HME6细胞：人黑色素瘤复苏快细胞系

HME6细胞：人黑色素瘤复苏快细胞系 货号：GD-C4719628
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Boc-β-Ala-OH
(±)-3-(Boc-aMinoMethyl)piperidine
4-Hexyloxybenzoic acid
2,4-DIMETHYL-1-HEXENE
IMidazo[1,2-b]pyridazine
2-ETHYLPHENYL ISOCYANATE
2,4,6-Trimethoxybenzaldehyde
2-Nitronicotinic acid ,97%
2-[3-(Chloromethyl)phenoxy]-6-methylpyrazine, 97%
1-[5-(trifluoromethyl)-1,3,4-thiadiazol-2-yl](SALTDATA: FREE)
2,4-Diaminophenol sulfate
(3R,4S)-1-Benzoyl-3-(1-methoxy-1-methylethoxy)-4-phenyl-2-azetidinone
Cholesteryl sodium sulfate
p-Tolyl phenylacetate
Ethylidene diacetate
4-Benzoylpyridine
2-Ethyl-2-Methyl-1,3-propanediol
2-Methylthiazole-5-carbaldehyde
2,6-Difluorobenzoyl isocyanate, 80%, tech.
BROMOACETIC ACID
trans,cis-2,6-Nonadienal
2,3-dichloromaleic anhydride
Iodic acid
4-Bromobutoxytrimethylsilane
4-Methoxyquinoline
2-BUTYNAL DIETHYL ACETAL
3-Bromobenzyl alcohol
N-Boc-D-Valino
Carbonylhydridotris(triphenylphosphine)rhodiuM(I), Rh 10.0% Min
Benzyl n-butyl ether, 97%
Geraniol
2-Chlorostyrene
LEAD(II) SULFATE
N,N-DiMethylpropionaMide , 98%
2,4,7,9-TetraMethyl-5-decyne-4,7-diol
N,N-DiMethylforMaMide dipropyl acetal
(R)-(-)-2-Amino-1-butanol
Ethyl 6-amino-5-bromonicotinate 98%
1-Boc-3-Cbz-aminopyrrolidine
3-Cyanopropylphenyldichlorosilane
(S)-4-Fluorophenylglycine
4'-Methylacetanilide
2-METHOXY-5-(TRIFLUOROMETHOXY)BENZALDEHYDE
HexadecyltriMethylaMMoniuM broMide;CetriMoniuM broMide;CetriMide;Cetavlon;CTAB
DL-Methionine
1,3-Indandione
N-PhenylsucciniMide
(S)-(+)-2-Methylbutyric acid, 98%
4-Methylpyridine-3-carboxylic acid, 97%
CEPHALEXIN MONOHYDRATE
1-Benzyl L-GlutaMate
6-bromoquinoxaline
Polymethylhydrosiloxane
1H-pyarzole-3-methanol
S-phenyl chlorothioformate
2-Methylcinnamic acid
2,3,4-TRIFLUOROBENZOYL CHLORIDE
(4-Chlorophenylsulfonyl)acetaMidoxiMe
(R)-(-)-3-FLUOROPYRROLIDINE HYDROCHLORIDE
Ethyl 2-chloro-3-cyano-6-(trifluoromethyl)pyridine-5-carboxylate 97%
2-Cyano-N-methylacetamide
Thieno[2,3-b]thiophene-2-carboxylic acid, 90%
(2-fluorobenzyl)propylamine(SALTDATA: HCl)
DL-Mandelic acid
Dihydroxydimethyldiphenylmethanedisulphonic acid polymer
VANADIUM OXYTRIFLUORIDE
HEPES,1M SOLUTION
4-Fluoronitrobenzene
2,2′-Azobis(2-Methylbutyronitrile)
1,1,2,2,3,3-Hexafluoropropane-1,3-disulfoniMide
Boc-Orn(Boc)-OH
3-(p-Tolyl)propionic acid, 97%
5-Benzyloxyindole
DiMethyl D-(+)-Malate
Z-dl-leucine
(S)-(+)-5-Hydroxymethyl-2-pyrrolidinone
tetrabutyl orthosilicate
3-Chlorocinnamic acid
FXIII ELISA Kit
Ca-ATPase ELISA Kit
GLP1 ELISA Kit
Human electron-transfer-flavoprotein beta polypeptide,ETFB ELISA Kit
ATF5 ELISA Kit
Rat Interleukin 4,IL-4 ELISA Kit
IL4 ELISA Kit
HDV IgA ELISA Kit
NOS ELISA Kit
Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit
Endophytic Diazotrophs Medium
Tinsdale Agar Base
Ethyl 4-broMo-5-(trifluoroMethyl)-1H-pyrazole-3-carboxylate, 97%
trans-4-tert-Pentylcyclohexanol, 97%
4-AcetaMidopiperidine, 97%
2-AMinothiophenol, 98%
2,3-dichloromaleic anhydride
2-CARBOXY-3-ETHYL NITROBENZOATE
Sweroside
p-Hydrazinobenzoic acid

ERBIUM CARBONATE HYDRATE
N-BOC-piperidine-4-carboxylic acid

[(S,S)-MsDPEN-Ru(Mesitylene)Cl], Ru 18.5%
6-Chloro-2,3-difluorobenzaldehyde, 97%

(R)-(-)-2,2-DiMethyl-1,3-dioxolane-4-Methanol
NAPHTHOL AS-BI BETA-D-GLUCURONIDE
trans-Cyclobutane-1,2-dicarboxylic acid
TETRA-N-BUTOXYGERMANE
FMoc-L-phenylglycine
(4-Hydroxymethyl-benzyl)-carbamic acid tert-butyl ester , 97%
Pentafluorobenzenesulfonyl chloride
Bis(2-hydroxyethyl)dithiocarbamic Acid Zinc(II) Salt
4-aminotetrahydro-2H-pyran-4-carboxylic acid(SALTDATA: FREE)