HCECs细胞：人角膜上皮复苏快细胞系 货号：GD-C4719453
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-Di-tert-butylbenzoic acid
HexabroMobenzene
2-AMINOPERIMIDINE HYDROBROMIDE
1,3-DibroMo-5-fluoro-2-Methoxybenzene
Penicillin G PotassiuM Salt
2-Chlorobutyryl chloride, tech. 85%
4-Amino-2-methylthio-quinazoline
N-Decanoyl-D-erythro-sphingosine, synthetical, 99+%
1-(4-Nitrophenyl)pyrrolidine
Isobutyl Nitrite
3-Methyl-5-nitro-1H-indazole
2-HYDROXY-6-(TRIFLUOROMETHYL)PYRIDINE
Tecloftalam
Dimethyl sulfate
1,1-Cyclohexanediacetic acid
2-(EthylaMino)ethanol
4,4-Difluorocyclohexanecarboxylic Acid
2'-AMinobiphenyl-3-carboxylic acid
DiMethyl nitroterephthalate, 99%
1,2,4,5-TETRACHLOROBENZENE
5-Nitroguaiacol
7,7-dichlorobicyclo[4.1.0]heptane
4,4'-sulfonylbis(2-nitrophenol)
cholesteryl hemisuccinate free acid
Benzophenone oxime
1-Hexyl-3-methylimidazolium tetrafluoroborate
10-CHLORO-9-ANTHRALDEHYDE
2,3-Dihydro-1H-Pyrrolo[2,3-B]Pyridine
Gold(III) hydroxide, Au 79% Min
3-Chloro-4-Methylbenzenesulfonyl chloride, 99%
2-AMinobenzeneboronic acid pinacol ester
2-Hydroxy-4-Methyl-5-nitropyridine
TETRANITROPHENOLSULFONPHTHALEIN
N-EthylMorpholine
2-Ethyl-4-Methyl thiazole
1-AMinonaphthalene
1-(4-Bromo-2-fluorobenzyl)
Silver powder (99.9%)
2-Chloro-6-methoxyisonicotinamide , 95%
Methyl 3-bromo-5-tert-butylbenzoate
Prasugrel
2,3,6-TRICHLOROPHENOL
8-Aminoquinoline
Carbon tetrachloride;PerchloroMethane;BenzinoforM;Necatorina
1-Benzyl-2-(chloroMethyl)-1H-iMidazole hydrochloride
LitMus
1-Ethynyl-4-(phenylethynyl)benzene
2-[2-(4-Chlorophenyl)-1,3-thiazol-4-yl]acetic acid
S-Methyl Methanethiolsulfonate, 97%
P,P-DDT
2-(PhenylsulfonylMethyl)benzonitrile
1-Methylisoquinoline
2-Amino-1-(3,4-difluorophenyl)ethanol
2-(2'-Hydroxy-5'-methyl-phenyl)benzotriazole
2-Bromochlorobenzene
(R)-(-)-N-METHYL-1-PHENYL-2-(1-PYRROLIDINO)ETHYLAMINE
5,10,15,20-TETRAPHENYL-21H,23H-PORPHINE IRON(III) CHLORIDE
ALPHA-PHENYLCYCLOPENTANEACETIC ACID
SCANDIUM SULFATE
5-Bromo-2-methylaniline
2-Fluorophenyl methyl carbinol
4-Azidophenacyl bromide
5-(Acetylamino)-2-nitrobenzoic acid , 97%
4-(Trifluoromethyl)benzylamine
2-Fluoro-3-methylaniline
Diphenyl N-cyanocarbonimidate
Zinc fluoride
D-Sorbitol
1-Nitrosopyrrolidine
o-Toluidine Hydrochloride
1-Methyl, 99%
N'-Hydroxy-4-(trifluoroMethyl)pyridine-3-carboxiMidaMide, 95%
Diloxanide furoate
3,5-DiMethoxybenzyl Alcohol
Amines, C16-18-alkyldimethyl
2,2-Dimethyl Cyclopropyl Carboxylic Acid
2-Nitropyridine-4-carboxylic acid
3-(Bromoacetyl)pyridine hydrobromide
HSF1 ELISA Kit
Mouse FBXO32 (F-Box Protein 32) ELISA Kit
Human hnRNP R ELISA Kit
Human D5R/DRD5 (Dopamine Receptor D5) ELISA Kit
T4 ELISA Kit
IL-12/P70 ELISA Kit
Chicken Tumor necrosis factor α,TNF-α ELISA Kit
Human protein phosphatase 1 regulatory subunit A,PPP1R1A ELISA Kit
PDGFB ELISA Kit
TMED1 ELISA Kit
Autoinduction Luria-Bertani Broth Medium
Autoinduction Luria-Bertani Broth Medium
Nickel(II) Acetate Tetrahydrate
trans-DibroMobis(triphenylphosphine)palladiuM(II), Pd 13.4%
4-n-Butylbenzeneboronic acid, 98%
2-PhenylethylaMine Hydrochloride
7,7-dichlorobicyclo[4.1.0]heptane
Ammonium magnesium sulfate
cyanidin
2-Aminoquinoline
N-Acetylneuraminic acid
TERT-BUTYL 5-BROMOINDOLE-1-CARBOXYLATE
L-(+)-GlutaMic Acid Hydrochloride
4-n-Butoxyphenol, 98%
3-Fluorobenzotrifluoride, 99%
3-Methoxy-4-(trifluoroMethyl)benzoic acid, 97%
2-Cyanobenzaldehyde
n-Octacosane
2-AMinooxazole-4-carboxylic acid
3-Chlorocarbonylphenylboronic anhydride
4-Cbz-AMino-3-chlorophenylboronic acid, pinacol ester
2,1,3-Benzothiadiazole-4-carboxylic acid ,97%
Chelidonic acid monohydrate, 96%
D-Idose ,99%
1,1,1,3,3,5,5-Heptamethyltrisiloxane
