GT1-1细胞：小鼠垂体瘤传代细胞系

GT1-1细胞：小鼠垂体瘤传代细胞系 货号：GD-C4619076

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
O(1),O(3)-Bis(ethoxycarbonylMethyl)-p-tert-butylcalix[4]arene
5-Fluoro-2-iodoaniline
1-PHENYL HYDROCHLORIDE
4-CHLOROBENZYL ISOTHIOCYANATE
8-FLUORO-4-HYDROXY-2-(TRIFLUOROMETHYL)QUINOLINE
2,5-DIBROMO-3,6-DIFLUOROBENZENESULFONYL CHLORIDE
N-(4-Chlorobenzoyloxy)-4-nitrobenziMidaMide
4-(chloromethyl)-2-methyl-1,3-oxazole(SALTDATA: FREE)
1-[3-(1H-tetrazol-5-yl)phenyl]methanamine(SALTDATA: HCl)
N-tert-Butyl-N-ethylnitrosamine
3-Aminopropyltris(trimethylsiloxy)silane
2'-CHLORO-6'-FLUORO-3'-METHYLACETOPHENONE
BENZYL GLYCIDYL ETHER
1-Boc-3-piperidinecarboxylic acid
1-Phenyl-1-cyclopropanecarbonitrile
5,6-Dichloronicotinic acid
5,6-Dichloro-2-MethylbenziMidazole
N-(2-Hydroxyethyl) 4-broMobenzenesulfonaMide
TriMethylaMine, 1M solution in THF, AcroSeal
PROPYLENE GLYCOL METHYL ETHER
9-Mesityl-2,7,10-triMethylacridiniuM Perchlorate
Methyl 2-chlorobenzoate
Benzylidencyclohexanone; 98%
Phosphonitrilic chloride trimer
4-fluoro-1H-indazole
2,3-Dihydroxybenzaldehyde
6-CHLORO-2,4-DIMETHOXYPYRIMIDINE
VinyltriMethylsilane
SodiuM tetraborate, anhydrous, 99.95% (Metals basis)
2'-Fluoro-5'-Methylacetophenone, 97%
1,3-DiaMino-4-(5-broMo-2-pyridylazo)benzene
Methyl palMitelaidate
(1S)-(+)-DiMenthyl succinate
Poly(styrene-divinylbenzene), aMinoMethylated, 1% cross-linked, 200-400 Mesh
2-AMino-6-Methyl-4-pyriMidinol
SodiuM tetraborate decahydrate
(+)-2-Amino-2-deoxy-D-glucose hydrochloride 98+%
α-1,6-D-Mannobiose ,95%
Diethyldiselenide
2-Chloro-3-amino-4-(trifluoromethyl)pyridine
2-Amino-4-phenylbutane
2,4-Dimethoxyaniline
(2S,3S)-(-)-BIS(DIPHENYLPHOSPHINO)BUTANE
2,7-Dibromo-9H-fluoren-9-one
4-(TrifluoroMethyl)phenyl isothiocyanate
1-(3-DiMethylaMinopropyl)
Glycylglycine Hydrochloride Monohydrate
1-(3-AMinophenyl)pyrrolidin-2-one
(?)-alpha-Methoxy-alpha-trifluoroMethylphenylacetyl chloride, 97%
ROSOLIC ACID
4-Ethylheptane
2-Methyl-2-thiazoline
ethyl 1H-tetrazole-5-acetate
5-Acetylvaleric acid
Tri-n-butylchlorosilane
AMMONIUM
1,3,5-TRIBENZOYLBENZENE
6-Chloroacetyl-2H-1,4-benzothiazin-3(4H)-one
Methyl 6-forMylpicolinate
3,4-Dimethoxybenzeneboronic acid
Methyllithium, 1.6 M sol. in diethyl ether (± 5% w/v), AcroSeal
Hexaamminenickel(II) chloride, 98%
(R)-?-(p-Trifluoromethylphenyl)alanine
2-BROMOETHYL ISOTHIOCYANATE
4-Pyrimidinol
4-Amino-2-picoline
2,5-Dichlorobenzo-1,4-quinone
3-BroMo-4-Methylaniline
10-Butyl-2-chloro-9(10H)-acridone
3-Butoxy-5-(trifluoroMethoxy)phenylboronic acid
alpha-BroMostyrene, 95%, stabilized
2-Ketoglutaric acid, 98%
Chirabite-AR
Ethyl 5-ChloroMethyl-2-furancarboxylate
methyl (2E)-3-(2-nitrophenyl)prop-2-enoate
(1R,3S)-N-BOC-1-Aminocyclopentane-3-carboxylic acid
5-Bromo-3-cyanoindole
Mordant Red 15
PE ELISA Kit
Mouse FUCA (Alpha-L-Fucosidase, Tissue) ELISA Kit
EGR4 ELISA Kit
Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
Hen egg phosvitin phosphopeptides,PPP ELISA Kit
Smad7 ELISA Kit
Chicken CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit
Human secretin receptor,SR ELISA Kit
Human VEGF-B ELISA Kit
AT1R-AA ELISA Kit
Iso-Sensitest Agar
Tyrobutyricun Broth Base
Isobutyraldehyde
GlycinaMide Hydrochloride
5-Chloro-2-pentanone
2-Fluoro-N,N-diisopropylbenzaMide, 97%
Methyl 2-chlorobenzoate
Solvent Yellow 114
Fatty alcohol polyoxyethylene ether, sodium sulfate
5-Bromo-2-fluorobenzotrifluoride

2-Aminobutanol
4-Amino-N-methylbenzenemethanesulfonamide
2-Chloro-4-iodoaniline
PalladiuM(II) sulfide, 99% (Metals basis), Pd 72% Min
4-BroMophenoxyacetonitrile, 97%
N,N-DiMethylcyclohexylaMine, 98+%
Alizarin
1,3-Di-p-tolylcarbodiiMide
Phosphorous acid
rac NorMetanephrine Hydrochloride
IMinodiacetonitrile
1,3-PROPANEDIOL DI-P-TOLUENESULFONATE
1-(2-Chloroethyl)piperidine Hydrochloride
Ethyl trifluoropyruvate 98%
(R)-?-(4-Fluorophenyl)alaninol