HUC细胞：人尿道上皮传代细胞系

HUC细胞：人尿道上皮传代细胞系 货号：GD-C4618272

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1-(4-BroMobenzyl), 97%
AcetoguanaMine
2,4-DIMETHYL-4-PENTEN-2-OL
2-CHLORO-6-ETHOXYPYRIDINE
Hexyl isocyanate
Ethyl 4-diMethylaMinobenzoate
2-BroMo-4-fluorothioanisole
Cyclopropanecarboxylic acid
Phthalic Acid Bis(2-butoxyethyl) Ester
5-Bromo-2-iodobenzoic acid
1-Cyclohexyl-1-butanol
4-METHOXYPHENYLPHOSPHONIC ACID DIETHYL ESTER
Boc-O-methyl-L-tyrosine
2-METHYL-5-NITROBENZONITRILE
Ethyl pipecolinate
2,5-Dichloropyridine
3,3'-Dichloro-4,4'-diisocyanatobiphenyl
5-BroMo-6-MethoxybenzoiMidazole
p-Methylstyrene, 98%, stabilized
Tartrazine
1-AdaMantaneacetic Acid
hydroflumethiazide
Dimethylaminoethyl methacrylate
3-Thiophenecarboxaldehyde
Di-n-butyl(maleate)tin
2-Nitrobenzeneboronic acid pinacol ester
BENZENESULFINIC ACID
Lauryl chloroforMate
Dodecacarbonyltetracobalt, 98% Min
Triphenylphosphine, powder, 99%
MGK 264
3-Fluorobenzoyl Chloride
1-(Phenylsulfonyl)-1H-iMidazole
ZIRCONIUM SILICATE
ThioMorpholine-1,1-dioxide
4-AMinophthalonitrile
2-(Dimethylamino)ethanol Hydrogen L-(+)-Tartrate
Pent-2-enoic acid 97%
2-Di-t-butylphosphino-2',4',6'-tri-i-propyl-1,1'-biphenyl, min. 98% t-butylXPhos
n-Decyltrimethoxysilane
(+)-Pilocarpine hydrochloride
2-METHOXYETHYL ISOTHIOCYANATE
2,3,4,5,6-PENTAFLUOROBENZENEBORONIC ACID
SodiuM Lactate
6-Methoxyindole
4-Mercaptopyrazolo[3,4-d]pyriMidine
N-SucciniMidyl 3-MaleiMidopropionate [Cross-linking Reagent]
1-AMinopyrene, 97%
4-Chlorobenzhydrylchloride, 99+%
2-Naphthoyl chloride
4-EthoxycouMarin
(6-chloropyridin-3-yl)(morpholin-4-yl)methanone
4,4'-Bis(methoxycarbonly)-2,2'-bipyridine
Dimethyldichloropropane
2-(AdaMantan-1-yl)isoindoline-1,3-dione
TRIS(2-AMINOETHYL)AMINE
3-(N,N-DiMethylaMino)phenylboronic acid
4-Hydroxy-3,5-diMethylphenylboronic acid
SEBACIC ACID DISODIUM SALT
Rubidium nitrate (99%-Rb)
Perfluorocyclohexanecarboxylic acid
Disodium 3-Hydroxy-2,7-naphthalenedisulfonate
1-Chloro-2-methyl-2-phenylpropane, 98%
(3R)-(+)-3-(METHYLAMINO)PYRROLIDINE
Clindamycin
Zirconium sulphate
PALMITOLEIC ACID
Benzyl 4-(chlorocarbonyl)tetrahydro-1(2H)-pyridinecarboxylate
(-)-Di-p-anisoyl-L-tartaric Acid
Nonadecafluorodecanoic Acid
H-D-Lys(boc)-oh
2-(4-AMinophenyl)-6-Methyl-1,3-benzothiazole-7-sulfonic acid, 97%
(S)-(+)-ALPHA-METHOXY-ALPHA-TRIFLUOROMETHYLPHENYLACETYL CHLORIDE
Tris(2-carboxyethyl) Isocyanurate
Boc-Orn-OH
Boc-O-methyl-L-threonine
dodecylmagnesium bromide
1,4-Di-tert-butyl-2,5-dimethoxybenzene
CTSL ELISA Kit
LH ELISA Kit
Human TPA ELISA Kit
Human BMP-5 ELISA Kit
HSP90αA1 ELISA Kit
IGFBP1 ELISA Kit
CDH5 ELISA Kit
GAL2 ELISA Kit
Human cytb561 ELISA Kit
sCD21 ELISA Kit
Tryptic Soy Agar
Murashige & Skoog Medium without Agar and Sucrose
PalMitic acid hydrazide, 99%
Sulfanilamide
Ethyl 5-broMo-2-chlorobenzoate, 98+%
2-HydroxypyriMidine hydrochloride, 98+%
hydroflumethiazide
2-Fluoropropionic acid
(1S)-1-(5,8-dihydroxy-1,4-dioxo-1,4-dihydronaphthalen-2-yl)-4-methylpent-3-en-1-yl 3-methylbut-2-enoate
Propionic acid

BARIUM THIOCYANATE TRIHYDRATE
3-BROMO-4-HYDROXYBENZOIC ACID HYDRATE
10-Hydroxy-2-decenoic acid
4-(1H-Pyrazol-1-yl)benzoic acid, tech. 90%
2,4-Dioxo-1,2,3,4-tetrahydroquinazoline-6-sulfonyl chloride, 97%
PyMetrozin
MetaMitron
ETHYL 4-(TRIFLUOROMETHYL)BENZOATE
Benzophenone
N,N-Diethyl-1,1,1-triMethylsilylaMine
4-BroMo-2-Methylbenzoic acid
3-(2-Methylpyrimidin-4-yl)benzenesulphonyl chloride
Rubidium carbonate (99.8+%-Rb)
m-Xylylene Diisocyanate
6,7-Dihydro-5H-quinolin-8-one