HME7细胞：人黑色素瘤复苏快细胞系

HME7细胞：人黑色素瘤复苏快细胞系 货号：GD-C4719629
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Chloro-4,6-diMethoxypyriMidine
2-Chloro-5-fluorobenzylaMine
(Methyl)triphenylphosphoniuM Iodide-d3
2,4-DiMethylthiazole
2-Fluoro-3-(trifluoroMethyl)phenylacetic acid
9-Hydroxyxanthene
4-Amino-2-fluorophenol 98%
Sodium Dodecyl Sulfate [for Electrophoresis]
Fullerene - C84, min. 99%
1-(2-methyl-1H-indol-5-yl)methanamine(SALTDATA: FREE)
2,3-Dihydropyrido[2,3-d][1,3]oxazol-2-one
(R)-2-Hydroxy-4-phenylbutyric acid
2,5-BIS(TRIFLUOROMETHYL)PHENYLACETONITRILE
1-BROMO-4-FLUORO-2-NITROBENZENE
Folic Acid Hydrate
2-Chloro-1,3-thiazole
Ethyl 2-Ethoxybenzoate
3-Methyl-5-(trifluoroMethyl)benzaldehyde
3-FluorobenzaMide, 99%
BROMOCHLOROACETIC ACID
PotassiuM SulfaMate
4,5-dicyanoimidazole
phosphoric acid, magnesium salt, hydrate (1:1:3)
Hexaphenyldisiloxane
5-Nitroisoquinoline
2,2,3,3-TETRAFLUOROPROPIONYL CHLORIDE
4,4-Difluorocyclohexanecarboxylic acid
D-^b-Prolinol, 95%
N(^e)-Benzyloxycarbonyl-L-lysine Methyl ester hydrochloride, 95%
Methyl 4-broMo-2,6-difluorobenzoate, 96%
ThiocyclaM-hydrogen oxalate
(1S,2S)-1,2-Bis(2,4,6-triMethylphenyl)ethylenediaMine dihydrochloride
4-Butylbenzyl alcohol
N,N-DiMethylphosphoraMidodichloridate, 97%
1,2,4-Triazole
2-CYANOETHYLHYDRAZINE
4-Bromo-3,5-bis(trifluoromethyl)aniline
Chloromethyldimethylphenylsilane
5-Methyl-2-heptene (cis- and trans- mixture)
4-Butoxyphenyl 4-Pentylbenzoate
Bis[4-(2-phenyl-2-propyl)phenyl]amine
Isopentyl isobutyrate
Carzenide
PalladiuM(II) acetylacetonate;Bis(acetylacetonato)palladiuM(II);Bis(2,4-pentanedionato)palladiuM(II);
2-AMino-4-hydroxy-6-MethylpyriMidine
Bifonazole
5-Hydroxy-2-Methoxyphenylboronic acid
DL-2-AMino-3-Methyl-1-butanol, 95%
4'-Fluorochalcone, 98%
Titanyl Phthalocyanine (purified by subliMation)
3'-Fluoroacetophenone
6-methylpyridine-2-carbonitrile
9-([1,1'-Biphenyl]-3-yl)-9H-carbazole
2-aminopyrimidin-5-ol
7-chloro-1H-pyrazolo[4,3-b]pyridine
Isocyanatocyclohexane
(+)-CITRONELLAL
(S)-(+)-Ketoprofen
6-DiMethylaMinopurine
Ethyl thiophene-3-carboxylate ,98%
Ethyl trans-4-Hydroxycyclohexanecarboxylate
3-Buten-1-yl Isothiocyanate
Dimethyl bromoterephthalate
1,4-Diamino-2,3-dihydroanthraquinone
1-Benzyl-2-methyl-1H-imidazole
4-(N-NONYLOXY)BENZOIC ACID
Sodium sulfosalicylate
4,5-Dichloro-3-pyridazinol
2,2′-Biquinoline-4,4′-dicarboxylic acid disodiuM salt
2-Methylundecane
1-(4-Chlorobenzyl)-1,4-diazepane
4-Phenylbutyl isothiocyanate, 95%
Oxybuprocaine hydrochloride
2-(DiMethylaMino)ethyl Methacrylate (stabilized with MEHQ)
titanium trichloride
2-Buten-1-ol, 4-chloro-3-methyl-, acetate, (Z)-
didecyldimethylammonium chloride
7-methoxyindole
FXII ELISA Kit
C4BPα ELISA Kit
Human GLP1 ELISA Kit
Human electron-transfer-flavoprotein beta polypeptide,ETFB ELISA Kit
ATF4 ELISA Kit
Rat Interleukin 4,IL-4 ELISA Kit
IL-4/CXCL4 ELISA Kit
HDV IgD ELISA Kit
Human NOS ELISA Kit
8-OHdG ELISA Kit
C.L.E.D Medium
Blood Agar Base with Potassium Tellurite
Ethyl 4-Chlorobutyrate
trans-4-(TrifluoroMethyl)cinnaMic acid, 98%
4-AcetaMidobenzyl alcohol, 97%
2-AMinobiphenyl, 97%
4,5-dicyanoimidazole
3,5-Dinitro-4-Methyl Benzoic Acid
Kalopanaxsaponin B
4-Nitrobenzonitrile

Methyl phenyl sulfone
6-HYDROXYDOPAMINE HYDROCHLORIDE
(S)-3,3-Diphenyl-1-Methyltetrahydro-1H,3H-Pyrrolo[1,2-c][1,3,2]Oxazaborole
Octanenitrile, 97%
Ethyl 4-hydroxy-3-MethoxycinnaMate, 99%
1-Pentanol
3-Quinolinecarboxaldehyde
5-METHYL-3-HEXEN-2-ONE
PotassiuM iodide
2,4-DiaMino-6-phenyl-1,3,5-triazine
N,N-DIMETHYL-4,4'-AZODIANILINE
Ethyl 3-(4-fluorophenyl)-1H-pyrazole-5-carboxylate
3-Bromo-4-fluorobenzaldehyde
4-(1-Naphthyl)phenylboronic Acid (contains varying amounts of Anhydride)
N-Carbobenzoxy-DL-serine