系膜复苏快细胞系

MES-13细胞：小鼠肾小球系膜复苏快细胞系 货号：GD-C4719691
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-tert-Butyl-6-Methyl-phenol
PiriMicarb
BISMUTH (III) TERT-PENTOXIDE
PyridiniuM toluene-4-sulphonate
Oxalyl chloride
N-t-Boc-L-proline-N-hydroxysucciniMide Ester
2-Nonene (cis- and trans- mixture)
Diprophylline ,99%
3-Quinolinecarbonitrile ,98%
2-Fluoro-6-methylbenzonitrile
3-(DIMETHYLAMINO)PYRROLIDINE
1-(5-Chloro-2-hydroxyphenyl)ethanone
Isobutyl vinyl ether
HYDROGEN SULFIDE
4-(DiMethylaMino)-1,1-diMethoxybut-3-en-2-one
3,6-Difluoropyridine-2-carboxylic acid
3-(EthylaMino)phenol HeMisulfate
3-AcetaMido-6-chloropyridazine
4-(Piperidinosulfonyl)aniline, 97%
DIBENZ(AH)ANTHRACENE
Ethyl Thioglycolate
3,4-Dichlorofluorobenzene
Gum acacia
2-Isopropylnaphthalene
4-Ethoxybenzoic acid
3-Bromothioanisole
Guanine
4,5-Difluoro-2-Nitroaniline
cis-2,6-DiMethyl, 99+%
N-Boc-L-phenylalaninol, 99%
2-Nitrobenzyl broMide
Dibenzyl ether
1-(3-Methoxyphenyl)
N,N′,N′′-TriMethylbis(hexaMethylene)triaMine
2-Chloro-1,3-dinitrobenzene
(R)-Boc-4-Methyl-β-Phe-OH
4-Bromo-2,3-difluorophenol
2-ethylpyrimidine-5-carbaldehyde(SALTDATA: FREE)
Phenylthioacetonitrile
Hematein
3-Phenoxy-benzaldehyde
DIMETHYLZINC
3,5-PYRAZOLEDICARBOXYLIC ACID
isophorone diisocyanate
3,5-Dichloro-4-(2-chloro-4-nitrophenoxy)benzene-1-sulfonyl chloride
4-AMino-5-chloro-2,1,3-benzothiadiazole
2-Chloro-6-nitropyridine
4,5-DibroMothiophene-2-sulfonyl chloride, 97%
(2R,5R)-2,5-Hexanediol, 95%, 98% ee
4,4'-DIHYDROXYSTILBENE
(2-CarbaMoylphenoxy)acetic Acid
4-Heptanone
(SP-4-2)-[(1R,2R)-1,2-Cyclohexanediamine-kappaN,kappaN'])bis(myristato-kappaO)platinum(II)
tebbe
3-Cyanobenzenepropanoic acid
(S)-2-(Boc-amino)-3-methylbutyric acid
3-Nitro-o-xylene
6-BroMo-(4-MethoxybenzylaMino)pyridine
4-Methoxyphenyl Mesylate
Methyl 3-fluoro-4-hydroxybenzoate
1-(3,4-Dimethylphenyl)hydrazine hydrochloride , 97%
4-Amino-3-nitrobenzophenone
Tetraamylammonium Iodide
2-Cyano-N,N-diethylacetamide
3-(1,1,2,2-TETRAFLUOROETHOXY)BENZALDEHYDE
4-Hydroxy-3-methoxybenzylamine hydrochloride
1，3-Cyclopentadiene
3,3-DiMethylacryloyl chloride
2,2'-Dipyridyl disulfide
N-(9-DiMethylaMino-1-oxononyl)-N-hydroxy-beta-alanine Methyl Ester
Methyl 2-broMothiazole-4-carboxylate
Betaine hydrochloride, 99%, extra pure
ThiMerosal, USP
Oleyl Chloride
Alpha'-Chloro-Alpha,Alpha,Alpha-trifluoro-m-xylene
1,8-Diazabicyclo[5.4.0]undec-7-ene
Copper(II) oxide
1,11-Undecanedicarboxylic acid
Porcine Endothelial nitric oxide synthase 3,eNOS-3 ELISA Kit
Apelin ELISA Kit
Human IGFBP-2 (Insulin Like Growth Factor Binding Protein 2) ELISA Kit
Human LMP7/PSMB9 ELISA Kit
IAA ELISA Kit
Rat CD30 (Cluster of differentiation 30) ELISA Kit
Cu/Zn-SOD/SOD1 ELISA Kit
BCAN ELISA Kit
Human INSL3 ELISA Kit
HPCⅢ ELISA Kit
Bean Broth Medium
Free Biotin Assay Medium
Ethyl (S)-1-Boc-5-oxopyrrolidine-2-carboxylate, 98%
trans-2-Fluoro-4-(4-n-propylcyclohexyl)benzonitrile, 97%
4-Ethylphenylacetylene, 99%
2-AMinophenol-5-(N,N-DiMethyl)SulfonaMide
3,4-Dichlorofluorobenzene
2,6-DICHLORO-4-HYDROXYPYRIDINE
Ammonium paratungstate
Diethyl dibromomalonate

TRANS,TRANS-2,4-HEPTADIENAL
2,4,5-TRIMETHYLPHENYLBORONIC ACID
1-(Benzenesulfonyl)-1H-Pyrrolo[2,3-b]Pyridine-3-Boronic Acid Pinacol Ester
ScandiuM(III) sulfate hexahydrate, REacton|r, 99.9% (REO)
(TrifluoroMethoxy)benzene, 99%
2,6-DifluorobenzylaMine
Tricyclohexylphosphine tetrafluoroborate
NALPHA-FMOC-L-SERINE TERT-BUTYL ESTER
Hydrogen peroxide
3-ETHYLPHENYL ISOCYANATE
5-Methyl-2-furanMethanaMine
3-Fluoro-4-nitrobenzotrifluoride 98%
2-Amino-1,3-benzothiazole 98%
2-Amino-5-fluorobenzonitrile
1-Propynylmagnesium bromide, 0.5M solution in THF, AcroSeal