MC53细胞：小鼠肾系膜复苏快细胞系

MC53细胞：小鼠肾系膜复苏快细胞系 货号：GD-C4719686
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
D-(?)-Erythrose
O-tert-Butyl-D-tyrosine
LUTETIUM(III) IODIDE
Phenyl 3-hydroxy-2-naphthoate
TRANS-2,4-DICHLOROCINNAMIC ACID
DipotassiuM Glycyrrhizinate Hydrate, 75%
2,4-Difluorothiophenol 97%
2-Naphthaldehyde
1-Octadecene
3-(2-Chloro-phenyl)-3-oxo-propionic acid ethylester
Copper(II) borofluoride
2,6-DICHLORO-4-METHYLNICOTINAMIDE
Trimethoxyboroxine
2-[N-(2-Cyanoethyl)anilino]ethyl acetate
Octyl 4-MethoxycinnaMate
Diethyl diMethylMalonate
1-Ethoxy-4-hexyloxybenzene
4-Chloro-3-fluorophenylacetic acid
5-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)-1H-indole, 97+%
DI(ETHYLENE GLYCOL)
N-MethylMaleiMide
Ethylenediamine Tetramethylenephosphonic acid
Halquinol
5-Fluoro-2(1H)-pyrimidinone
alpha,alpha-dibromo-4-nitrotoluene
TETRAKIS(ACETONITRILE)PALLADIUM(II) TETRAFLUOROBORATE
POTASSIUM TETRABORATE TETRAHYDRATE
Span 40 (=Sorbitan MonopalMitate)
(R)-3-(FMoc-aMino)-3-phenylpropionic acid, 95%
TriMethylphosphine oxide
Iron(II) chloride
n-Dodecyl-β-D-ThioMaltopyranoside
Methyl 5-(3-chlorophenyl)isoxazole-4-carboxylate
N,N'-BIS(3-AMINOPROPYL)-1,3-PROPANEDIAMINE
Ethyl 5-broMonicotinate
2,4-DIAMINO-5-FLUOROQUINAZOLINE
2-Hydroxy-3-{[2-hydroxy-1,1-bis(hydroxymethyl)-ethyl]amino}-1-propanesulfonic acid
4-Mercaptohydrocinnamic Acid
(R)-(+)-DBD-Pro-COCl [=(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
2-Chloroethyl Benzoate
4-PHENOXYBENZOPHENONE
ENALAPRILAT DIHYDRATE
Squalane
Allopurinol;HPP
DL-Ethyl 2-broMovalerate
5-Acetyl-3-chloro-10,11-dihydrodibenzo[b,f]azepine
2-AcetaMido-4-fluorophenylboronic acid, pinacol ester
4-Chlorobenzoic acid, 99%
(S)-1-BOC-3-Methyl, 98%
Potassium perchlorate
3-tert-Butylsalicylaldehyde
phorate
Microcrystalline Cellulose
5,6-dichloro-1,3-dihydro-2H-indol-2-one
3-bromo-2-(methylthio)pyridine
Cbz-D-Valine
4-Chlorobenzophenone
TRI-N-NONYLAMINE
4-BroMo-1-t-butylpyrazole
3-Hydroxymyristic Acid
6-Chloro-1,3,5-triazine-2,4-diamine
DL-?-(4-Hydroxy-3-methoxy-phenyl)alanine
N'-Hydroxy-2-thiophenecarboximidamide
ALUMINUM POTASSIUM SULFATE
CHLORMEPHOS
5-PYRIDIN-2-YLTHIOPHENE-2-CARBALDEHYDE
Neodymium(III) nitrate hydrate
(4-Methyl-2-thienyl)MethylaMine
2,2-DiMethylglutaric acid
Methyl 3-Methyl-2-nitrobenzoate
N,N-Diethyl 3-nitrobenzenesulfonaMide
N-tert-ButylcyclohexylaMine, 97%
TOS-ARG-OME HCL
(11R,12R)-9,10-Dihydro-9,10-ethanoanthracene-11,12-diaMine
2,6-Dimethyloct-7-en-2-ol
trans-4-n-Pentylcyclohexanecarboxylic acid
Yttrium Fluoride
cyclobenzaprine hydrochloride
EL ELISA Kit
ABCA1 ELISA Kit
Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit
Human low density lipoprotein，LDL ELISA Kit
LCN2/NGAL ELISA Kit
Rat CD109 (Cluster Of Differentiation 109) ELISA Kit
ECF ELISA Kit
Human TERF1 ELISA Kit
Human Insulin receptor substRate 2,IRS-2 ELISA Kit
PⅡCP ELISA Kit
ISP Medium4(Inorganic Salts-Starch Agar)
Biotin Assay Medium
Ethyl 1,3-diMethyl-1H-pyrazole-5-carboxylate, 95%
trans-2-Methyl-2-pentenoic acid, 97%
4-Ethylcyclohexanone, 99%
2-AMinobenzenesulfonaMide
Ethylenediamine Tetramethylenephosphonic acid
2-chloro-6-methoxypyridine
phytic acid dodecasodium from rice
Tetraphenylmethane

2-(2-PYRIDYL)BENZIMIDAZOLE
2-BROMO-6-FLUOROPHENYLBORONIC ACID
2-Nitrophenol SodiuM Salt
Methyl 4-nitropyrrole-2-carboxylate, 95%
4,6-Dichloro-5-MethylpyriMidine, 95%
2,3-Dichlorophenol
4-Fluoro-1-iodo-2-nitrobenzene
1,3-DIPHENYLTRIAZENE
MESITYLGLYOXYLIC ACID
4-(9H-FLUOREN-9-YLMETHOXYCARBONYLAMINO)-PIPERIDINE-1,4-DICARBOXYLIC ACID MONO-TERT-BUTYL ESTER
2,3-Bis(triMethylsiloxy)-1,3-butadiene
3-Phenylumbelliferone 7-O-phosphate hemipyridinium salt
z-glu-otbu
2-[(Trifluoromethyl)thio]benzyl bromide 98%
6,7-Dimethoxy-3H-quinolin-4-one