HEEpiC细胞：人食管上皮复苏快细胞系

HEEpiC细胞：人食管上皮复苏快细胞系 货号：GD-C4719612
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
PotassiuM biborate
Fluchloralin
1,1,1-TRIFLUORO-2-BUTANONE
5,7-DIMETHYLISATIN
4-PHENYLSEMICARBAZIDE HYDROCHLORIDE
2,6-DIBROMOINDOSALICYLIC ACID DISODIUM SALT
2-Bromo-4,6-difluoroaniline, 99+%
Diphenyl cyanocarbonimidate ,97%
3-Fluoro-2-methylphenol 98%
1,2-Dihydroxy-4-fluorobenzene 97%
N,N-DIMETHYL-1,4-PHENYLENEDIAMINE OXALATE
HYPOTAURINE
COPPER(II) SULFIDE
BETA-PHENYLPROPIOPHENONE
Diethyl glutarate
Benzyl 4-[(MethylaMino)Methyl]piperidine-1-carboxylate
1-Ethyl-4-(Methoxycarbonyl)pyridiniuM Iodide
Methyl 3-forMylindole-4-carboxylate
(4-Benzyl-1,4-oxazinan-2-yl)Methanol, 95%
BENZO(E)PYRENE
Apigenin
3-Pyridinol, 2-methyl-
Zinc Phosphate
Isopropoxytrimethylsilane
2,6-Dinitrochlorobenzene
1-Benzyl-3-hydroxy-1H-indazole
TRANS-BETA-NITROSTYRENE
4-Chloro-Quinazoline
TetraaMMineplatinuM(II) hydrogen phosphate solution, Pt 0.5% w/w (cont. Pt)
1,4-DiforMyl, 98+%
1-Chloro-6-Phenylhexane
2-Chloro-1,4-diMethylbenzene
BIS(2-ETHOXYETHYL)AMINE
N-[BIS(METHYLTHIO)METHYLENE]-P-TOLUENESULFONAMIDE
N-(2,6-DIETHYLPHENYLCARBAMOYLMETHYL)IMINODIACETIC ANHYDRIDE
RosMarinic acid
4-Fluoro-3-(trifluoromethyl)phenacyl bromide
DEA NONOate, 98%
2-Hydrazino-nicotinic acid
tert-Butyl 4-bromo-3-methoxybenzoate 98%
2,6-DICHLORO-3-NITROPYRIDINE
3-Chlorophenylboronic acid
Procaine hydrochloride
Pigment Yellow 14
Ethyl 4-Cyclohexanonecarboxylate
Ethyl picolinoylacetate
9-Ethylcarbazole-3-carboxaldehyde N-Ethyl-N-(o-tolyl)hydrazone
6-Chloro-3-hydroxypyridazine-4-carboxylic acid
Ethyl 4,4,4-trifluoroacetoacetate, 97%
DINOCAP
2,6-Dichlorobenzaldehyde
2-phenylcyclohexanol
glucose oxidase from aspergillus niger
Dichloroglyoxime
Bis(p-hydroxyphenyl)methane
1-(2-BROMOETHOXY)-2,4-DICHLOROBENZENE
Dodecyl triMethyl aMMoniuM chloride
TRIS(3,5-DIMETHYLPHENYL)PHOSPHINE
1-Methyl-2-pyrrolidinone-d9, 97%(Isotopic)
5-Cyanothiophene-2-boronic acid
4-(4-Chlorobenzenesulfonyl)benzaldehyde
2-Methoxy-5-(trifluoromethyl)pyridine
Tridecanol (mixture of isomers)
4-(Ethylsulphonyl)benzeneboronic acid
7-FLUORO-2-METHYLQUINOLINE
Ferric chloride hexahydrate
METHYL CHLORIDE
2,4-Thiazolidinedione
2,2,2-Trifluoro-1-phenyl-ethylaMine hydrochloride
4-(4-DiethylaMinophenylazo)pyridine
2-BroMo-5h-pyrrolo[2,3-b]pyrazine
Sulfuryl chloride, 98.5%
Methyl Hexanoate
PhenylMagnesiuM BroMide (16% in Tetrahydrofuran, ca. 1Mol/L)
2-Bromo-2-methylpropane
5-Methylcyclohexane-1,3-dione
pentadecyl acetate
2-Ethylhexyl salicylate
Porcine Cortisol ELISA Kit
Mouse Cluster of differentiation 19,CD19 ELISA Kit
REG1A ELISA Kit
Human β amyloid precursor protein，βAPP ELISA Kit
TP ELISA Kit
IL-32 ELISA Kit
Equine amyloid beta peptide 1-40,Aβ1-40 ELISA Kit
Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) ELISA Kit
Human FA ELISA Kit
6-K-PG ELISA Kit
Buffered Peptone Water
BCT Enrichment Broth
Diethyl EthylMalonate
trans-Crotonic acid, 98%
Methyl 4-Acetylbenzoate
Methyl 2-aMinothiophene-3-carboxylate
3-Pyridinol, 2-methyl-
6-FLUOROQUINAZOLIN-4-ONE
1-BENZOYL
3-Nitrobenzonitrile

3-Bromophenylhydrazine hydrochloride
6-BroMopyridine-2-Carboxylic Acid Methyl Ester
2-Methyl-7-nitro-2H-1,4-benzothiazin-3(4H)-one, 97%
2-[4-(1,3,4-Oxadiazol-2-yl)phenoxy]acetaMidoxiMe, 97%
2-Cyanobenzaldehyde, 98%
4-Chloro-2-fluorophenylacetonitrile
2-Isobutoxypyridine-3-boronic acid
5-BroMo-2-cyclopropylaMino-3-nitropyridine
2-Fluoro-5-nitrobenzaldehyde
3-Cyanobenzyl Alcohol , 98%
3-Benzoylthiazolidine-2-thione , 98%
Diphenylsilanediol
Trifluoroacetic acid potassium salt
DL-Methionine Sulfone
1-(Pyridin-2-yl)piperidine-4-carboxylic acid 97%