KALS-1细胞:人神经胶质瘤传代细胞系 货号:GD-C4620165
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1,2,3-TribroMopropane, 98%
DL-N-AcetylhoMocysteine thiolactone
PHENYLSELENOCYANATE
LithiuM nitrate
5-Fluorouridine
TriethylenediaMine
4-Methyl-3-nitroanisole
(5-Methyl-3-trifluoromethylpyrazol-1-yl)acetic acid
3,4,5-Trimethoxyphenylacetonitrile, 97%
3-Cyclohexyl-1-propanol
4-sec-Butoxy-2-butanone
1,2-Diethoxybenzene
Tin(II) fluoride
Thiamine disulfide
TetraethylaMMoniuM chloride hydrate
Methyl isobutyrate
DiMesitylaMMoniuM Pentafluorobenzenesulfonate
N,N-DiMethyl 3-broMobenzenesulfonaMide
TribenzylaMine, 99+%
Tween(R) 20 (Polysorbate), NF, Ph Eur
N-CyclohexylethanolaMine
2-Methyl-5-quinolinol
1,2-Dibromocyclohexane
Hexyldichlorosilane
2-Ketoglutaric acid
2,3-DIAMINOTOLUENE
6,7-Dihydro-5-Methyl-5(H)-cyclopentapyrazine
Butyric acid, 99+%
CinnaMyl chloride, 95%, stab. with sodiuM carbonate
1-Dodecyne, 97%
ThioMorpholine
Diethyl DL-Malate , 98%
2-(4-Morpholinyl)-5-(trifluoroMethyl)aniline, 95%
Propyl acetate
5-AMino-2-Methylphenylboronic acid, pinacol ester
4-(2-(DiMethylaMino)ethylcarbaMoyl)phenylboronic acid, pinacol ester
Bromonitromethane, tech., 90%
(3-Thien-2-ylphenyl)methanol, 97%
4-Chloro-2-methylbenzaldehyde
2-Propylfuran
4-Methyl-3-nitroaniline
4-(4-FORMYL-3-METHOXY-PHENOXY)-BUTYRIC ACID
4-BENZYLOXY-3-INDOLEACETONITRILE
(R)-(-)-2-AMINO-3-METHYLBUTANE
1-Methylnaphthalene
1,4-Bis(butyryloxy)-2-butene
3-Ethoxy-4-Methoxyphenylboronic acid
Dibutyl Maleate, 97%
1,2,3,4-Tetrahydro-6-quinolinecarboxylic acid, 97%
Dansyl-L-valine
(S)-(-)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl
naphthalene-2,6-dicarboxylic acid
7-fluoro-1H-indazole-3-carboxylic acid
N(alpha)-(2,4-dinitro-5-fluorophenyl)-L-valinamide
[3,5-bis(trifluoromethyl)phenyl]acetonitrile
N-Benzylacrylamide
PHENYLSULFINYLACETIC ACID ETHYL ESTER
N-TETRACONTANE
1,2,4,5-TETRAKIS(TERT-BUTYLTHIO)BENZENE
1-Isocyanato-2,4-dimethylbenzene
4-Benzyloxy-3-nitroacetophenone ,98%
2,3- Dihydro-4-(trifluoroacetyl)furan
2,4,6-Tri-tert-butylnitrobenzene
1-Cbz-4-hydroxymethylpiperidine
Methyl 2-iodobenzoate
2-Fluoro-4-methoxyacetophenone
Stevioside
3-AMino-2-Methylbenzoic acid
1,4-Butanedithiol
5-(3-Methoxyphenyl)nicotinic acid
6-AMino-2-naphthoic acid, 90%
5-(TrifluoroMethyl)-1H-indazole, 97%
5-Methyl-2-hexanone
D-(+)-Maltose Monohydrate
1,2,3,4-Tetrahydro-1-naphthol
Methyl 3-oxo-3-(3-furanyl)propanoate
ethyl 4-[[1-[(4-fluorophenyl)methyl]-1H-benzimidazol-2-yl]amino]piperidine-1-carboxylate
S(-)-glycidol
Porcine plasminogen activator inhibitor,PAI ELISA Kit
βGAL ELISA Kit
CENPB ELISA Kit
Human SNX6 ELISA Kit
Chicken very low density lipoprotein,VLDL ELISA Kit
Rat Substance P,SP ELISA Kit
Chicken NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
TSH ELISA Kit
Human ANG II R1-Ab (Angiotensin II Receptor 1 Antibody) ELISA Kit
CE ELISA Kit
Poly-Peptone Extract Medium
Bluestone Medium
Isoquinoline-4-carboxaldehyde, 97%
LanthanuM(III) perchlorate hexahydrate
5-Chlorothiophene-2-carboxylic acid, 98%
1-Fluoro-2-Iodobenzene
2-Methyl-5-quinolinol
Ammonium titanyl oxalate monohydrate
1-Chloroethyltrimethylsilane
Perfluoroheptane
1-Benzyl-3-pyrrolidinone
4-METHYL-1,3-DIOXANE
2-(ChloroMethyl)-2-Methyloxirane
N^d-Boc-N^a-FMoc-D-ornithine, 95%
Fluorocyclohexane, 97%
4-Methyl-3-nitrobenzonitrile, 98%
Tris(pentafluorophenyl)borane
1,4-DIBROMO-2,3-BUTANEDIONE
PLUMBAGIN
4-(5-BroMopyridin-3-ylsulfonyl)Morpholine
OxaMic hydrazide
ZINC TUNGSTATE
2-Bromo-4-fluorobenzoic Acid
2-(Diethylamino)-N-(2,6-dimethylphenyl)-acetamide, 97.5%
N-Sulfinyl-p-toluenesulfonamide