HMC细胞：人肾小球系膜传代细胞系

HMC细胞：人肾小球系膜传代细胞系 货号：GD-C4619624
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
TAPS
Clofentezine
2,2,5,5-TETRAMETHYL-3-PYRROLIN-1-OXYL-3-CARBOXYLIC ACID N-HYDROXYSUCCINIMIDE ESTER
2-(PHENYLSULFONYL)ACETOPHENONE
Bis(tetrabutylaMMoniuM) dichroMate
9-Fluorenone
Ethyl Propionate
Cyclooctanecarbaldehyde
4-Ethylbiphenyl-4'-carboxylic acid, 98%
2-(Methylsulfonyl)aniline hydrochloride
2-(7-Aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate
Tween 20
trans-5-Amino-6-hydroxy-2,2-dimethyl-1,3-dioxacyloheptane
2,2,2-TRIFLUOROETHYL METHYL ETHER
2-BroMopropionyl chloride
(1-Methyl-1H-pyrazol-3-yl)Methanol
4,6-O-Ethylidene-alpha-D-glucopyranose
4-Methylphenylglyoxal hydrate
TriMethylstannyldiMethylphenylsilan, 96%
BisMuth AtoMic Absorption Standard
Bis(triMethylsilyl) Sulfide
Glutarimide
Chlorine
Thiazol Yellow G
N,N-diphenylformamide
2-Aminobenzophenone
6-BROMO-2,2-DIMETHYLHEXANENITRILE
Tetra-n-Propoxysilane
Bis(dicyclohexylphosphino)Methane, 97+%
2,3-Dichloropyrazine, 98%
Gastrodin
3-MethoxydiphenylaMine
1-Benzyl D-GlutaMate , 98%
N,N-DI-N-PROPYLANILINE
4-(2-Piperazinyl)phenol, 95%
5-TERT-BUTYL-2-METHYLTHIOPHENOL
Tetrahydro-2H-pyran-4-ylmethanol, 97%
4-(Difluoromethoxy)-3-hydroxybenzaldehyde 97%
Formononetin, 99%
1-ethyl-1H-pyrazol-3-amine(SALTDATA: FREE)
METHYL PHENYLPROPIOLATE
2,5-BIS(TRIFLUOROMETHYL)BENZENESULFONYL CHLORIDE
2-CHLORO-4-(TRIFLUOROMETHYL)PYRIMIDINE-5-CARBONYL CHLORIDE
N-Boc-4-piperidinemethanol
BroModichloroMethane
tert-AMylbenzene
(S)-4-(2-Hydroxyethyl)-2,2-diMethyl-1,3-dioxolane
3-(4-BOC-piperazino)-4-nitroanisole
7-BroMobenzo[b]thiophene, 97%
Mecobalamin
2,7-Diphenyl[1]benzothieno[3,2-b][1]benzothiophene
2-Butyrylfuran
Tris(6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-3,5-octanedionato)praseodymium
N-phthaloyl-L-phenylalanine
4-Bromophenylacetic acid
COPPER(II) SELENATE
Pyruvonitrile
N-HEPTADECANE
1-BroMo-4-chloro-3-(Morpholinocarbonyl)benzene
3-(Methylthio)-1-hexanol, 97%
2-(2-Fluorophenoxy)-3-nitropyridine , 97%
1-Bromoundecane
cis-3-Hexen-1-yl Salicylate
TETRAPHENYLLEAD
1,2-Diformyloxyethane
2-Cyclohexen-1-one
2,2'-(P-TOLYLIMINO)DIETHANOL
alpha-Terpinene
2,2',5,5'-Tetrachlorobenzidine
2,3,5,6-Tetrafluoro-4-Mercaptobenzoic Acid
2-Methoxyphenyl isocyanate, 99%
N,N,N',N'-TetraMethylbenzidine, 97.5%
Diflorasone diacetate
Allyl Glycidyl Ether
2-(2-fluorophenoxy)aniline
Famciclovir
Serine, N-[(1,1-dimethylethoxy)carbonyl]-O-(phenylmethyl)-
sec-butyl tiglate
VCL ELISA Kit
Mouse CEBPD (CCAAT/Enhancer Binding Protein Delta) ELISA Kit
Human ghcagons-like pepfide 1,GLP-1 ELISA Kit
Amylase ELISA Kit
RELA ELISA Kit
IL4 ELISA Kit
IL1β ELISA Kit
Human Azidothymidine,AZT ELISA Kit
NO ELISA Kit
HSPA5 ELISA Kit
Nodule Bacteria Medium YM
Bordet-Gengou Agar Medium Base
Ethyl 6-(trifluoroMethyl)iMidazo[1,2-a]pyridine-2-carboxylate, 95%
trans-4-Ethyl-4'-(4-n-propylcyclohexyl)biphenyl, 97%
4-Acetylbutyric acid, 97%
2-AMinoiMidazole sulfate, 98%
Glutarimide
cis,cis-1,3,5-cyclohexanetricarboxylic acid
Isopropyl 3-Aminocrotonate
4-(Dimethylamino)phenol

METHYL 3-AMINOTHIOPHENE-4-CARBOXYLATE HYDROCHLORIDE
4-Tolyl ether
Tri-P-Tolylphosphine
2-Fluoro-3-(trifluoroMethyl)benzeneboronic acid, 97%
1-(2-Methoxybenzoyl)-4-Methylpiperidine, 97%
4-Fluorosalicylaldehyde
Mercuric aMMoniuM chloride
4,4'-Diethoxybiphenyl , 99%
3-CHLORO-2,6-DIFLUOROBENZOYL CHLORIDE
1,4-THIOXANE
3-HydroxycinnaMic acid
Palladium(II) acetate/1,1'-bis(di-t-butylphosphino)ferrocene/potassium phosphate admixture [Catkit single-use vials - 2.02 wt% Pd(OAc)2]
Benzo[b]furan-3-carbonyl chloride , 97%
Methyl 2-amino-2-[4-(trifluoromethyl)phenyl]-acetate hydrochloride
(4-amino-1-naphthyl)amine(SALTDATA: 2HCl)